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  • Baseclear
    libraries Gene synthesis Custom cloning PCR cloning Mutagenesis Lab Products Zymo Research Epigenetics DNA purification RNA purification Other products Sample kits Epigenetic services Bio Basic Gene Synthesis Protein expression and purification Peptide synthesis Custom antibody service Kisker Biotech Lab disposables Lab equipment Microparticles Industries Industrial Biotechnology Food Ingredients Pharma Health Water Environment Clinical Diagnostics laboratories Agro Sciences Academic Research institutes About BaseClear Our company Meet our team History Partners Distributors Why BaseClear Reasons to work with BaseClear Research development Quality assurance Technologies Information News Ordering Career Jobs Internships Contact Contact details Contact form Request quotation Order forms Blog Home Our services Lab Products Industries About BaseClear Blog Our company Why BaseClear Reasons to work with BaseClear Research development Quality assurance Technologies Information Contact Back to overview ABI3730 XL BaseClear has over 20 years of experience in offering Sanger sequencing We have several ABI3730XL and ABI3730 sequencing platforms which are used for our Sanger sequencing but we also have dedicated machines for fragment analysis We offer all included high quality services Outsourcing Sanger sequencing will allow you to focus more efficiently on the core aspects of your research ADVANTAGES OF OUR SANGER SEQUENCING SERVICES Independent certified service laboratory GMP and ISO

    Original URL path: http://www.baseclear.com/why-baseclear/technologies/ABI3730%28XL%29_100_7.html (2016-02-12)
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  • Baseclear
    Bio Basic Gene Synthesis Protein expression and purification Peptide synthesis Custom antibody service Kisker Biotech Lab disposables Lab equipment Microparticles Industries Industrial Biotechnology Food Ingredients Pharma Health Water Environment Clinical Diagnostics laboratories Agro Sciences Academic Research institutes About BaseClear Our company Meet our team History Partners Distributors Why BaseClear Reasons to work with BaseClear Research development Quality assurance Technologies Information News Ordering Career Jobs Internships Contact Contact details Contact form Request quotation Order forms Blog Home Our services Lab Products Industries About BaseClear Blog Our company Why BaseClear Reasons to work with BaseClear Research development Quality assurance Technologies Information Contact Back to overview Vitek MS from BioMerieux MALDI TOF MALDI TOF identification using the VITEK MS from bioMérieux is achieved by obtaining spectra using MALDI TOF technology Matrix Assisted Laser Desorption Ionization Time of Flight and analyzing the spectra with the VITEK MS database The peaks from these spectra are compared to the characteristic pattern for a species genus of family of micro organism thus resulting in an identification BACTERIAL AND YEAST IDENTIFICATION USING MALDI TOF It is not possible to requests this service for mould samples only bacterial and yeast samples can be identified using the MALDI TOF If

    Original URL path: http://www.baseclear.com/why-baseclear/technologies/Vitek%C2%AE-MS-from-BioMerieux-%28MALDI-TOF%29_100_10.html (2016-02-12)
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  • Baseclear
    Reasons to work with BaseClear Research development Quality assurance Technologies Information News Ordering Career Jobs Internships Contact Contact details Contact form Request quotation Order forms Blog Home Our services Lab Products Industries About BaseClear Blog Our company Why BaseClear Reasons to work with BaseClear Research development Quality assurance Technologies Information Contact Back to overview MicroSEQ system Validated microbial identifications BACTERIAL IDENTIFICATIONS Identification of bacterial samples by PCR and DNA sequencing of a part of the 16S rRNA ribosomal gene using MicroSEQ validated protocols kits and database Primers bind to the conserved regions within this gene while the divergent regions are sequenced for identification The length of the consensus sequence is 475 bp long The analysis is based on the MicroSEQ system of Life Technologies This system includes validated kits and libraries containing entries for over 2200 bacterial species A full report including the homology search to the MicroSEQ validated database and sequence data will be mailed to the client within 2 4 working days for the standard service BC 304 or within one working day after we have received your sample s for our fast service BC 304F FUNGAL IDENTIFICATIONS Identification of fungal samples by PCR and DNA sequencing of

    Original URL path: http://www.baseclear.com/why-baseclear/technologies/MicroSEQ%C2%AE-system-Validated-microbial-identifications_100_9.html (2016-02-12)
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  • Baseclear
    cloning PCR cloning Mutagenesis Lab Products Zymo Research Epigenetics DNA purification RNA purification Other products Sample kits Epigenetic services Bio Basic Gene Synthesis Protein expression and purification Peptide synthesis Custom antibody service Kisker Biotech Lab disposables Lab equipment Microparticles Industries Industrial Biotechnology Food Ingredients Pharma Health Water Environment Clinical Diagnostics laboratories Agro Sciences Academic Research institutes About BaseClear Our company Meet our team History Partners Distributors Why BaseClear Reasons to work with BaseClear Research development Quality assurance Technologies Information News Ordering Career Jobs Internships Contact Contact details Contact form Request quotation Order forms Blog Home Our services Lab Products Industries About BaseClear Blog Our company Why BaseClear Reasons to work with BaseClear Research development Quality assurance Technologies Information Contact Back to overview Custom sequence identifications BaseClear offers complete identification services based on in house developed protocols Identification of bacterial or fungal samples is achieved by PCR and DNA sequencing of a part of customer requested genes e g 16S 28S rpoB gyrB ITS etc Primers bind to the conserved regions within the genes while the divergent regions are sequenced for identification The length of the consensus sequence is either 700 bp long BC 308 or 1400bp BC 309 For fungal

    Original URL path: http://www.baseclear.com/why-baseclear/technologies/Custom-sequence-identifications_100_13.html (2016-02-12)
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  • Baseclear
    Biotechnology Food Ingredients Pharma Health Water Environment Clinical Diagnostics laboratories Agro Sciences Academic Research institutes About BaseClear Our company Meet our team History Partners Distributors Why BaseClear Reasons to work with BaseClear Research development Quality assurance Technologies Information News Ordering Career Jobs Internships Contact Contact details Contact form Request quotation Order forms Blog Home Our services Lab Products Industries About BaseClear Blog Our company Why BaseClear Reasons to work with BaseClear Research development Quality assurance Technologies Information Contact Back to overview Microbial Typing using REP PCR To control unwanted bacteria you need to know what the contaminating organism is and how it entered your environment For this BaseClear offers a microbial characterization typing service using REP PCR in combination with DNA sequencing These techniques use the power of DNA to not only identify the genus species of contaminants but also provide deeper strain level typing at the same time This fine degree of resolution applies to environmental isolates pathogens spoilage organisms control strains beneficial organisms or any other bacteria that are important to the industry Quality control labs in for instance pharmaceutical biotechnological food and health care industries use these techniques to track and trend microbial contaminations Microbial isolates may

    Original URL path: http://www.baseclear.com/why-baseclear/technologies/Microbial-Typing-using-REP-PCR_100_8.html (2016-02-12)
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  • Baseclear
    History Partners Distributors Why BaseClear Reasons to work with BaseClear Research development Quality assurance Technologies Information News Ordering Career Jobs Internships Contact Contact details Contact form Request quotation Order forms Blog Home Our services Lab Products Industries About BaseClear Blog Our company Why BaseClear Reasons to work with BaseClear Research development Quality assurance Technologies Information Contact Back to overview Multilocus Sequence Typing MLST MLST is a technique in molecular biology for the typing of bacterial species The procedure characterizes isolates of bacterial species using the DNA sequences of internal fragments of multiple usually seven housekeeping genes Approximately 450 500 bp internal fragments of each gene are sequenced and compared to a public database for identification The great advantage of MLST is that sequence data are unambiguous and the allelic profiles of isolates can easily be compared to those in a large central database via the internet in contrast to most typing procedures which involve comparing fragment sizes on gels A further advantage of MLST is that the allegic profiles of isolates can be obtained from clinical material by PCR amplification of the seven house keeping loci directly from CSF or blood Thus isolates can be precisely characterised even when they

    Original URL path: http://www.baseclear.com/why-baseclear/technologies/Multilocus-Sequence-Typing-%28MLST%29_100_11.html (2016-02-12)
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  • Baseclear
    Research development Quality assurance Technologies Information Contact Back to overview Cell line authentication It is estimated that 15 20 of the human cell lines used in experiments have been misidentified or cross contaminated with another cell line More than 470 false continuous cell lines CCLs are known to exist and continue to be used in publications worldwide source http iclac org This poses a huge threat to the quality of publications For this reason depositories now demand cell line authentication reports with submissions of scientific publications ARE YOU USING THE CORRECT CELL LINE A cell line is misidentified if its DNA profile is no longer consistent with the donor from whom it was first established see also the database of cross contaminated and misidentified cell lines Investigators who are unaware that they are working with a false cell line may use it in their work which can lead to unreliable research findings and the use of those unreliable findings by other scientists in turn Cell lines are often handed from laboratory to laboratory without anyone being aware that their stocks may be unfit for use This practice can only lead to confusing results and represents a waste of precious resources INCORPORATE AUTHENTICATION TESTING INTO YOUR EVERYDAY CULTURE PRACTICE Authorities such as the ICLAC recommend to perform authentication of cell lines at the start of a research project and validate this periodically to track potential changes in cell lines Authentication testing is an effective way to combat the use of false cell lines Authentication testing aims to compare a test sample to other reference samples from that donor or to a database of reference samples if donor material is not available to see whether samples correspond RECOMMENDED TEST Baseclear offers several tests as recommended by ICLAC to verify your cell lines

    Original URL path: http://www.baseclear.com/why-baseclear/technologies/Cell-line-authentication_100_12.html (2016-02-12)
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  • Baseclear
    sequencing Mycoplasma detection Synthetic Biology Synthetic DNA libraries Gene synthesis Custom cloning PCR cloning Mutagenesis Lab Products Zymo Research Epigenetics DNA purification RNA purification Other products Sample kits Epigenetic services Bio Basic Gene Synthesis Protein expression and purification Peptide synthesis Custom antibody service Kisker Biotech Lab disposables Lab equipment Microparticles Industries Industrial Biotechnology Food Ingredients Pharma Health Water Environment Clinical Diagnostics laboratories Agro Sciences Academic Research institutes About BaseClear Our company Meet our team History Partners Distributors Why BaseClear Reasons to work with BaseClear Research development Quality assurance Technologies Information News Ordering Career Jobs Internships Contact Contact details Contact form Request quotation Order forms Blog Home Our services Lab Products Industries About BaseClear Blog Our company Why BaseClear Reasons to work with BaseClear Research development Quality assurance Technologies Information Contact Back to overview Genotyping services As huge amounts of DNA sequence data become available genomics research focuses more and more on studying genetic variations BaseClear is a frontrunner in the application of new genotyping techniques for purposes as diverse as human identification the search for mutations or studying the regulation of genes involved in major human diseases like cancer BaseClear offers a complete range of reliable high quality genotyping techniques

    Original URL path: http://www.baseclear.com/why-baseclear/technologies/Genotyping-services_100_14.html (2016-02-12)
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