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  • Baseclear - Custom cloning
    service Kisker Biotech Lab disposables Lab equipment Microparticles Industries Industrial Biotechnology Food Ingredients Pharma Health Water Environment Clinical Diagnostics laboratories Agro Sciences Academic Research institutes About BaseClear Our company Meet our team History Partners Distributors Why BaseClear Reasons to work with BaseClear Research development Quality assurance Technologies Information News Ordering Career Jobs Internships Contact Contact details Contact form Request quotation Order forms Blog Home Our services Lab Products Industries About BaseClear Blog Next Gen sequencing Bioinformatics Sanger sequencing Microbiology Synthetic Biology Synthetic DNA libraries Gene synthesis Custom cloning PCR cloning Mutagenesis CUSTOM CLONING SERVICES Recombinant DNA cloning is not always easy to execute and often time consuming BaseClear has considerable experience in challenging cloning projects and can complete projects very efficiently Cloning projects can be complicated due to issues such as gene toxicity large vector or insert size unstable DNA elements or the presence of DNA secondary structures We have experience with many of these difficulties and can help you During the planning and execution of custom cloning projects we consult our customers and ensure that every aspect of the work is agreed upon before it is performed In addition to this we analyse every step of the cloning process This enables us to assess the success of every stage FAST CLONING SERVICE We can offer a fast cloning service with a delivery time for cloning projects as fast as 7 days This service will help customers to speed up their work and gives the possibility to obtain the construct you need within a short time and without spending valuable time on routine cloning work Meet Evelien Zeinstra Evelien is one our dedicated account managers She regularly visits customers and gives presentations at exhibitions and conferences Her main focus is the sales of next generation sequencing services Testimonial University of

    Original URL path: http://www.baseclear.com/synthetic-biology/molecular-biology/custom-cloning (2016-02-12)
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  • PCR cloning - BaseClear
    institutes About BaseClear Our company Meet our team History Partners Distributors Why BaseClear Reasons to work with BaseClear Research development Quality assurance Technologies Information News Ordering Career Jobs Internships Contact Contact details Contact form Request quotation Order forms Blog Home Our services Lab Products Industries About BaseClear Blog Next Gen sequencing Bioinformatics Sanger sequencing Microbiology Synthetic Biology Synthetic DNA libraries Gene synthesis Custom cloning PCR cloning Mutagenesis PCR CLONING BaseClear offers an attractively priced standard PCR cloning service For a fixed price we will execute your PCR cloning project and verify the results with DNA sequencing all within 10 working days Our PCR cloning service in short Send in a minimum of 100 ng of PCR products containing one specific band and include a gel picture OR send in a minimum of 50 uL raw PCR product Maximum length of PCR products is 1500 bp For efficient cloning PCR products need to be 3 adenylated and purified Please indicate on the sample sheet whether we need to perform these additional steps for you Positive clones containing inserts of the indicated length will be identified by colony PCR and inserts of 3 clones will be verified by sequencing using vectorprimers When available the sequences will be aligned to a reference sequence BaseClear guarantees the complete confidentiality of all client information project data and biological samples during or after the termination of the project In case of gene toxicity the client is olbiged to pay the full price Gene toxicity will be determined by comparing ligation efficiency and transformation efficiency Optional possibilities PCR product purification A tailing A small aliquot of the isolated clones can be sent back to the customer as purified DNA Meet Danny Duijsings Danny is our R D director and manages the synthetic biology department Danny has a

    Original URL path: http://www.baseclear.com/synthetic-biology/molecular-biology/pcr-cloning (2016-02-12)
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  • Mutagenesis -BaseClear
    Clinical Diagnostics laboratories Agro Sciences Academic Research institutes About BaseClear Our company Meet our team History Partners Distributors Why BaseClear Reasons to work with BaseClear Research development Quality assurance Technologies Information News Ordering Career Jobs Internships Contact Contact details Contact form Request quotation Order forms Blog Home Our services Lab Products Industries About BaseClear Blog Next Gen sequencing Bioinformatics Sanger sequencing Microbiology Synthetic Biology Synthetic DNA libraries Gene synthesis Custom cloning PCR cloning Mutagenesis MUTAGENESIS Based on optimised protocols BaseClear is able to create mutations in virtually every DNA fragment This is a valuable tool for studying DNA or protein structure and function Site directed or random mutagenesis can be applied to any DNA fragment cloned into a plasmid vector We can help you to introduce a range of mutations varying from point mutations to deletions or insertions The final construct will be delivered as purified plasmid DNA of your vector with the mutation in combination with an E coli stab containing this plasmid The samples will be accompanied by a report of the mutagenesis project including the raw sequencing data and a complete alignment of the sequence 500 bp around the mutation both electronic Meet Björn te Boekhorst In

    Original URL path: http://www.baseclear.com/synthetic-biology/molecular-biology/mutagenesis (2016-02-12)
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  • Baseclear - Lab Products
    Food Ingredients Pharma Health Water Environment Clinical Diagnostics laboratories Agro Sciences Academic Research institutes About BaseClear Our company Meet our team History Partners Distributors Why BaseClear Reasons to work with BaseClear Research development Quality assurance Technologies Information News Ordering Career Jobs Internships Contact Contact details Contact form Request quotation Order forms Blog Home Our services Lab Products Industries About BaseClear Blog Zymo Research Bio Basic Kisker Biotech LAB PRODUCTS DISTRIBUTED BY BASECLEAR We offer several high quality lab products from different suppliers ZYMO RESEARCH BaseClear offers innovative products from Zymo Research for Epigenetics DNA RNA isolation and purification and several other molecular biological lab products All Zymo Research products can be characterised as simple and reliable products for DNA research BaseClear is the official and exclusive distributor of Zymo Research products within The Netherlands BIO BASIC Bio Basic products include gene synthesis protein expression and purification peptide synthesis and a custom antibody service These services are marketed at very affordable rates BaseClear is the official distributor of Bio Basic services within The Netherlands Belgium Italy and Scandinavia Denmark Finland Norway Iceland Sweden KISKER BIOTECH Via Kisker Biotech we offer products like molecular biological lab equipment such as pipettes electrophoresis units

    Original URL path: http://www.baseclear.com/lab-products (2016-02-12)
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  • Baseclear - Zymo Research
    services Bio Basic Gene Synthesis Protein expression and purification Peptide synthesis Custom antibody service Kisker Biotech Lab disposables Lab equipment Microparticles Industries Industrial Biotechnology Food Ingredients Pharma Health Water Environment Clinical Diagnostics laboratories Agro Sciences Academic Research institutes About BaseClear Our company Meet our team History Partners Distributors Why BaseClear Reasons to work with BaseClear Research development Quality assurance Technologies Information News Ordering Career Jobs Internships Contact Contact details Contact form Request quotation Order forms Blog Home Our services Lab Products Industries About BaseClear Blog Zymo Research Epigenetics DNA purification RNA purification Other products Sample kits Epigenetic services Bio Basic Kisker Biotech ZYMO RESEARCH LAB PRODUCTS BaseClear offers innovative products from Zymo Research for Epigenetics DNA RNA purification and several other molecular biological lab products All Zymo Research products can be characterised as simple and reliable products for DNA research For the complete overview of products please visit www zymoresearch com For pricing please inquire via our contact form FREE SAMPLE KITS FOR EVALUATION As we are convinced that these products will meet your requirements we offer free sample sized kits for the most popular DNA RNA isolation kits and DNA methylation kits to experience the quality and easy of

    Original URL path: http://www.baseclear.com/lab-products/zymo-research (2016-02-12)
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  • Baseclear - Epigenetics
    About BaseClear Blog Zymo Research Epigenetics DNA purification RNA purification Other products Sample kits Epigenetic services Bio Basic Kisker Biotech EPIGENETICS Epigenetic regulation refers to biological mechanisms in which DNA RNA and proteins are chemically or structurally modified without changing their primary sequence These epigenetic modifications play critical roles in the regulation of numerous cellular processes including gene expression DNA replication and recombination Epigenetic regulatory mechanisms include DNA methylation and hydroxymethylation histone modification chromatin remodeling RNA methylation and regulation by small and long non coding RNAs While epigenetic modifications can be very stable and passed on to multiple generations in some cases they can also dynamically change in response to specific cellular conditions or environmental stimuli When epigenetic mechanisms are misregulated the result can be detrimental to health and can lead to cancer neurological disorders and developmental abnormalities Therefore epigenetic modifications are emerging as important diagnostic and prognostic biomarkers in many fields of medicine In recent years epigenetics has exploded into one of the most exciting and rapidly expanding fields in biology Zymo Research has grown with it becoming The Epigenetics Company Zymo Research understands the need for high quality and reliable products for epigenetics research and we offer an extensive and continually growing line of products kits and genome wide services to facilitate investigations into epigenetic regulation of cellular processes We are committed to continue meeting the needs of epigenetic researchers into the future PRODUCTS CATEGORIES DNA METHYLATION DNA methylation remains one of the most extensively studied areas of epigenetic research and Zymo Research s products for investigating DNA methylation are the most cited and trusted in the industry DNA HYDROXYMETHYLATION DNA hydroxymethylation is an epigenetic modification that is being increasingly investigated yet its biological remains unclear RNA METHYLATION Zymo Research assists scientists in pioneering the emerging field of

    Original URL path: http://www.baseclear.com/lab-products/zymo-research/epigenetics (2016-02-12)
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  • Baseclear - DNA purification
    PCR DNA ligation cloning sequencing arrays etc The scientists at Zymo Research have developed the most comprehensive technologies for DNA clean up and concentration from any preparation Central to these products is the total removal of salts and alcohol from samples through the use of uniquely designed spin columns and plates that ensure complete elution of DNA without binding wash buffer carryover Coupled with uniquely formulated buffers these DNA clean up technologies assure the purification of high quality DNA with the complete removal of enzymatic inhibitors Plasmid DNA Purification Overview Plasmid DNA purification is a cornerstone of many molecular biology and biochemistry labs due to its extensive use prior to a variety of applications such as transfection bacterial transformation restriction endonuclease digestion PCR sequencing and in vitro transcription Therefore kits that can robustly isolate high quality endotoxin free plasmid DNA are indispensable tools in every researcher s arsenal Zymo Research provides unrivaled plasmid DNA purification kits at an affordable price so that every lab can be equipped with the essentials Several formats are available for isolating bacterial plasmids yeast plasmids or BAC YAC PAC DNA High quality plasmids isolated using our technology can be eluted directly from the column in water the ultra pure DNA is ready for all sensitive downstream applications Genomic DNA Overview Zymo Research offers a range of genomic DNA isolation kits that are suitable for isolating DNA from a wide variety of sample types including tissue fresh and paraffin embedded tissue sections cultured cells saliva buccal cells whole blood plasma serum urine bacteria fungi yeast algae viruses and mitochondria These isolation kits yield high quality dsDNA that is ideal for use in downstream applications such as PCR Southern blotting endonuclease digestion and methylation detection Similar to the DNA clean up series of kits the genomic DNA isolation kits feature Zymo Spin technology for complete inhibitor and contaminant removal minimal elution volumes and high concentrations of recovered DNA Microbial Environmental DNA Isolation Overview Bead beating is often required for the efficient processing of tough to lyse organisms and environmental samples and the environmental DNA isolation kits from Zymo Research feature unique BashingBead technology for efficient isolation of DNA from samples otherwise refractory to conventional lysis procedures State of the art BashingBeads are constructed of the highest quality most dense ceramic material available today They are used when thorough sample homogenization lysis is required by the researcher and DNA shearing by physical and chemical methods is minimized since the beads are fracture resistant and chemically inert respectively They are unique amongst the lysis matrices offered by other companies for DNA isolation from tough to lyse materials The BashingBead kits from Zymo research can be effectively used for processing tough to lyse tissues soil samples feces plants seeds food arthropods gram and gram bacteria yeast filamentous fungi unicellular and filamentous algae and protozoa For processing samples are simply transferred to the provided ZR BashingBead Lysis Tubes where samples are rapidly and efficiently lysed by bead beating in uniquely designed

    Original URL path: http://www.baseclear.com/lab-products/zymo-research/dna-purification (2016-02-12)
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  • Baseclear - RNA purification
    for the selective enrichment of either small RNA 200 nucleotides large RNA 200 nucleotides or both small and large RNAs separately in different fractions at the discretion of the user RNA TECHNOLOGIES RNA Clean up RNA may need to be cleaned up to remove enzymes buffers or chemical inhibitors and concentrated for use in certain applications With the RNA clean up kits from Zymo Research hiqh quality RNA from pg to 100 g amounts can be purified and concentrated with ultra pure RNA eluted in as little as 6 l RNA Isolation Depending on the sample type and downstream application Zymo Research provides a variety of ribonucleic acid RNA purification kits for the isolation of high quality DNA free RNA Whether it s total RNA including small RNAs separate fractions of RNA large RNA 200 nt or small RNAs 17 200 nt or RNA and DNA from the same sample there is a technology specifically designed for the application Researchers can isolate RNA from a wide variety of sample types including fresh frozen cells and tissues FFPE tissues whole blood plasma serum urine yeast bacteria plant tissue and more The isolation kits can accommodate down to picogram amounts of RNA single cell with innovative Zymo Spin column plate and magnetic bead technologies DNA Free RNA For many downstream applications it is essential to have RNA that is free of contaminating genomic DNA Zymo Research s RNA isolation kits remove the vast majority of genomic DNA and feature convenient in column DNase I treatment The DNA Free RNA Kit Direct zol RNA and Quick RNA kits include DNase I for fast and efficient genomic DNA removal Small microRNA Isolation The importance of miRNAs and other small RNAs in regulating gene expression and other cellular processes is clear but many kits from other companies do not effectively recover miRNAs and other small RNA molecules In addition many small RNAs are expressed at low abundance or only in specific cell types To obtain results you can trust you need to recover ALL small RNAs during your purification The RNA isolation kits from Zymo Research efficiently recover small RNA molecules as short as 17 nucleotides DNA RNA Co Purification RNA and DNA can be isolated simultaneously or into two separate fractions Purify up to 25 g DNA and or 50 g RNA with DNA RNA co purification and parallel purification kits from Zymo Research RNA Ladders The ZR small RNA Ladder consists of four single stranded RNA oligonucleotides 17 21 25 and 29 bases in length and is suitable as a molecular weight marker for microRNAs in polyacrylamide gel electrophoresis PAGE experiments The RNA ladder is supplied in water and can be stained with dyes specific for single stranded nucleic acid species e g GelStar Enzymes In many cases high quality enzymes are needed to increase the efficiency of RNA purification methods DNase I is suited for applications including removal of DNA template after in vitro transcription and removal of DNA from RNA samples

    Original URL path: http://www.baseclear.com/lab-products/zymo-research/rna-purification (2016-02-12)
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