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  • Sanger sequencing - BaseClear
    Sciences Academic Research institutes About BaseClear Our company Meet our team History Partners Distributors Why BaseClear Reasons to work with BaseClear Research development Quality assurance Technologies Information News Ordering Career Jobs Internships Contact Contact details Contact form Request quotation Order forms Blog Home Our services Lab Products Industries About BaseClear Blog Next Gen sequencing Bioinformatics Sanger sequencing Project sequencing Single runs 96 well plates Fragment analysis SNP and VNTR analysis Microbiology Synthetic Biology SANGER SEQUENCING SERVICES Sanger sequencing is based on sequencing on the ABI3730XL sequencer from Life Technologies BaseClear offers several services Project sequencing service An exclusive GMP compliant and all inclusive service that covers all steps necessary to determine the complete double or single stranded sequences of large DNA fragments Single run sequencing services BaseClear offers 4 distinct services for single run sequencing Full 24H sequencing Quick Shot sequencing prepaid BarCode sequencing and direct genomic sequencing sequence directly on genomic DNA from bacteria using Sanger sequencing eliminating the need for a PCR step in the process 96 well sequencing service High throughput DNA sequencing projects for low prices ADVANTAGES OF OUR SANGER SEQUENCING SERVICE Independent certified service laboratory GMP and ISO17025 compliant methods Highest quality data Excellent service

    Original URL path: http://www.baseclear.com/genomics/sanger-sequencing (2016-02-12)
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  • All inclusive Sanger sequencing service
    Peptide synthesis Custom antibody service Kisker Biotech Lab disposables Lab equipment Microparticles Industries Industrial Biotechnology Food Ingredients Pharma Health Water Environment Clinical Diagnostics laboratories Agro Sciences Academic Research institutes About BaseClear Our company Meet our team History Partners Distributors Why BaseClear Reasons to work with BaseClear Research development Quality assurance Technologies Information News Ordering Career Jobs Internships Contact Contact details Contact form Request quotation Order forms Blog Home Our services Lab Products Industries About BaseClear Blog Next Gen sequencing Bioinformatics Sanger sequencing Project sequencing Single runs 96 well plates Fragment analysis SNP and VNTR analysis Microbiology Synthetic Biology PROJECT SEQUENCING This exclusive service includes all steps necessary to determine the complete double or single stranded sequences of large DNA fragments A convenient option BaseClear s project sequencing service includes If necessary template preparation RT PCR and or purification of DNA samples DNA concentration measurement Design and synthesis of all necessary PCR and or sequencing primers Optimal sequencing reaction if necessary under specific conditions e g in case of GC rich DNA Assembly and manual editing of the sequence data into a contig sequence Comparison of sample contig sequence to a reference sequence if available Customer will be informed regularly about the progress Extensive advice for special projects Delivery time depends on read length and sequencing strategy Additional coverage possible upon request SINGLE OR DOUBLE STRANDED SEQUENCING Sequencing can be performed for one or for both DNA strands Double stranded DNA sequencing results in a higher accuracy of the sequence data If a reference sequence is known BaseClear can design the necessary primers on the basis of this PRIMER WALKING If a reference sequence is not available or if a clone contains an unknown insert the DNA can be sequenced via primer walking Due to the overlap of the resulting sequences

    Original URL path: http://www.baseclear.com/genomics/sanger-sequencing/project-sequencing (2016-02-12)
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  • Sanger sequencing - Single runs
    Academic Research institutes About BaseClear Our company Meet our team History Partners Distributors Why BaseClear Reasons to work with BaseClear Research development Quality assurance Technologies Information News Ordering Career Jobs Internships Contact Contact details Contact form Request quotation Order forms Blog Home Our services Lab Products Industries About BaseClear Blog Next Gen sequencing Bioinformatics Sanger sequencing Project sequencing Single runs 96 well plates Fragment analysis SNP and VNTR analysis Microbiology Synthetic Biology SINGLE RUN SEQUENCING BaseClear offers 4 different sequencing services for single run sequencing click on sequencing service for more detailed information Full 24H sequencing Extended and full service guaranteed quality and delivery time Quick Shot sequencing A convenient and flexible option at a competitive price for research purposes Prepaid BarCode sequencing Most cost efficient option for premix samples using prepaid BarCode labels Direct genomic sequencing Direct sequencing on bacterial genomic DNA See also our Standard primer list and Abbreviations Sanger sequencing FREE TRIAL FOR NEW CUSTOMERS For new customers BaseClear offers a free trial in order to get acquainted with our high quality sequencing service and customer support Outsourcing will allow you to focus more efficiently on the core aspects of your research BaseClear specializes in DNA sequencing so why won t you discover our extensive tailor made sequencing services with a free trial WHY CHOOSE BASECLEAR FOR SANGER SEQUENCING Sequencing experts since 1993 Fast delivery possible within 24 hour From single samples to large sample quantities for high throughput sequencing Automated and optimized protocols for constant and optimal results Various service options varying from a basic cost effective service to a high quality service for all types of samples Special protocols for complicated samples such as siRNA genomic DNA gDNA large DNA constructs such as BAC PAC cosmid lambda and GC rich DNA Additional possibilities for DNA

    Original URL path: http://www.baseclear.com/genomics/sanger-sequencing/single-runs (2016-02-12)
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  • Baseclear - 96-well plates
    well H12 empty this well will be used for internal sequence control Single sequencing reaction with a run time corresponding to either 1000 bp long run or 550 bp short run Sequencing data are automatically analysed by KB caller the international standard software for superior base calling and sequence data interpretation Sequence results are sent directly to the customer in FASTA SCF and ABI format Re runs are possible upon request Delivery time 1 2 working days Make sure that the plate is properly sealed and packaged to prevent leakage or damage during transport SAMPLE DELIVERY WHEN SAMPLE PLATE AND PRIMERS ARE SENT IN SEPARATE 96 well plate with bacteria either as glycerolstocks on dry ice or as cultures in LB medium 96 well plate with purified plasmid in a minimal volume of 20 ul per well with a DNA concentration of 50 200 ng ul Make sure the DNA concentrations are normalised over the plate 96 well plate with purified or Raw PCR product in a minimal volume of 20 ul per well with a DNA concentration 5 ng μl Make sure the DNA concentrations are normalised over the plate 2 μl of the PCR product on agarose gel should give a single clear and sharp band DNA preferably column purified dissolved in water or 10mM Tris pH8 0 Please note that DNA samples should be free of EDTA and ethanol since trace amounts of these compounds will inhibit the sequence reaction Primers You can choose a maximum of one primer per half plate which will be added by BaseClear If you want to use multiple primers per plate you can submit a mirror plate with the corresponding primers For a full 96 well plate please sent in 200 ul primer with a concentration of 10 pmol ul For

    Original URL path: http://www.baseclear.com/genomics/sanger-sequencing/96-well-plates (2016-02-12)
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  • Baseclear - Fragment analysis
    work with BaseClear Research development Quality assurance Technologies Information News Ordering Career Jobs Internships Contact Contact details Contact form Request quotation Order forms Blog Home Our services Lab Products Industries About BaseClear Blog Next Gen sequencing Bioinformatics Sanger sequencing Project sequencing Single runs 96 well plates Fragment analysis SNP and VNTR analysis Microbiology Synthetic Biology FRAGMENT ANALYSIS SERVICE Fragment analysis is used for a wide variety project involving genotyping DNA profiling and mutation analysis Applications include microsatellite analysis STR and VNTR AFLP T RLFP and SNP genotyping BaseClear offers a fast and reliable fragment analysis services We offer ready to run services size standard can be included by the customer or we can dilute your samples add a the size standard to your samples This service is performed on dedicated ABI3730 sequencers for accurate size calling ADVANTAGES OF OUR FRAGMENT ANALYSIS SERVICES Independent service laboratory using GMP and ISO17025 compliant methods Fast delivery of results usually 1 2 days Several dye sets calibrated Dedicate Applied Biosystems 3730 Genetic Analyzers Automated and optimized protocols for constant and optimal results Please contact one of our specialists to discuss your fragment analysis outsourcing wishes Meet Patrick Koning Patrick is account manager for mainly

    Original URL path: http://www.baseclear.com/genomics/sanger-sequencing/fragment-analysis (2016-02-12)
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  • SNP and VNTR analysis services
    sequencing Bioinformatics Sanger sequencing Project sequencing Single runs 96 well plates Fragment analysis SNP and VNTR analysis Microbiology Synthetic Biology SNP AND VNTR ANALYSIS Behavioural genetics examines the origin of variations in human behaviour More specifically it addresses to what extend variations in genetic disposition and environmental factors account for individual differences in mental and physical health and behaviour BaseClear offers a broad package of services for the analysis of genetic variations In recent years we have developed a range of genotyping tests that type polymorphisms in genes that are potentially involved in human behaviour Most of the genetic variation that is being used in behavioural genetics relates to either SNP s single nucleotide polymorphisms or VNTR variable number of tandem repeats BaseClear uses a combination of PCR DNA sequencing and DNA fragment analysis to very accurately type these mutations Full service including DNA purification in low and high throughput Samples may be delivered as purified DNA or as raw material either buccal swabs saliva or blood We can also provide buccal swabs for collection of samples together with collection protocols BaseClear has fully automated systems to isolate and quantify DNA from various sources in high throughput format and can perform the genetic testing of many samples per order Our possibilities include DRD2 SNP DRD4 receptor VNTR and SNP DAT1 VNTR 5HTT VNTR and SNP COMT SNP OXTR SNP GABA receptor SNP Is your gene of interest not listed We have many more assays and are also happy to design new genotyping tests for any gene of interested Depended on the expected number of samples design is usually done free of charge ORDERING IS EASY You can provide a number of different sample types including buccal swabs saliva samples e g oragene collection bottles blood or DNA Please always use

    Original URL path: http://www.baseclear.com/genomics/genotyping/snp-and-vntr-analysis (2016-02-12)
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  • Microbiology - BaseClear
    purification Other products Sample kits Epigenetic services Bio Basic Gene Synthesis Protein expression and purification Peptide synthesis Custom antibody service Kisker Biotech Lab disposables Lab equipment Microparticles Industries Industrial Biotechnology Food Ingredients Pharma Health Water Environment Clinical Diagnostics laboratories Agro Sciences Academic Research institutes About BaseClear Our company Meet our team History Partners Distributors Why BaseClear Reasons to work with BaseClear Research development Quality assurance Technologies Information News Ordering Career Jobs Internships Contact Contact details Contact form Request quotation Order forms Blog Home Our services Lab Products Industries About BaseClear Blog Next Gen sequencing Bioinformatics Sanger sequencing Microbiology Bacterial and fungal identifications Microbial typing Microbial diversity analysis Microbial genome sequencing Virus sequencing Mycoplasma detection Synthetic Biology MICROBIOLOGY Producers of biotechnological products need to assure that their products are of high quality and not contaminated with any type of micro organism or DNA This quality assurance has to be performed via accurate and reliable methods that are GMP and ISO compliant BaseClear offers several services for DNA based quality assurance using validated methods which are performed in our ISO17025 compliant laboratory One example of this is our validated MicroSEQ service for the fast identification of contaminating micro organisms to reveal their

    Original URL path: http://www.baseclear.com/microbiology (2016-02-12)
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  • Baseclear - Bacterial and fungal identifications
    services for fast and reliable identification of microorganisms BaseClear is specialized in fast identifications of micro organisms using molecular methods such as DNA sequencing MicroSEQ system or in house developed protocols or MALDI TOF technology using the Vitek MS from bioMérieux We have many satisfied customers working in for instance food water biotech and pharmaceutical industries BaseClear offers identification services using the revolutionary MicroSEQ system from Applied Biosystems for identification of microbiological samples based on PCR and DNA sequencing of the 16S rRNA for bacteria or the ITS or D2 LSU regions for fungal samples This system provides accurate and reproducible results without the need for time consuming growth It is suitable for fast screening 1 day for our fast service of even the most difficult samples In addition BaseClear offers identification services using the VITEK MS from BioMérieux By acquiring the VITEK MS we have expanded our microbial service package with a fast and reliable identification platform This proteotypic technology is 30 40 more accurate compared to existing phenotypic methods Additional advantages of this system are the delivery time possible within a couple of hours and the costs compared to other identification methods ADVANTAGES OF OUR MICROBIAL IDENTIFICATION SERVICES Several options available for bacterial or fungal identification Faster and more reliable than conventional identification methods 100 accurate and 100 reproducibility Contact our specialist to discuss which option best fits your needs Fast delivery within 24 hours possible Complete package of molecular methods for microbial identifications available Free trial available to get acquainted with our services Validated systems for identification of bacteria and fungi Complete report including genetic distance and phylogenic tree MicroSEQ system recommended by various agencies around the world including the US and European Pharmacopoeia Perfect solution for QA QC labs in food water pharmaceutical and public health

    Original URL path: http://www.baseclear.com/microbiology/bacterial-and-fungal-identifications (2016-02-12)
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