archive-com.com » COM » C » CELL-RESEARCH.COM

Total: 1754

Choose link from "Titles, links and description words view":

Or switch to "Titles and links view".
  • Cell Research
    gmail com Jiahuai Han E mail jhan xmu edu cn Inflammasome is an intracellular signaling complex of the innate immune system Activation of inflammasomes promotes the secretion of interleukin 1β IL 1β and IL 18 and triggers pyroptosis Caspase 1 and 11 or 4 5 in human in the canonical and non canonical inflammasome pathways respectively are crucial for inflammasome mediated inflammatory responses Here we report that gasdermin D GSDMD is another crucial component of inflammasomes We discovered the presence of GSDMD protein in nigericin induced NLRP3 inflammasomes by a quantitative mass spectrometry based analysis Gene deletion of GSDMD demonstrated that GSDMD is required for pyroptosis and for the secretion but not proteolytic maturation of IL 1β in both canonical and non canonical inflammasome responses It was known that GSDMD is a substrate of caspase 1 and we showed its cleavage at the predicted site during inflammasome activation and that this cleavage was required for pyroptosis and IL 1β secretion Expression of the N terminal proteolytic fragment of GSDMD can trigger cell death and N terminal modification such as tagging with Flag sequence disrupted the function of GSDMD We also found that pro caspase 1 is capable of processing GSDMD

    Original URL path: http://www.cell-research.com/arts.asp?id=2192 (2016-02-14)
    Open archived version from archive


  • Cell Research
    modulating amino acid signaling to mTORC1 FREE Carsten Gram Hansen 1 Yuen Lam Dora Ng 1 Wai Ling Macrina Lam 1 Steven W Plouffe 1 and Kun Liang Guan 1 1 Department of Pharmacology and Moores Cancer Center University of California San Diego La Jolla CA 92093 USA Correspondence Kun Liang Guan Tel 1 8588227945 Tel Kun Liang Guan Tel 1 8588227945 E mail kuguan ucsd edu YAP and TAZ are transcriptional co activators and function as the major effectors of the Hippo tumor suppressor pathway which controls cell growth tissue homeostasis and organ size Here we show that YAP TAZ play an essential role in amino acid induced mTORC1 activation particularly under nutrient limiting conditions Mechanistically YAP TAZ act via the TEAD transcription factors to induce expression of the high affinity leucine transporter LAT1 which is a heterodimeric complex of SLC7A5 and SLC3A2 Deletion of YAP TAZ abolishes expression of LAT1 and reduces leucine uptake Re expression of SLC7A5 in YAP TAZ knockout cells restores leucine uptake and mTORC1 activation Moreover SLC7A5 knockout cells phenocopies YAP TAZ knockout cells which exhibit defective mTORC1 activation in response to amino acids We further demonstrate that YAP TAZ act through SLC7A5 to

    Original URL path: http://www.cell-research.com/arts.asp?id=2193 (2016-02-14)
    Open archived version from archive

  • Cell Research
    Dong Li 5 Zhengmao Zhu 6 Enkui Duan 3 Erlie Jiang 1 2 Sizhou Feng 1 2 Mingzhe Han 1 2 Yuanfu Xu 1 2 Fei Wang 7 and Jiaxi Zhou 1 2 1 State Key Laboratory of Experimental Hematology Institute of Hematology and Blood Diseases Hospital Chinese Academy of Medical Sciences Peking Union Medical College 288 Nanjing Road Tianjin 300020 China 2 Center for Stem Cell Medicine Chinese Academy of Medical Sciences Department of Stem Cells and Regenerative Medicine Peking Union Medical College Beijing China 3 State Key Laboratory of Reproductive Biology Institute of Zoology CAS Beijing 100101 China 4 College of Life Sciences at Yangtze University Jingzhou Hubei 434025 China 5 Department of Oncology Shanghai Third People s Hospital School of Medicine Shanghai Jiaotong University Shanghai 201900 China 6 College of Life Sciences Nankai University Tianjin 300071 China 7 Department of Cell and Developmental Biology and Institute for Genomic Biology University of Illinois at Urbana Champaign Urbana IL 61801 USA Correspondence Jiaxi Zhou Tel 86 22 23909412 Fax 86 22 23909412 E mail zhoujx ihcams ac cn How BMP signaling integrates into and destabilizes the pluripotency circuitry of human pluripotent stem cells hPSCs to initiate differentiation into individual germ layers is a long standing puzzle Here we report muscle segment homeobox 2 MSX2 a homeobox transcription factor of msh family as a direct target gene of BMP signaling and a master mediator of hPSCs differentiation to mesendoderm Enforced expression of MSX2 suffices to abolish pluripotency and induce directed mesendoderm differentiation of hPSCs while MSX2 depletion impairs mesendoderm induction MSX2 is a direct target gene of the BMP pathway in hPSCs and can be synergistically activated by Wnt signals via LEF1 during mesendoderm induction Furthermore MSX2 destabilizes the pluripotency circuitry through direct binding to the SOX2 promoter and repression

    Original URL path: http://www.cell-research.com/arts.asp?id=2188 (2016-02-14)
    Open archived version from archive

  • Cell Research
    Verreck 7 Maria del Carmen Sasiain 3 Olivier Neyrolles 1 2 Isabelle Maridonneau Parini 1 2 Geanncarlo Lugo Villarino 1 2 and Céline Cougoule 1 2 1 CNRS Institut de Pharmacologie et de Biologie Structurale IPBS Département of Tuberculosis and Infection Biology Toulouse France 2 Université de Toulouse Université Paul Sabatier UPS IPBS Toulouse France 3 Inmunologia de Enfermedades Respiratorias Instituto de Medicina Experimental IMEX CONICET Academia Nacional de Medicina Pacheco de Melo 3081 1425 Buenos Aires Argentina 4 INSERM UPS US006 CREFRE Service d Histopathologie CHU Purpan 31024 Toulouse France 5 Mycobacteria Unit Pasteur Institute in Antananarivo Antananarivo Madagascar 6 Instituto Prof Dr Raúl Vaccarezza Hospital de Infecciosas Dr F J Muñiz Buenos Aires Argentina 7 Department of Parasitology Biomedical Primate Research Centre Rijswijk the Netherlands Correspondence Isabelle Maridonneau Parini Tel 33 0 5 61 17 54 58 Fax 33 0 5 61 17 59 94 E mail Isabelle Maridonneau Parini ipbs fr Olivier Neyrolles E mail Olivier Neyrolles ipbs fr The human CD14 monocyte compartment is composed by two subsets based on CD16 expression We previously reported that this compartment is perturbed in tuberculosis TB patients as reflected by the expansion of CD16 monocytes along with disease severity Whether this unbalance is beneficial or detrimental to host defense remains to be elucidated Here in the context of active TB we demonstrate that human monocytes are predisposed to differentiate towards an anti inflammatory M2 like macrophage activation program characterized by the CD16 CD163 MerTK pSTAT3 phenotype and functional properties such as enhanced protease dependent motility pathogen permissivity and immunomodulation This process is dependent on STAT3 activation and loss of function experiments point towards a detrimental role in host defense against TB Importantly we provide a critical correlation between the abundance of the CD16 CD163 MerTK pSTAT3 cells and the

    Original URL path: http://www.cell-research.com/arts.asp?id=2189 (2016-02-14)
    Open archived version from archive

  • Cell Research
    Chua 5 Liang Yang 5 Deyu Zhu 2 Lichuan Gu 2 and Luyan Z Ma 1 1 State Key Laboratory of Microbial Resources Institute of Microbiology Chinese Academy of Sciences Beijing 100101 China 2 State Key Laboratory of Microbial Technology School of Life Sciences Shandong University Jinan Shandong 250100 China 3 University of the Chinese Academy of Sciences Beijing 100049 China 4 School of Food Science Washington State University Pullman WA 99164 6120 USA 5 Singapore Centre on Environmental Life Sciences Engineering SCELSE Nanyang Technological University Singapore 637551 Correspondence Luyan Z Ma E mail luyanma27 im ac cn Lichuan Gu E mail lcgu sdu edu cn Biofilms are surface associated communities of microorganism embedded in extracellular matrix Exopolysaccharide is a critical component in the extracellular matrix that maintains biofilm architecture and protects resident biofilm bacteria from antimicrobials and host immune attack However self produced factors that target the matrix exopolysaccharides are still poorly understood Here we show that PslG a protein involved in the synthesis of a key biofilm matrix exopolysaccharide Psl in Pseudomonas aeruginosa prevents biofilm formation and disassembles existing biofilms within minutes at nanomolar concentrations when supplied exogenously The crystal structure of PslG indicates the typical features of

    Original URL path: http://www.cell-research.com/arts.asp?id=2194 (2016-02-14)
    Open archived version from archive

  • Cell Research
    Bedzhov 1 Monika Bialecka 1 Agata Zielinska 1 Joanna Kosalka 1 Francesco Antonica 1 Amelia J Thompson 1 Kristian Franze 1 and Magdalena Zernicka Goetz 1 1 Department of Physiology Development and Neuroscience University of Cambridge Downing Street Cambridge CB2 3DY UK Correspondence Magdalena Zernicka Goetz E mail mz205 cam ac uk Establishment of the anterior posterior AP axis in the mouse embryo is crucial for proper induction of the germ layers Despite this importance it is unclear whether specification of the AP axis is an autonomous process or if it requires an embryonic maternal interaction at the time of implantation By embryonic day 5 5 E5 5 the combined activities of Nodal GDF3 and the Cripto co receptor generate a proximal distal gradient that establishes a signaling center at the distal tip of the embryo the distal visceral endoderm DVE This proximo distal asymmetry is transformed into the AP axis as the DVE cells migrate DVE cells express the Nodal antagonists Lefty1 and Cer1 which specify the anterior identity of the underlying epiblast1 The expression of these key DVE markers is already triggered before implantation2 3 4 Cells that express Lefty1 and Cer1 in the late blastocyst contribute to

    Original URL path: http://www.cell-research.com/arts.asp?id=2190 (2016-02-14)
    Open archived version from archive

  • Cell Research
    12 12 2015 1372 1375 CRISPR Cas9 mediated genome engineering of the ferret FREE Zhaohui Kou 1 Qian Wu 1 Xiaochen Kou 2 Chonghai Yin 1 Hong Wang 2 Zhentao Zuo 1 Yan Zhuo 1 Antony Chen 3 Shaorong Gao 2 and Xiaoqun Wang 1 1 State Key Laboratory of Brain and Cognitive Science CAS Center for Excellence in Brain Science and Intelligence Technology Shanghai Beijing MRI Centre for Brain Research Institute of Biophysics Chinese Academy of Sciences Beijing Institute for Brain Disorders Beijing 100101 China 2 Clinical and Translational Research Center of Shanghai First Maternity and Infant Health Hospital School of Life Sciences and Technology Tongji University Shanghai 200092 China 3 Wuxi Sangosho Biotechnology Corporations Wuxi Zhejiang 214142 China Correspondence Shaorong Gao E mail gaoshaorong tongji edu cn Xiaoqun Wang E mail xiaoqunwang ibp ac cn Ferrets Mustela putorius furo have served as one of the most valuable animal models for recapitulating human disease since 19331 This is because they show many similarities to humans in terms of physiological features of brain function and reproductive biology as well as pathological characteristics of various diseases such as cancer influenza infection and cystic fibrosis2 3 4 Despite these advantages the current

    Original URL path: http://www.cell-research.com/arts.asp?id=2191 (2016-02-14)
    Open archived version from archive

  • Cell Research
    Genome scale detection of hypermethylated CpG islands in circulating cell free DNA of hepatocellular carcinoma patients Lu Wen 1 Jingyi Li 1 Huahu Guo 2 4 5 Xiaomeng Liu 1 Shengmin Zheng 3 Dafang Zhang 3 Weihua Zhu 3 Jianhui Qu 6 Limin Guo 7 Dexiao Du 2 4 5 Xiao Jin 1 9 Yuhao Zhang 1 9 Yun Gao 1 Jie Shen 1 10 Hao Ge 1 9 Fuchou Tang 1 8 10 Yanyi Huang 1 10 11 and Jirun Peng 2 4 5 1 Biodynamic Optical Imaging Center BIOPIC College of Life Sciences Peking University Beijing 100871 China 2 Department of Surgery Beijing Shijitan Hospital Capital Medical University Beijing 100038 China 3 Department of Hepatobiliary Surgery Peking University People s Hospital Beijing 100044 China 4 Ninth School of Clinical Medicine Peking University Beijing 100044 China 5 School of Oncology Capital Medical University Beijing 100069 China 6 Center of Therapeutic Research for Liver Cancer Beijing 302 Hospital Beijing 100039 China 7 Department of Hepatobiliary Surgery Beijing DiTan Hospital Capital Medical University Beijing 100015 China 8 Ministry of Education Key Laboratory of Cell Proliferation and Differentiation College of Life Sciences Peking University Beijing 100871 China 9 BIMCR Peking University Beijing

    Original URL path: http://www.cell-research.com/arts.asp?id=2195 (2016-02-14)
    Open archived version from archive



  •