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  • Cell Research
    B lymphocytes alters the IL 4 activated IRS2 signaling pathway in a STAT6 independent manner implications for cell survival and proliferation ZAMORANO Jose Ann E KELLY Jonathan AUSTRIAN Helen Y WANG Achsah D KEEGAN Department of Immunology Jerome Holland Labs American Red Cross Rockville MD USA IL 4 is an important B cell survival and growth factor IL 4 induced the tyrosine phosphorylation of IRS2 in resting B lymphocytes and in LPS or CD40L activated blasts Phosphorylated IRS2 coprecipitated with the p85 subunit of PI 3 kinase in both resting and activated cells By contrast association of phosphorylated IRS2 with GRB2 was not detected in resting B cells after IL 4 treatment although both proteins were expressed However IL 4 induced association of IRS2 with GRB2 in B cell blasts The pattern of IL 4 induced recruitment of p85 and GRB2 to IRS2 observed in B cells derived from STAT6 null mice was identical to that observed for normal mice While IL 4 alone does not induce activation of MEK a MEK1 inhibitor suppressed the IL 4 induced proliferative response of LPS activated B cell blasts These results demonstrate that costimulation of splenic B cells alters IL 4 induced signal

    Original URL path: http://www.cell-research.com/arts.asp?id=1416 (2016-02-14)
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  • Cell Research
    11 ISSUE 1 3 2001 55 60 p53 independent upregulation of p21 WAF1 in NIH 3T3 cells malignantly transformed by mot 2 TAKANO Syuichi 1 2 Renu WADHWA 3 Youji MITSUI 1 2 Sunil C KAUL 1 1 National Institute of Bioscience and Human Technology AIST 1 1 Higashi Tsukuba Ibaraki 305 8566 Japan 2 Institute of Applied Biochemistry University of Tsukuba Tsukuba Ibaraki 305 8577 Japan 3 Chugai Research Institute for Molecular Medicine 153 2 Nagai Niihari mura Ibaraki 300 41 Japan Mot 2 protein is shown to interact with p53 and inhibit its transcriptional activation function Mot 2 overexpressing stable clones of NIH 3T3 cells were malignantly transformed however they had a high level of expression of a p53 downstream gene p21 WAF1 The present study was undertaken to elucidate possible molecular mechanism s of such upregulation An increased level of p21 WAF1 expression was detected in stable transfectants although an exogenous reporter gene driven by p21 WAF1 promoter exhibited lower activity in these cells suggesting that some post transcriptional mechanism contributes to upregulation Western analyses of transient and stable clones revealed that upregulation of p21 WAF1 in stable NIH 3T3 mot 2 cells may be mediated by

    Original URL path: http://www.cell-research.com/arts.asp?id=1417 (2016-02-14)
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  • Cell Research
    Issue Top 10 VOLUME 11 ISSUE 1 3 2001 61 67 Overexpression of γ aminobutyric acid transporter subtype I leads to susceptibility to kainic acid induced seizure in transgenic mice MA Ying Hua 1 Jia Hua HU 1 Wen Juan ZHAO 1 Jian FEI 1 Yun YU 1 Xiao Gang ZHOU 1 Zhen Tong MEI 2 Li He GUO 1 1 Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai 200031 China 2 Shanghai Institute of Physiology Chinese Academy of Sciences Shanghai 200031 China γ aminobutyric acid GABA is the principal inhibitory neurotransmitter and the GABAergic synaptic transmission is normally terminated by the rapid uptake through GABA transporters With transgenic mice ubiquitously overexpressing GABA transporter subtype I GAT1 the present study explored the pathophysiological role of GAT1 in epileptogenesis Though displaying no spontaneous seizure activity these mice exhibit altered electroencephalographic patterns and increased susceptibility to seizure induced by kainic acid In addition the GABA A receptor and glutamate transporters are up regulated in transgenic mice which perhaps reflects a compensatory or corrective change to the elevated level of GAT1 These preliminary findings support the hypothesis that excitatory and inhibitory neurotransmission and seizure susceptibility

    Original URL path: http://www.cell-research.com/arts.asp?id=1418 (2016-02-14)
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  • Cell Research
    Publication Current Issue Top 10 VOLUME 11 ISSUE 1 3 2001 68 73 Structural components of the nuclear body in nuclei of Allium cepa cells TAO Wei 1 Chang Hui YAN 1 Tao CAI 1 Shui HAO 2 Zhong He ZHAI 1 1 College of Life Sciences Peking University Beijing 100871 China 2 Institute of Genetics and Cytology Northeast Normal University Changchun Jilin 130024 China Nuclear bodies have long been noted in interphase nuclei of plant cells but their structural component origin and function are still unclear by now The present work showed in onion cells the nuclear bodies appeared as a spherical structure about 0 3 to 0 8 μm in diameter They possibly were formed in nucleolus and subsequently released and entered into nucleoplasm Observation through cytochemical staining method at the ultrastructural level confirmed that nuclear bodies consisted of ribonucleoproteins RNPs and silver stainable proteins Immunocytochemical results revealed that nuclear bodies contained no DNA and ribosomal gene transcription factor UBF Based on these data we suggested that nuclear bodies are not related to the ribosome or other gene transcription activities instead they may act as subnuclear structures for RNPs transport from nucleolus to cytoplasm and may also be

    Original URL path: http://www.cell-research.com/arts.asp?id=1419 (2016-02-14)
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  • Cell Research
    1 Guo Yong AN 2 Zhi Ping ZHU 1 1 Shanghai Institute of Plant Physiology Chinese Academy of Sciences Shanghai 200032 China 2 Department of Biology Henan University Kaifeng 475001 China Bicoid is one of the important Drosophila maternal genes involved in the control of embryo polarity and larvae segmentation To clone and characterize the rice bicoid related genes one cDNA clone Rb24 EMBL accession number AJ2771380 was isolated by screening of rice unmature seed cDNA library Sequence analysis indicates that Rb24 contains a putative amino acid sequence which is homologous to unique 8 amino acids sequence within Drosophila bicoid homeodomain 50 identity 75 similarity and involves a lys 9 in putative helix 3 Northern blot analysis of rice RNA has shown that this sequence is expressed in a tissue specific manner The transcript was detected strongly in young panicles but less in young leaves and roots This results are further confirmed with paraffin section in situ hybridization The signal is intensive in rice globular embryo and located at the apical tip of the embryo then along with the development of embryo the signal is getting reduced and transfers into both sides of embryo The existence of bicoid related sequence

    Original URL path: http://www.cell-research.com/arts.asp?id=1420 (2016-02-14)
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  • Cell Research
    Current Issue Top 10 VOLUME 11 ISSUE 1 3 2001 81 84 Melanocortin 1 receptor gene variants in four Chinese ethnic populations SHI Peng Xue Mei LU Huai Rong LUO Jin Gong XIANG YU Ya Ping ZHANG Laboratory of Cellular and Molecular Evolution Kunming Institute of Zoology the Chinese Academy of Sciences Kunming 650223 China There is strong relationship between melanocortin 1 receptor MC1R gene variants and human hair color and skin type Based on a sequencing study of MC1R gene in 50 individuals from the Uygur Tibetan Wa and Dai ethnic populations we discuss the occurrence of 7 mc1r variants consisting of 5 nonsynonymous sites Val60Leu Arg67Gln Val92Met Arg163Gln and Ala299Val and 2 synonymous sites C414T and A942G among which C414T and Ala299Val were reported for the first time Confirmation and analysis were also made of 122 individuals at three common point mutations Val92Met Arg163Gln A942G using PCR SSCP The frequency of Arg163Gln variant varies in the four ethnic populations with percentage of 40 85 0 66 2 and 72 7 respectively while those of Val92Met and A942G are roughly similar in these four populations The different environments migration and admixture of various ethnic groups in China might have

    Original URL path: http://www.cell-research.com/arts.asp?id=1421 (2016-02-14)
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  • Cell Research

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    Original URL path: /artsmore1.asp?id=123 (2016-02-14)


  • Cell Research
    Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences 320 Yue Yang Road Shanghai 200031 China Directional migration of leukocytes is indispensable to innate immunity for host defense However recruitment of leukocytes to a site of tissue injury also constitutes a leading cause for inflammatory responses Mechanistically it involves a cascade of cellular events precisely regulated by temporal and spatial presentation of a repertoire

    Original URL path: http://www.cell-research.com/arts.asp?id=1399 (2016-02-14)
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