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  • Biological effect profiling - Biodetection Systems
    and certification Biological effect profiling BDS offers an extensive panel of CALUX reporter assays that allow for identification of more than 30 distinct biological effects that can be evoked by a wide range of compound classes and mixtures These effects are related to adverse health effects such as endocrine disruption acute stress genotoxicity carcinogenicity obesity xenobiotic metabolism and reproductive toxicity In addition assays can also be used to identify compounds that have beneficial health effects e g through prevention of activation of the adverse effect pathways The majority of effects of chemical compounds on the human body take place or have an onset at the molecular level BDS has developed a wide range of assays that measure the effect of chemicals or chemical mixtures in modulating such critical cellular pathways Effect profiling with the CALUX panel can be applied for among others product safety assessment chemical safety assessment and environmental hazard identification All CALUX assays that are currently available are presented here Due to the standardized setup of our panel and the use of our high throughput facilities most effect profiles can be performed in a cost efficient manner Important benefits of biological effect profiling using CALUX assays are CALUX

    Original URL path: http://www.biodetectionsystems.com/services/bioanalyses/biological-effect-profiling.html (2016-02-10)
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  • Contaminant detection and quantification - Biodetection Systems
    ISO 17025 accredited for analysis of dioxins furans and dioxin like compounds non dioxin like PCBs and estrogenic compounds Dioxins furans and dioxin like compounds Food and feed safety is a high priority issue for the food and feed sector as it directly impacts human and animal health Stringent EU limit values are in force for dioxins in food and feeding stuffs for animal and public health protection Commission Regulation EU No 589 2014 food and Commission Regulation EU No 278 2012 feed The use of the DR CALUX bioassay for monitoring dioxins in food and feed allows the pre selection of samples suspected of being contaminated above limit values with dioxins Non dioxin like PCBs Non dioxin like PCBs are also regulated for food and feed samples according to the above mentioned EU regulations In contrast to dioxins and dioxin like chemicals non dioxin like PCBs are analyzed using chemical analytical methods GC ECD This method is also ISO 17025 accredited Polycyclic Aromatic Hydrocarbons PAHs Polycyclic aromatic hydrocarbons are typically present in matrices as complex mixtures The PAH CALUX detects in particular the toxic PAHs and is therefore perfectly suitable for hazard identification Estrogenic compounds The detection and quantification of estrogenic compounds in e g environmental water samples by CALUX analysis is also ISO 17025 accredited Environmental estrogens are contaminants that can mimic the biological effects of the female hormone estrogen in the endocrine system Xenoestrogens and phytoestrogens act as so called Endocrine Disrupting Compounds EDCs Other endocrine disruptive compounds In addition to our CALUX assay for estrogenic compound detection CALUX assays are available for compounds that disrupt the function of other natural hormones such as androgens progestins glucocorticoids retinoic acids thyroid hormones and peroxisome proliferator receptor interacting compounds An overview of the respective assays is available here Genotoxic

    Original URL path: http://www.biodetectionsystems.com/services/bioanalyses/contaminant-detection-and-quantification.html (2016-02-10)
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  • Assay principle - Biodetection Systems
    Print Bioassays Assay principle Assay procedure Service analysis and licensing Available assays Reference materials Standards Tissue culture products Extraction clean up products Assay principle Mammalian cells possess a wide range of mechanisms with which they can specifically respond to signals or stresses evoked by compounds they are confronted with CALUX Chemically Activated LUciferase eXpression assays form a panel of mammalian cell lines that were modified to produce a quantifiable response in addition to this natural response If a cell is triggered to generate a response a reporter gene luciferase is transcribed and translated into an enzyme that produces light during a reaction it catalyses After addition of a substrate luciferine light production is quantified using a luminometer The produced signal is proportional to the evoked effect An overview of the CALUX assays currently available is presented here Most cellular responses are mediated by a regulatory protein which is a sensor that detects a certain event and subsequently incites the cellular response to this event The regulator binds to a specific regulatory element which is present in DNA regions upstream of genes involved in the response In the CALUX cell lines these regulatory elements were also placed in front of the

    Original URL path: http://www.biodetectionsystems.com/products/bioassays/assay-principle.html (2016-02-10)
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  • Assay procedure - Biodetection Systems
    materials Standards Tissue culture products Extraction clean up products Assay procedure CALUX assays may be used for testing of compounds as well as complex mixtures The cells are cultured in well plates usually 96 or 384 wells which allows for increased throughput and automated handling Subsequently they are exposed to a dilution series of the test compound or a dilution series of an extract of the test sample Along with

    Original URL path: http://www.biodetectionsystems.com/products/bioassays/assay-procedure.html (2016-02-10)
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  • Service analysis and licensing - Biodetection Systems
    Water quality Innovations Expertise Facilities Projects Contract research News Downloads News Events Publications Downloads Contact Contact information Directions International partners Login Print Bioassays Assay principle Assay procedure Service analysis and licensing Available assays Reference materials Standards Tissue culture products Extraction clean up products Service analysis and licensing We run all CALUX assays for a wide range of applications and matrices in our service laboratory Moreover CALUX assays can be run

    Original URL path: http://www.biodetectionsystems.com/products/bioassays/service-analysis-and-licensing.html (2016-02-10)
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  • Available assays - Biodetection Systems
    Table 1 Overview of HTS CALUX panel Name Pathway Effect Reference Compound Key Reference DR CALUX Xenobiotics metabolism dioxin receptor activation 2 3 7 8 TCDD Aarts et al 1995 PAH CALUX Xenobiotics metabolism dioxin receptor activation benzoapyrene Pieterse et al 2013 ER CALUX Estrogen signaling 17β estradiol Legler et al 1999 ERα CALUX Estrogen receptor α mediated signaling 17β estradiol Sonneveld et al 2005 OECD unpublished ERα anti CALUX Repression of estrogen receptor α mediated signaling tamoxifen Van der Burg et al 2010a OECD unpublished ERβ CALUX Estrogen receptor β mediated signaling 17β estradiol Van der Burg et al 2013 ERβ anti CALUX Repression of estrogen receptor β mediated signaling tamoxifen AR CALUX Androgen receptor activation dihydrotestosterone Sonneveld et al 2005 OECD unpublished AR anti CALUX Repression androgen receptor activation flutamide Van der Burg et al 2010b OECD unpublished PR CALUX Progesterone receptor mediated signaling progesterone Sonneveld et al 2005 PR anti CALUX Repression of progesterone receptor mediated signaling RU486 GR CALUX Glucocorticoid receptor mediated signaling dexamethasone Sonneveld et al 2005 anti GR CALUX Repression of glucocorticoid receptor mediated signaling RU486 TR CALUX Thyroid receptor mediated signaling T3 Piersma et al 2013 Van der Burg et al 2013 RAR CALUX Retinoic acid receptor mediated signaling retinoic acid Piersma et al 2013 Van der Burg et al 2013 PPARγ CALUX PPARγ mediated signaling rosiglitazone Gijsbers et al 2011 PPARα CALUX PPARα mediated signaling GW7674 Gijsbers submitted PPARδ CALUX PPARδ mediated signaling L165041 Szalowska et al 2014 LXR CALUX LXR mediated signaling GW3965 unpublished kappaB CALUX NFκB mediated signaling TPA Piersma et al 2013 Van der Burg et al 2013 P21 CALUX Transcription of p21 inhibitor of cell cycle progression actinomycin D Piersma et al 2013 Van der Burg et al 2013 Nrf2 CALUX Activation of the Nrf2 pathway oxidative stress

    Original URL path: http://www.biodetectionsystems.com/products/bioassays/available-assays.html (2016-02-10)
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  • Upcoming events - Biodetection Systems
    up products Applications Food Feed Product Safety Human health epidemiology Chemicals pharmaceuticals Complex mixtures Environment Water quality Innovations Expertise Facilities Projects Contract research News Downloads News Events Publications Downloads Contact Contact information Directions International partners Login Print News Events Upcoming

    Original URL path: http://www.biodetectionsystems.com/news-downloads/events/upcoming-events.html (2016-02-10)
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  • Past events - Biodetection Systems
    Applications Food Feed Product Safety Human health epidemiology Chemicals pharmaceuticals Complex mixtures Environment Water quality Innovations Expertise Facilities Projects Contract research News Downloads News Events Publications Downloads Contact Contact information Directions International partners Login Print News Events Upcoming events Past events Publications Downloads Past events BioDetectors Conference 2014 BioDetectors Conference 2013 BioDetectors Conference 2012 DEMEAU workshop On 29th January 2015 our experts hosted and attended the DEMEAU workshop in Paris

    Original URL path: http://www.biodetectionsystems.com/news-downloads/events/past-events.html (2016-02-10)
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