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  • DNA sequencing and bioinformatics - BaseClear
    Biology Synthetic DNA libraries Gene synthesis Custom cloning PCR cloning Mutagenesis Lab Products Zymo Research Epigenetics DNA purification RNA purification Other products Sample kits Epigenetic services Bio Basic Gene Synthesis Protein expression and purification Peptide synthesis Custom antibody service Kisker Biotech Lab disposables Lab equipment Microparticles Industries Industrial Biotechnology Food Ingredients Pharma Health Water Environment Clinical Diagnostics laboratories Agro Sciences Academic Research institutes About BaseClear Our company Meet our team History Partners Distributors Why BaseClear Reasons to work with BaseClear Research development Quality assurance Technologies Information News Ordering Career Jobs Internships Contact Contact details Contact form Request quotation Order forms Blog Next Gen sequencing Complete solutions for sequencing projects on HiSEQ2500 MiSEQ and PacBio platforms Read more Industrial Biotechnology We support biotech companies to develop their sustainable bio based solutions Read more Food Ingredients Genomic research solutions for clients working in dairy food beverage and ingredients industries Read more Agro Sciences Research in agro sciences is increasingly driven by efficient next generation sequencing solutions Read more IF YOU NEED TO BE SURE We offer specialized DNA services and solutions for our clients in 30 countries WHY BASECLEAR Latest News BaseClear contributes to knowledge of biodiversity and water assessment More news

    Original URL path: http://www.baseclear.com/ (2016-02-12)
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  • Genomics services - BaseClear
    purification RNA purification Other products Sample kits Epigenetic services Bio Basic Gene Synthesis Protein expression and purification Peptide synthesis Custom antibody service Kisker Biotech Lab disposables Lab equipment Microparticles Industries Industrial Biotechnology Food Ingredients Pharma Health Water Environment Clinical Diagnostics laboratories Agro Sciences Academic Research institutes About BaseClear Our company Meet our team History Partners Distributors Why BaseClear Reasons to work with BaseClear Research development Quality assurance Technologies Information News Ordering Career Jobs Internships Contact Contact details Contact form Request quotation Order forms Blog Home Our services Lab Products Industries About BaseClear Blog Next Gen sequencing Bioinformatics Sanger sequencing Microbiology Synthetic Biology BASECLEAR SERVICES Since 1993 we offer a complete range of genomic metagenomic transcriptomic and bioinformatic services for life scientists working in biotechnological and bio pharmaceutical companies academic and research institutes as well as agro and food industries In addition we offer applied services in microbiology e g bacterial and fungal identifications microbial typing or microbial profiling and in synthetic biology e g making of DNA libraries gene synthesis etc Our goal is to be a true partner for our clients so we can assist them to reach their goals as quickly and economically as possible while maintaining the

    Original URL path: http://www.baseclear.com/genomics (2016-02-12)
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  • Next Generation sequencing - BaseClear
    our team History Partners Distributors Why BaseClear Reasons to work with BaseClear Research development Quality assurance Technologies Information News Ordering Career Jobs Internships Contact Contact details Contact form Request quotation Order forms Blog Home Our services Lab Products Industries About BaseClear Blog Next Gen sequencing Genome De Novo Re sequencing Transcriptome sequencing Small RNA sequencing Metagenomics Microbial profiling Bioinformatics Sanger sequencing Microbiology Synthetic Biology NEXT GEN SEQUENCING BaseClear offers complete service packages based on innovative Next Generation Sequencing technologies These technologies make it possible to sequence for instance complete genomes within weeks at a fraction of the costs of conventional sequencing technologies BaseClear offers sequencing services on the Illumina systems HiSEQ2500 and MiSEQ in combination with the PacBio RS II system With these technologies we can offer an unmatched combination of read lengths paired end size ranges number of reads number of tags and short delivery times These systems have the power and flexibility to enable a wide range of genome scale applications at the lowest cost per base ADVANTAGES OF OUR NEXT GEN SEQUENCING SERVICES Our specialists can be of excellent assistance with the experimental design of your projects Available platforms PacBio RS II Illumina HiSEQ2500 and Illumina MiSEQ

    Original URL path: http://www.baseclear.com/genomics/next-gen-sequencing (2016-02-12)
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  • Genome De Novo sequencing - BaseClear
    be sequenced for the first time we offer our De Novo Sequencing service With our many years of experience highly qualified specialists and state of the art technologies we can assist you with even the most challenging De Novo sequencing projects We can provide complete start to finish project management with customized analysis and data reporting Our highly qualified staff is always willing to discuss your project to see how we can help you reach your research goals as fast as possible HYBRID STRATEGY For most De Novo sequencing projects we recommend a hybrid strategy With the SMRT technology Single Molecule Real Time sequencing of the PacBio RS II platform we can offer long sequence reads up to 30 kbp and average of 3 4 kbp s read to create a backbone We will also sequence your genome on the Illumina HiSEQ2500 platform single or paired end reads to get sufficient coverage and thus reliable results Our in house developed SSPACE Longread plugin is capable of merging the long and short read data into one hybrid assembly Final gaps if any can be eventually closed using direct Sanger sequencing and or long range PCR based approaches EXPERTISE IN MICROBIAL GENOMES Although we have completed many projects involving human animal or plant genomes we have a strong focus on microbial genomes bacteria fungi yeast algae viruses with genome sizes ranging up to 50 Mbp We have sequenced and assembled significant numbers of bacteria including E coli Bacillus Lactobacillus Lactococcus Actinomycetes Neisseria fungi such as S cerevisiae Aspergillus Tricoderma and viruses Influenza Adenovirus etc Fill in our contact form to discuss your microbial genome sequencing project with one of our specialists WHY CHOOSE OUR DE NOVO SEQUENCING SERVICE Best sequencing strategy based on a combination of PacBio RS II and Illumina HiSEQ

    Original URL path: http://www.baseclear.com/genomics/next-gen-sequencing/de-novo (2016-02-12)
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  • Re-sequencing - BaseClear
    Jobs Internships Contact Contact details Contact form Request quotation Order forms Blog Home Our services Lab Products Industries About BaseClear Blog Next Gen sequencing Genome De Novo Re sequencing Transcriptome sequencing Small RNA sequencing Metagenomics Microbial profiling Bioinformatics Sanger sequencing Microbiology Synthetic Biology GENOME RE SEQUENCING Our re sequencing service is helpful to discover and confirm SNPs to identify chromosomal rearrangements to map break points and to detect rare variants With our many years of experience highly qualified specialists and state of the art technologies we offer re sequencing projects with fast delivery times the highest quality and at competitive prices We can provide complete start to finish project management with standard or customized analysis and data reporting Our highly qualified staff is always willing to discuss your project to see how we can help you reach your research goals as fast as possible EXPERTISE IN MICROBIAL GENOMES Although we have completed many projects involving human animal or plant genomes we have a strong focus on microbial genomes bacteria fungi yeast algae viruses with genome sizes ranging up to 50 Mbp We have sequenced and assembled significant numbers of bacteria including E coli Bacillus Lactobacillus Lactococcus actinomycetes Neisseria fungi and yeast such as S cerevisiae Aspergillus Tricoderma and viruses Influenza Adenovirus etc WHY OPT FOR OUR RE SEQUENCING SERVICE Our specialists can help you to decide which strategy should be used for best results The final sequencing strategy is determined in close collaboration between the customer and our product specialist Guaranteed fast delivery times possible with our premium RAPID run services Strong focus on microbial genomes Project size ranging from one genome to several hundreds genomes Multiplex options available to sequence multiple samples in a single lane by adding sequence indexes to a library Delivery time is dependent on when

    Original URL path: http://www.baseclear.com/genomics/next-gen-sequencing/re-sequencing (2016-02-12)
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  • Transcriptome sequencing - BaseClear
    Lab equipment Microparticles Industries Industrial Biotechnology Food Ingredients Pharma Health Water Environment Clinical Diagnostics laboratories Agro Sciences Academic Research institutes About BaseClear Our company Meet our team History Partners Distributors Why BaseClear Reasons to work with BaseClear Research development Quality assurance Technologies Information News Ordering Career Jobs Internships Contact Contact details Contact form Request quotation Order forms Blog Home Our services Lab Products Industries About BaseClear Blog Next Gen sequencing Genome De Novo Re sequencing Transcriptome sequencing Small RNA sequencing Metagenomics Microbial profiling Bioinformatics Sanger sequencing Microbiology Synthetic Biology TRANSCRIPTOME SEQUENCING RNA SEQ With our mRNA Seq service you can unlimited discover and profile the entire transcriptome With no probes or primers to design the mRNA Seq method can deliver unbiased and unparalleled information about the transcriptome BaseClear offers strand specific transcriptome analysis which is based on sequencing on the Illumina platforms TRANSCRIPTOME SEQUENCING PROJECTS We have completed many transciptomic sequencing projects involving for instance human and plant samples In addition we have a strong focus on microbial organisms bacteria fungi yeast algae viruses We have special transcriptomics protocols available for bacterial and fungal samples WHY CHOOSE BASECLEAR S TRANSCRIPTOME SEQUENCING SERVICE Our specialists can be of assistance with the experimental design of your projects The sequencing strategy is determined in close collaboration between the customer and our product specialist Guaranteed fast delivery times possible with our premium RAPID run services Strand specific mRNA sequencing protocols Special protocol for bacterial and fungal samples available Extensive data analysis options Sequencing at the lowest cost per basepair BaseClear is an independent service laboratory using ISO17025 and GMP compliant methods Many satisfied customers worldwide OPTIONAL BIOINFORMATICS Click here to read more about our RNA seq analysis possibilities Meet Adalberto Costessi Adalberto is manager of our Next Generation sequencing NGS department and is also

    Original URL path: http://www.baseclear.com/genomics/next-gen-sequencing/transcriptome-analysis (2016-02-12)
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  • Small-RNA sequencing - BaseClear
    disposables Lab equipment Microparticles Industries Industrial Biotechnology Food Ingredients Pharma Health Water Environment Clinical Diagnostics laboratories Agro Sciences Academic Research institutes About BaseClear Our company Meet our team History Partners Distributors Why BaseClear Reasons to work with BaseClear Research development Quality assurance Technologies Information News Ordering Career Jobs Internships Contact Contact details Contact form Request quotation Order forms Blog Home Our services Lab Products Industries About BaseClear Blog Next Gen sequencing Genome De Novo Re sequencing Transcriptome sequencing Small RNA sequencing Metagenomics Microbial profiling Bioinformatics Sanger sequencing Microbiology Synthetic Biology SMALL RNA SEQUENCING Our small RNA discovery and analysis service allows the discovery of rare small RNA without previous sequence or secondary structure information in any organism With this service we can find novel small RNA characterize mutations and analyse the differential expression of all small RNA in your sample simultaneously OPTIONAL BIOINFORMATICS Removal of small RNA adapter sequences Alignment of and annotation of small RNA sequences based on species specific datasets among which miRBase Ensemble Counting and clustering of annotated small RNA sequences The bioinformatics strategy will be determined in close collaboration between the customer and our bioinformatics specialist WHY CHOOSE BASECLEAR Experts in small RNA sequencing of bacteria

    Original URL path: http://www.baseclear.com/genomics/next-gen-sequencing/small-rna-sequencing (2016-02-12)
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  • Metagenome sequencing - BaseClear
    much attention in recent years Modern genomic technologies based on massive parallel DNA sequencing Next Generation Sequencing have opened a wide range of possibilities for genetic research on microbial genomes and communities BaseClear offers complete solutions to study the collective genomes of microorganisms present in a sample These studies are often referred to as metagenomics but also as environmental genomics ecogenomics or community genomics Genomic analysis can be applied to obtain information about the composition as well as the functional capabilities of an ecosystem This allows the study of functional genes and gene clusters as well as provide insights into the organism s phenotype This for example can lead to a better understanding and advances of food fermentation processes WHOLE GENOME SEQUENCING BASED METAGENOMICS ANALYSIS For 16S metagenomics please refer to our Microbial diversity analysis page Our whole genome sequencing WGS analysis protocol for metagenomics analysis generally involves sequencing on the Illumina MiSeq or HiSeq platform In brief paired end reads are assembled into metagenomes using an optimized in house protocol The resulting contigs are subject to so called genome binning which aims to reconstruct complete genomes as much complete as possible and assigns these to the closest possible taxonomy In addition a functional analysis is applied to detect ORFs and associated gene functions Also it is possible to use a reference guided approach thus not de novo which focuses on the presence or absence of genes genomes known prior to the start Please ask our Product specialist Metagenomics for the available options and example projects Our WGS based analysis protocol includes a sophisticated workflow comprising a metagenome assembly binning and taxonomic classification Delivered output De novo assembly of binned contigs Taxonomy table containing a summary contig annotations and their relative abundance Publication ready figures and plots barchart piechart cladogram

    Original URL path: http://www.baseclear.com/genomics/next-gen-sequencing/metagenomics (2016-02-12)
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