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  • Cell Research
    10 VOLUME 11 ISSUE 3 9 2001 217 222 TNF receptor associated factor 2 binding site is involved in TNFR75 dependent enhancement of TNFR55 induced cell death LU Fang Jia FANG Chang Qing CHEN Shanghai Research Center of Biotechnology Shanghai Institutes for Biological Sciences the Chinese Academy of Sciences Shanghai 200233 China TNF recepter 55 is the main mediator of TNF induced apoptosis TNF receptor 75 dependent induction or enhancement of cytotoxicity has been explained by intracellular signaling ligand passing or induction of endogenous TNF To study the function of human TNF receptor 75 hTR75 and the interaction between human TNF receptor 55 hTR55 and hTR75 in hTNF a induced cytotoxicity HEp 2 cells were transfected with bicistronic expression vector of h TR 75 and its deletion mutants genes hTNF a induced cytotoxicity was determined by crystal violet colorimetric method The expression of hTR75 and its deletion mutants in HEp 2 cells was demonstrated by RT PCR and indirect ELISA We found that the overexpressed hTR75 could significantly increase the susceptibility of HEp 2 cells to hTNF a which especi a lly required TRAF2 binding site hTR75 could not only mediate partial hTNF a induced cytotoxicity independently but also fulfill

    Original URL path: http://www.cell-research.com/arts.asp?id=1395 (2016-02-14)
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  • Cell Research
    homologue of polarity protein Par 3 of Caenorhabditis elegans and Bazooka of Drosophila melanogaster Asip Par 3 Bazooka are PDZ motif containing proteins that localize asymmetrically to the cell periphery and play a pivotal role in cell polarity and asymmetric cell division In the present study we have cloned human asip cDNA and its splicing variants by 5 RACE and RT PCR using candidate human EST clones which have a high homology to rat asip cDNA The full length cDNA of human asip encodes a 1 353 aa protein exhibiting 88 similarity to the rat one Human asip is a single copy gene consisting of at least 26 exons and localizing in human chromosome 10 band p11 2 with some extraordinarily long introns All exon intron boundary nucleotides conform to the gt ag rule Three main transcripts were detected by Northern blot analysis and at least five variants from alternative splicing and polyadenylation have been identified by RT PCR and liver cDNA library screening Exon 17b deleted asip mRNAs expressed ubiquitously in normal human tissues including liver on RT PCR analysis However they were absent from most human liver cancer cell lines examined More interestingly the expression of exon 17b

    Original URL path: http://www.cell-research.com/arts.asp?id=1396 (2016-02-14)
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  • Cell Research
    of Biological Sciences University of South Carolina 700 Sumter Street Columbia SC29208 USA 2 National Laboratory of Plant Molecular Genetics Shanghai Institute of Plant Physiology and Ecology Chinese Academy of Sciences 300 Fenglin Road Shanghai 200032 China Communicated by László Márton Department of Biological Sciences University of South Carolina 700 Sumter Street Columbia SC29208 USA Mercury pollution is a major environmental problem accompanying industrial activities Most of the mercury released ends up and retained in the soil as complexes of the toxic ionic mercury Hg 2 which then can be converted by microbes into the even more toxic methylmercury which tends to bioaccumulate Mercury detoxification of the soil can also occur by microbes converting the ionic mercury into the least toxic metallic mercury Hg 0 form which then evaporates The remediation potential of transgenic plants carrying the MerA gene from E coli encoding mercuric ion reductase could be evaluated A modified version of the gene optimized for plant codon preferences merApe9 Rugh et al 1996 was introduced into tobacco by Agrobacterium mediated leaf disk transformation Transgenic seeds were resistant to HgCl 2 at 50 m M and some of them 10 20 could germinate on media containing as much as 350 m M HgCl 2 while the control plants were fully inhibited or died on 50 m M HgCl 2 The rate of elemental mercury evolution from Hg 2 added as HgCl 2 was 5 8 times higher for transgenic plants than the control Mercury volatilization by isolated organs standardized for fresh weight was higher up to 5 times in the roots than in shoots or the leaves The data suggest that it is the root system of the transgenic plants that volatilizes most of the reduced mercury Hg 0 It also suggests that much of the mercury need not

    Original URL path: http://www.cell-research.com/arts.asp?id=1397 (2016-02-14)
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  • Cell Research
    of foreign chimeric genes in loblolly pine Pinus taeda L Transformation was achieved by co cultivation of mature zygotic embryos with Agrobacterium tumefaciens strain LBA4404 which harbored a binary vector pBI121 including genes for b glucuronidase GUS and neomycin phosphotransferase NPTII Factors influencing transgene expression including seed sources of loblolly pine concentration of bacteria and the wounding procedures of target explants were investigated The expression of foreign gene was confirmed

    Original URL path: http://www.cell-research.com/arts.asp?id=1398 (2016-02-14)
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  • Cell Research

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    Original URL path: /artsmore1.asp?id=121 (2016-02-14)


  • Cell Research
    ISSUE 4 12 2001 245 252 Thyroid hormone regulation of apoptotic tissue remodeling during anuran metamorphosis SHI Yun Bo Liezhen FU Shao Chung Victor HSIA Akihiro TOMITA Daniel BUCHHOLZ Laboratory of Gene Regulation and Development National Institute of Child Health and Human Development National Institutes of Health Bethesda Maryland 20892 5431 USA Anuran metamorphosis involves systematic transformations of individual organs in a thyroid hormone TH dependent manner Morphological and cellular studies have shown that the removal of larval organs tissues such the tail and the tadpole intestinal epithelium is through programmed cell death or apoptosis Recent molecular investigations suggest that TH regulates metamorphosis by regulating target gene expression through thyroid hormone receptors TRs which are DNA binding transcription factors Cloning and characterization of TH response genes show that diverse groups of early response genes are induced by TH The products of these TH response genes are believed to directly or indirectly affect the expression and or functions of cell death genes which are conserved at both sequence and function levels in different animal species A major challenge for future research lies at determining the signaling pathways leading to the activation of apoptotic processes and whether different death genes are involved

    Original URL path: http://www.cell-research.com/arts.asp?id=1378 (2016-02-14)
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  • Cell Research
    Online Publication Current Issue Top 10 VOLUME 11 ISSUE 4 12 2001 253 264 Molecular signal transduction in vascular cell apoptosis GENG Yong Jian Center for Cardiovascular Biology and Atherosclerosis Research Division of Cardiology Department of Internal Medicine University of Texas Houston Health Science Center Medical School 6431 Fannin Street MSB 6 045 Houston TX 77030 USA Apoptosis is a form of genetically programmed cell death which plays a key role in regulation of cellularity in a variety of tissue and cell types including the cardiovascular tissues Under both physiological and pathophysiological conditions various biophysiological and biochemical factors including mechanical forces reactive oxygen and nitrogen species cytokines growth factors oxidized lipoproteins etc may influence apoptosis of vascular cells The Fas Fas ligand caspase death signaling pathway Bcl 2 protein family mitochondria the tumor suppressive gene p53 and the proto oncogene c myc may be activated in atherosclerotic lesions and mediates vascular apoptosis during the development of atherosclerosis Abnormal expression and dysfunction of these apoptosis regulating genes may attenuate or accelerate vascular cell apoptosis and affect the integrity and stability of atherosclerotic plaques Clarification of the molecular mechanism that regulates apoptosis may help design a new strategy for treatment of atherosclerosis

    Original URL path: http://www.cell-research.com/arts.asp?id=1379 (2016-02-14)
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  • Cell Research
    Shi Qian 1 Wei Dong YONG 1 Wen Zhong XU 1 Yun Yuan XU 1 Jin Song WU 1 Kang CHONG 1 Ke Hui TAN 1 Zhi Hong XU 1 2 1 Research Center for Molecular and Developmental Biology Institute of Botany Chinese Academy of Sciences Beijing 100093 China 2 Chinese Academy of Sciences Beijing 100864 China The presence of DNA homologues corresponding to verc203 vernalization related cDNA clone was investigated by molecular hybridization techniques The genes were detected in 16 plant species that cover 12 subclasses of the Takhtajan system of angiosperms classification including diverse model species The results of Southern blot analysis showed a low copy number of this gene existed in rice wheat barley and Arabidopsis The hybridization result of PCR products demonstrated the conservation of the gene corresponding to ver203 in diverse plants The phylogenetic tree of the ver203 gene in tested plants was supported by evolution relationship of species The ver203 gene expressed in a vernalized plumule winter wheat instead of the root And the endosperm before the treatment was essential for the ver203 expression during vernalization in wheat In Arabidopsis thaliana the pattern of expression showed that the gene corresponding to ver203 was expressed

    Original URL path: http://www.cell-research.com/arts.asp?id=1380 (2016-02-14)
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