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  • Cell Research
    Surgery McGill University Montreal Canada Our previous work showed that the cartilage proteoglycan aggrecan could induce an erosive polyarthritis and spondylitis in BALB c mice and the G1 globular domain of the aggrecan G1 contained the arthritogenic region To elucidate whether autoreactive T cells to G1 are expressed in rheumatoid arthritis patients we analyzed the frequency of human G1 specific T cells in the peripheral blood of five rheumatoid arthritis patients and tried to establish G1 reactive T cell lines from these rheumatoid arthritis patients The results showed that the G1 specific T cells in PBL were detectable at the range of 4 97 0 5 10 6 in peripheral blood lymphocytes We have also generated 15 G1 specific T lymphocyte lines from these pateints with a standard split well method All these cells expressed fine specificity to human recombinant G1 but not to unrelated antigen All the 15 lines expressed a pan T cell marker and 13 of them selectively used the ab T cell receptor Two of them used gd T cell receptor The 13 of these T cell lines was CD4 positive One line expressed CD8 One line expressed both CD4 and CD8 Moreover 14 out of

    Original URL path: http://www.cell-research.com/arts.asp?id=1447 (2016-02-14)
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  • Cell Research
    the testicular morphology MA Ying Hua 1 Jia Hua HU 1 Xiao Gang ZHOU 1 Zhen Tong MEI 2 Jian FEI 1 Li He GUO 1 1 Shanghai Institute of Cell Biology Chinese Academy of Science Shanghai 200031 China 2 Shanghai Institute of Physiology Chinese Academy of Science Shanghai 200031 China g Aminobutyric acid and GABAergic receptors were previously reported to be distributed in reproductive systems besides CNS and predicted to participate in the modulation of testicular function g Aminobutyric acid transporter was implicated to be involved in this process However the potential role of g aminobutyric transporter in testis has not been explored In this study we investigated the existence of mouse g aminobutyric acid transporter subtype I m GAT1 in testis Wild type and transgenic mice which overexpressing m GAT1 in a variety of tissues especially in testis were primarily studied to approach the profile of m GAT1 in testis Mice with overexpressed m GAT1 develop normally but with reduced mass and size of testis as compared with wild type Testicular morphology of transgenic mice exhibited overt abnormalities including focal damage of the spermatogenic epithelium accompanied by capillaries proliferation and increased diameter of seminiferous tubules lumen Reduced number

    Original URL path: http://www.cell-research.com/arts.asp?id=1449 (2016-02-14)
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  • Cell Research
    in human hepatoma cells ZHANG Ru Gang Xing Wang WANG Jin Hui YUAN Li Xia GUO Hong XIE Shanghai Institute of Cell Biology Chinese Academy of Sciences Shanghai 200031 China A non radioisotopic quantitative TRAP based telomerase activity assay was established mainly by using SYBR Green I staining instead of radioisotope Comparing with conventional radioisotope based method it was better in reproducibility and accuracy Using this method we found telomerase

    Original URL path: http://www.cell-research.com/arts.asp?id=1450 (2016-02-14)
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  • Cell Research

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    Original URL path: /artsmore1.asp?id=127 (2016-02-14)


  • Cell Research
    Issue Top 10 VOLUME 10 ISSUE 2 6 2000 81 92 Leptin a multifunctional hormone HUANG Lu 1 Cai LI 1 2 1 Touchstone Center for Diabetes Research 1 Departments of Physiology and 2 Departments of Internal Medicine The University of Texas Southwestern Medical Center 5323 H Hines Boulevard Dallas TX 75390 8854 USA Leptin is the protein product encoded by the obese ob gene It is a circulating hormone produced primarily by the adipose tissue ob ob mice with mutations of the gene encoding leptin become morbidly obese infertile hyperphagic hypothermic and diabetic Since the cloning of leptin in 1994 our knowledge in body weight regulation and the role played by leptin has increased substantially We now know that leptin signals through its receptor OB R which is a member of the cytokine receptor superfamily Leptin serves as an adiposity signal to inform the brain the adipose tissue mass in a negative feedback loop regulating food intake and energy expenditure Leptin also plays important roles in angiogenesis immune function fertility and bone formation Humans with mutations in the gene encoding leptin are also morbidly obese and respond to leptin treatment demonstrating that enhancing or inhibiting leptin s activities in

    Original URL path: http://www.cell-research.com/arts.asp?id=1437 (2016-02-14)
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  • Cell Research
    Tie Lin WANG 1 Gang PEI 2 Zhi Qiang CHI 1 1 Shanghai Institute of Materia Medica 2 Shanghai Institute of Cell Biology Shanghai Academy of Life Sciences Chinese Academy of Sciences Shanghai 200031 China Human m opioid receptor H m OR with a tag of six consecutive histidines at its carboxyl terminus had been expressed in recombinant baculovirus infected Sf9 insect cells The maximal binding capacity for the 3 H diprenorphine and 3 H ohmefentanyl Ohm were 9 1 0 7 and 6 52 0 23 nmol g protein respectively The 3 H diprenorphine or 3 H Ohm binding to the receptor expressed in Sf9 cells was strongly inhibited by m selective agonists D Ala 2 N methyl Phe 4 glyol 5 enkephalin DAGO Ohm and morphine but neither by d nor by k selective agonist Na 100 m M and GTP 50 m M could reduce H m OR agonists etorphine and Ohm affinity binding to the overexpressed H m OR m selective agonists DAGO and Ohm effectively stimulated 35 S GTP g S binding EC 50 2 7 n M and 6 9 n M and inhibited forskolin stimulated cAMP accumulation IC 50 0 9 n M

    Original URL path: http://www.cell-research.com/arts.asp?id=1438 (2016-02-14)
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  • Cell Research
    Sciences Beijing China 3 Visiting scientist from Beijing Agricultural University Beijing China 4 Department of Horticulture and Center for Gene Research and Biotechnology Oregon State University Corvallis OR 97331 USA Plasma membrane PM Ca 2 ATPase activity in poplar apical bud meristematic cells during short day SD induced dormancy development was examined by a cerium precipitation EM cytochemical method Ca 2 ATPase activity indicated by the status of cerium phosphate precipitated grains was localized mainly on the interior face cytoplasmic side of the PM when plants were grown under long days and reached a deep dormancy A few reaction products were also observed on the nuclear envelope When plant buds were developing dormancy after 28 to 42 d of SD exposure almost no reaction products were present on the interior face of the PM In contrast a large number of cerium phosphate precipitated grains were distributed on the exterior face of the PM After 70 d of SD exposure when buds had developed a deep dormancy the reaction products of Ca 2 ATPase activity again appeared on the interior face of the PM The results seemed suggesting that two kinds of Ca 2 ATPases may be present on the PM

    Original URL path: http://www.cell-research.com/arts.asp?id=1439 (2016-02-14)
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  • Cell Research
    of Cell Biology Ministry of Public Health of China Institute of Medical Molecular Biology China Medical University 92 Beier Rd Heping District Shenyang 110001 China The expression of glucose regulated protein 94 GRP94 during the treatment of human colorectal carcinoma cell line Clone A cells with sodium butyrate was studied Sodium butyrate SB can cause functional and morphological effects on Clone A cells including growth arrest at G 0 G

    Original URL path: http://www.cell-research.com/arts.asp?id=1440 (2016-02-14)
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