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  • Cell Research
    WANG 2 Shi Jin XIU 2 Zheng Wen JIANG 2 Shuang YANG 2 Xin Tai ZHAO 1 Wei HUANG 2 Jian Ren GU 1 1 National Laboratory for Oncogene and Related Genes Shanghai Cancer Institute 25 2200 Xie tu Rd Shanghai 200032 China 2 Chinese Human Genome Center at Shanghai 351 Guo Shou Jin Road Zhangjiang High Tech Park Shanghai 201203 China To elucidate the molecular pathology underlying the development of hepatocellular carcinoma HCC we used 41 highly polymorphic microsatellite markers to examine 55 HCC and corresponding non tumor liver tissues on chromosome 9 16 and 17 Loss of heterozygosity LOH is observed with high frequency on chromosomal region 17p13 36 55 65 9p21 p23 28 55 51 16q21 q23 27 55 49 in tumors Meanwhile microsatellite instability is rarely found in these microsatellite loci Direct sequencing was performed to detect the tentative mutation of tumor suppressor genes in these regions p53 MTS1 p16 and CDH1 E cadherin Within exon 5 9 of p53 gene 14 out of 55 HCC specimens 24 have somatic mutations and nucleotide deletion of this gene is reported in HCC for the first time Mutation in MTS1 p16 is found only in one tumor case

    Original URL path: http://www.cell-research.com/arts.asp?id=1427 (2016-02-14)
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  • Cell Research
    Wei ZHANG 1 Feng Ling LI 1 Ying Li GUO 2 Tian Lei LIU 1 Hai HUANG 1 3 1 Shanghai Institute of Plant Physiology Chinese Academy of Science 300 Fenglin Road Shanghai 200032 China 2 School of Life Sciences University of Science and Technology of China P O Box 4 Hefei Anhui 230027 China 3 Shanghai Research Center of Life Sciences Chinese Academy of Sciences 320 Yueyang Road Shanghai 200031 China Molecular and genetic characterizations of mutants have led to a better understanding of many developmental processes in the model system Arabidopsis thaliana However the leaf development that is specific to plants has been little studied With the aim of contributing to the genetic dissection of leaf development we have performed a large scare screening for mutants with abnormal leaves Among a great number of leaf mutants we have generated by T DNA and transposon tagging and ethylmethae sulfonate EMS mutagenesis four independent mutant lines have been identified and studied genetically Phenotypes of these mutant lines represent the defects of four novel nuclear genes designated LL1 LOTUS LEAF 1 LL2 LOTUS LEAF 2 URO UPRIGHT ROSETTE and EIL ENVIRONMENT CONDITION INDUCED LESION The phenotypic analysis indicates that these genes

    Original URL path: http://www.cell-research.com/arts.asp?id=1428 (2016-02-14)
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  • Cell Research

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    Original URL path: /artsmore1.asp?id=124 (2016-02-14)


  • Cell Research
    Fan Xiao Qiang Ya Jun Guo International Joint Cancer Institute The Second Military Medical University Shanghai 200433 China Apoptosis is a complex process involving a large array of genes and mutation of any of these genes may lead to malignancy formation Re acquirement of FasL by tumor cells may enable them to evade the surveillance of immune system and thus contributes to the growth of tumor Apart from traditional therapies

    Original URL path: http://www.cell-research.com/arts.asp?id=1411 (2016-02-14)
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  • Cell Research
    in the regulation of HIV LTR by thyroid hormone receptor Shao Chung Victor HSIA Hua WANG Yun Bo SHI Unit on Molecular Morphogenesis Laboratory of Molecular Embryology National Institute of Child Health and Human Development National Institute of Health Building 18T Room 106 Bethesda MD 20892 5431 USA The HIV 1 LTR controls the expression of HIV 1 viral genes and thus is critical for viral propagation and pathology Numerous host factors have been shown to participate in the regulation of the LTR promoter Among them is the thyroid hormone T 3 receptor TR TR has been shown to bind to the critical region of the promoter that contain the NFβB and Sp1 binding sites Interestingly earlier transient transfection studies in tissue culture cells have yielded contradicting conclusions on the role of TR in LTR regulation likely due to the use of different cell types and or lack of proper chromatin organization Here using the frog oocyte as a model system that allows replication coupled chromatin assembly mimicking that in somatic cells we demonstrate that unliganded heterodimers of TR and RXR 9 cis retinoic acid receptor repress LTR while the addition of T 3 relieves the repression and further activates

    Original URL path: http://www.cell-research.com/arts.asp?id=1412 (2016-02-14)
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  • Cell Research
    Fang GUI State Key laboratory of Freshwater Ecology and Biotechnology Institute of Hydrobiology Chinese Academy of Sciences Wuhan 430072 China Gynogenetic silver crucian carp Carassius auratus gibelio is an intriguing model system In the present work a systemic study has been initiated by introducing suppression subtractive hybridization technique into this model system to identify the differentially expressed genes in oocytes between gynogenetic silver crucian carp and its closely related gonochoristic color crucian carp Five differential cDNA fragments were identified from the preliminary screening and two of them are ZP3 homologues Moreover the full length ZP3 cDNAs were cloned from their oocyte cDNA libraries The length of ZP3 cDNAs were 1378 bp for gyno carp and 1367 bp for gono carp and they can be translated into proteins with 435 amino acids Obvious differences are not only in the composition of amino acids but also in the number of potential O linked oligosaccharide sites In addition gyno carp ZP3 amino acid sequence has an unexpected higher identity value with common carp 83 5 than that with the closely related gono carp 74 7 The unique homology may be originated from the ancient hybridization Northern blot analysis confirmed that expression of the

    Original URL path: http://www.cell-research.com/arts.asp?id=1413 (2016-02-14)
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  • Cell Research
    11 ISSUE 1 3 2001 28 36 Partial characterization of the N linked oligosaccharide structures on P selectin glycoprotein ligand 1 PSGL 1 AEED Paul A 1 Jian Guo GENG 2 Darwin ASA 1 Loretta RAYCROFT 1 Li MA 1 Åke P ELHAMMER 1 1 Pharmacia Corp 7000 Portage Road Kalamazoo MI 49001 0199 USA 2 Institute of Biochemistry and Cell Biology Shanghai Institute for Biological Sciences Chinese Academy of Sciences 320 Yueyang Road Shanghai 200031 China PSGL 1 a specific ligand for P E and L selectin was isolated from in vivo 3 H glucosamine labeled HL 60 cells by a combination of wheat germ agglutinin agarose and P or E selectin agarose chromatography N linked oligosaccharides were released from the purified denatured ligand molecule by peptide N glycosidase F treatment and following separation by Sephacryl S 200 chromatography partially characterized using lectin ion exchange and size exclusion chromatography in combination with glycosidase digestions The data obtained suggest that the N glycans on PSGL 1 are predominantly core fucosylated multiantennary complex type structures with extended poly N acetyllactosamine containing outer chains A portion of the outer chains appears to be substituted with fucose indicating that the N glycans in

    Original URL path: http://www.cell-research.com/arts.asp?id=1414 (2016-02-14)
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  • Cell Research
    43 Hydrogen peroxide induced changes in intracellular pH of guard cells precede stomatal closure ZHANG Xiao 1 2 Fa Cai DONG 1 Jun Feng GAO 2 Chun Peng SONG 1 1 Department of Biology Henan University Kaifeng 475001 China 2 College of Life Sciences Northwest Sci Tech University of Agriculture and Forestry Yangling 712100 China Epidermal bioassay demonstrated that benzylamine a membrane permeable weak base can mimick hydrogen peroxide H 2 O 2 to induce stomatal closure and butyric acid a membrane permeable weak acid can partly abolish the H 2 O 2 induced stomatal closure Confocal pH mapping with the probe 5 and 6 carboxy seminaphthorhodafluor 1 acetoxymethylester SNARF 1 AM revealed that H 2 O 2 leads to rapid changes in cytoplasmic and vacuolar pH in guard cells of Vicia faba L i e alkalinization of cytoplasmic areas occur red in parallel with a decrease of the vacuolar pH and that butyric acid pretreatment can abolish alkalinization of cytoplasmic areas and acidification of vacuolar areas of guard cells challenged with H 2 O 2 These results imply that the alkalinization of cytoplasm via efflux of cytosol protons into the vacuole in guard cells challenged with H 2 O

    Original URL path: http://www.cell-research.com/arts.asp?id=1415 (2016-02-14)
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