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  • Baseclear - Other products
    About BaseClear Our company Meet our team History Partners Distributors Why BaseClear Reasons to work with BaseClear Research development Quality assurance Technologies Information News Ordering Career Jobs Internships Contact Contact details Contact form Request quotation Order forms Blog Home Our services Lab Products Industries About BaseClear Blog Zymo Research Epigenetics DNA purification RNA purification Other products Sample kits Epigenetic services Bio Basic Kisker Biotech ZYMO RESEARCH OTHER PRODUCTS COMPETENT CELLS Ready to use competent E coli cells and materials to prepare your own competent cells Zymo Research provides a simple method for generating Z competent E coli for rapid reliable and highly efficient DNA transformation Z competent cells can be transformed in seconds Mix Go without heat shocking lengthy incubations or outgrowth steps ANTIBIOTICS AND CHEMICALS A range of pre made high quality antibiotics and chemicals Zymo Research ready to use ampicillin chloramphenicol kanamycin and tetracycline are perfect for use in bacterial selection procedures PRODUCTS FOR YEAST RESEARCH Zymo Research offers a range of convenient and innovative products for yeast research We provide a uniquely formulated YPD plus medium that increases the transformation efficiency of most yeast strains by 50 The frozen EZ Yeast Transformation Kit has been designed to

    Original URL path: http://www.baseclear.com/lab-products/zymo-research/other-products (2016-02-12)
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  • Baseclear - Sample kits
    Genomic DNA Clean Concentrator 10 5 preps The Genomic DNA Clean Concentrator DCC is for the quick 5 minute recovery of ultra pure large sized DNA e g genomic mitochondrial plasmid BAC PAC viral phage wga DNA etc from any enzymatic reaction or impure preparation e g Proteinase K digestion D4036S Zyppy Plasmid miniprep 10 preps The Zyppy can be used for isolation of transfection quality plasmid DNA directly from culture without the need to spin down the bacterial cells its pellet free D5005S EZ DNA Methylation Gold 10 rxns From the family of most cited bisulfite treatment technologies the Gold kit can be used for complete conversion of DNA in less than 3 hours for reliable DNA methylation analysis D5020S EZ DNA Methylation Direct 10 rxns Cells and tissue e g FFPE can be input directly into this bisulfite treatment kit There is no prerequisite for DNA purification Compatible with low sample inputs ie 10 cells D5030S EZ DNA Methlyation lightning 10 rxns The fastest method for complete bisulfite conversion of DNA for methylation analysis Pre made conversion reagent facilitates rapid scale up for high throughput and automated applications D6030S OneStep PCR Inhibitor Removal 5 preps One step complete removal of polyphenolic PCR inhibitors from impure DNA RNA preparations R1015S RNA Clean Concentrator 5 5 preps The RCC is for rapid cleanup of RNA from any enzymatic reaction The unique spin column facilitates microelution of RNA in 6 l R1054S Quick RNA MiniPrep 5 preps Quick easy isolation of small and large RNAs from a variety of cells and soft tissue samples in 10 minutes DNase I provided for convenient in column digestion R2050S Direct zol RNA MiniPrep 10 Preps Isolate small and large RNAs Directly from TRIzol or TRI Reagent without phase separation in 10 minutes DNase I

    Original URL path: http://www.baseclear.com/lab-products/zymo-research/sample-kits (2016-02-12)
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  • Baseclear - Epigenetic services
    Industrial Biotechnology Food Ingredients Pharma Health Water Environment Clinical Diagnostics laboratories Agro Sciences Academic Research institutes About BaseClear Our company Meet our team History Partners Distributors Why BaseClear Reasons to work with BaseClear Research development Quality assurance Technologies Information News Ordering Career Jobs Internships Contact Contact details Contact form Request quotation Order forms Blog Home Our services Lab Products Industries About BaseClear Blog Zymo Research Epigenetics DNA purification RNA purification Other products Sample kits Epigenetic services Bio Basic Kisker Biotech EPIGENETIC SERVICES It is now well established that the classic genetic code is not sufficient to explain many complex phenotypes and causes of human diseases Epigenetic modifications to DNA such as methylcytosine 5 mC and hydroxymethylcytosine 5 hmC must also be considered as they can have profound effects on gene expression and are important for nearly every aspect of biology These modifications can be stable inheritable and passed on to future generations With recent advances in sample preparation high throughput sequencing and bioinformatics researchers looking to study DNA methylation and hydroxymethylation now have several options for investigating these epigenetic modifications on a genome wide scale BaseClear and Zymo Research offers a comprehensive suite of epigenetic services including multiple platforms for

    Original URL path: http://www.baseclear.com/lab-products/zymo-research/epigenetic-services (2016-02-12)
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  • Baseclear - Bio Basic
    services Bio Basic Gene Synthesis Protein expression and purification Peptide synthesis Custom antibody service Kisker Biotech Lab disposables Lab equipment Microparticles Industries Industrial Biotechnology Food Ingredients Pharma Health Water Environment Clinical Diagnostics laboratories Agro Sciences Academic Research institutes About BaseClear Our company Meet our team History Partners Distributors Why BaseClear Reasons to work with BaseClear Research development Quality assurance Technologies Information News Ordering Career Jobs Internships Contact Contact details Contact form Request quotation Order forms Blog Home Our services Lab Products Industries About BaseClear Blog Zymo Research Bio Basic Gene Synthesis Protein expression and purification Peptide synthesis Custom antibody service Kisker Biotech BIO BASIC Bio Basic Group is a privately owned dynamic biotechnology company The company has a wide variety of Life Science Products available to better serve researchers in the field of life sciences Bio Basic has many satisfied clients from leading biotechnology and pharmaceutical industries to highly accredited academic institutions worldwide BaseClear is the official distributor of Bio Basic services within The Netherlands Belgium Italy and Scandinavia Denmark Finland Norway Iceland Sweden Bio Basic Group is committed to innovation and excellence Throughout its development Bio Basic Group has continuously directed efforts into R D and improving the quality

    Original URL path: http://www.baseclear.com/lab-products/bio-basic (2016-02-12)
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  • Baseclear - Gene Synthesis
    accredited academic institutions worldwide BaseClear is the official distributor of Bio Basic services within The Netherlands Belgium Italy and Scandinavia Denmark Finland Norway Iceland Sweden GUARANTEED LOWEST PRICE As many budgets have been cut down Bio Basic has decided to guarantee to beat any of their competitors pricing on gene synthesis by 10 The genes they deliver are fully cloned and 100 sequence verified Codon optimization is included in the standard prices Genes are cloned into standard pUC57 Amp Ampicillin treated or pUC57 kan Kanamycin treated vectors Should you require to clone the gene into a vector other than pUC57 or pBluescript there is an additional fee DELIVERABLES You will receive 2 4ug of lyophilized DNA containing synthetic gene of interest sequence chromatogram trace files gene report QC restriction digestion files an alignment file as well as vector information If you wish to receive up to 10ug of plasmid DNA we are pleased to provide it to you at no extra cost However an additional turn around time would be required Further discounts on large projects and long term contracts are also available Discount rates are based on total number of base pairs of your gene order Single stranded sequencing only Double stranded sequencing service is available upon request Terms Conditions apply check with your BaseClear account manager GUARANTEED COMMITMENT Bio Basic is approved for ISO9001 2000 Bio Basic offers a 100 correct DNA Sequencing guarantee each gene is confirmed base by base by sequence and is guaranteed to match 100 with your requested sequences Quick Turnaround Lowest price guaranteed Absolute confidentiality guarantee Lowest price guaranteed Bio Basic will match any competitor s advertised price for identical products or services offered in the Bio Basic Canada Inc catalogues and on www biobasic com within 30 days Bio Basic reserves the

    Original URL path: http://www.baseclear.com/lab-products/bio-basic/gene-synthesis (2016-02-12)
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  • Baseclear - Protein expression and purification
    Prokaryotic recombinant protein expression systems E Coli have several advantages including ease of culture rapid cell growth and relatively simple purification procedures However most post translational modifications are not added by bacteria and most proteins become insoluble in inclusion bodies and are very difficult to recover as functional proteins Eukaryotic systems for the expression of protein include yeast baculovirus cells and mammalian cells Advantages of these systems include high levels of expression no inclusion bodies and intact post translational modifications The expression systems we offer include E Coli Expression System Escherichia coli E coli is one of the most widely used hosts for the production of heterologous proteins Its genetics are far better characterized than those of any other microorganism Baculovirus Expression System The Baculovirus insect cell expression system is widely used for the production of precisely matured folded and processed recombinant proteins In contrast to E coli insect cells are able to incorporate post translational modifications This unique tool usually yields high amounts of the target protein making it highly cost effective in comparison to other eukaryotic expression systems Mammalian Expression System Mammalian cell lines CHO HEK 293 etc are widely used for the production of recombinant glycoproteins vaccines enzymes as well as hormones and immunobiologicals antibodies interleukins lymphokines as they have the advantage of full posttranslational modifications of the expressed protein Yeast expression system Available upon special request For E Coli expression projects there are standard prices and they come in guaranteed packages of 1 mg 5 mg and 15 mg of purified protein In the case of the Baculovirus or Mammalian expression projects we start with a pilot study please inquire about pricing in order to analyze whether the expression would be high or low Based on the results of the pilot study the pricing for the subsequent full length project will be determined After the pilot study you can choose whether to continue with the project should you be satisfied with the price and pilot study results Projects can either be initiated from gene synthesis or you can simply provide the constructs that you would like to express At the end of the project we deliver all of the products we guarantee amount of protein or study results THINGS TO CONSIDER FOR YOUR PROJECT Sequence Optimization In highly expressed genes a narrow set of codons is used and these codons correspond to the more abundant tRNA species This minimizes the risk of tRNA depletion during translation In fact the codons in a gene may be true bottlenecks especially in cases where foreign genes are expressed in a host in which the usage of codons in highly expressed genes does not resemble the usage of codons in the species from which the foreign gene originates In such cases it has been shown that substitution of rare codons in the introduced gene may increase the yield dramatically In addition replacement of rare codons might decrease the chance of misincorporation and protect the protein from premature turnover Expression System

    Original URL path: http://www.baseclear.com/lab-products/bio-basic/protein-expression-and-purification (2016-02-12)
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  • Baseclear - Peptide synthesis
    team History Partners Distributors Why BaseClear Reasons to work with BaseClear Research development Quality assurance Technologies Information News Ordering Career Jobs Internships Contact Contact details Contact form Request quotation Order forms Blog Home Our services Lab Products Industries About BaseClear Blog Zymo Research Bio Basic Gene Synthesis Protein expression and purification Peptide synthesis Custom antibody service Kisker Biotech PEPTIDE SYNTHESIS SERVICE BaseClear and Bio Basic offer a high quality peptide synthesis service Our protocols QA QC procedures and instrumentations assure that quality products are synthesized and delivered Bio Basic has many satisfied clients from leading biotechnology and pharmaceutical industries to highly accredited academic institutions worldwide BaseClear is the official distributor of Bio Basic services within The Netherlands Belgium Italy and Scandinavia Denmark Finland Norway Iceland Sweden KEY FEATURES Years of experience in Peptide Synthesis From small peptides to long peptides 100 amino acids From the regular peptides to all kinds of modified peptides From mgs to kilograms GMP standard peptides are available All peptides are delivered with HPLC and Mass Spectroscopy data Fast turnaround time Low prices SPECIFICATIONS All custom peptides are supplied with strict analytical specifications which include HPLC and MS analysis Most standard custom peptides can be delivered

    Original URL path: http://www.baseclear.com/lab-products/bio-basic/peptide-synthesis (2016-02-12)
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  • Baseclear - Custom antibody service
    Research institutes About BaseClear Our company Meet our team History Partners Distributors Why BaseClear Reasons to work with BaseClear Research development Quality assurance Technologies Information News Ordering Career Jobs Internships Contact Contact details Contact form Request quotation Order forms Blog Home Our services Lab Products Industries About BaseClear Blog Zymo Research Bio Basic Gene Synthesis Protein expression and purification Peptide synthesis Custom antibody service Kisker Biotech CUSTOM ANTIBODY SERVICE With our partner Bio Basic we can now offer a Custom Antibody service Whether you desire antibodies for Western Blot or Immunohistochemistry ELISA or ambitious projects that are highly specialized to your lab Bio Basic can help you obtain the results that you need Their packages are structured and give you the freedom to synthesize from cDNA peptide or protein an antibody that will be successful in basic applications or guaranteed to work in your assay of choice Our qualified staff and experienced scientists can help you choose the Custom Antibody package that suits your project goals We will keep you informed throughout the generation of custom antibodies Contact info baseclear com to request your quote Our goal is to deliver superior results in the most cost effective manner FEATURES ELISA

    Original URL path: http://www.baseclear.com/lab-products/bio-basic/custom-antibody-service (2016-02-12)
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