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  • Cell Research

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    Original URL path: /artsmore1.asp?id=120 (2016-02-14)



  • Cell Research
    to limit the lifespan of human cells and elicit a signal for the onset of cellular senescence To continually proliferate across the senescent checkpoint cells must restore and preserve telomere length This can be achieved by telomerase which has the reverse transcriptase activity Telomerase activity is negative in human normal somatic cells but can be detected in most tumor cells The enzyme is proposed to be an essential factor in

    Original URL path: http://www.cell-research.com/arts.asp?id=1368 (2016-02-14)
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  • Cell Research
    Wei ZHANG Hui Tu LIU The Key Laboratory of Cell Proliferation and Regulation Biology of Ministry of Education College of Life Sciences Beijing Normal University Beijing 100875 China MAPK families play an important role in complex cellular programs like proliferation differentiation development transformation and apoptosis At least three MAPK families have been characterized extracellular signal regulated kinase ERK Jun kinase JNK SAPK and p38 MAPK The above effects are fulfilled

    Original URL path: http://www.cell-research.com/arts.asp?id=1369 (2016-02-14)
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  • Cell Research
    Chris KIANI 1 Liwen CHEN 1 Yao Jiong WU 1 Albert J YEE 2 Burton B YANG 1 Sunnybrook and Women s College Health Sciences Centre and 1 Department of Laboratory Medicine and Pathobiology 2 Department of Surgeny Faculty of Medicine University of Toronto Canada Aggrecan is the major proteoglycan in the articular cartilage This molecule is important in the proper functioning of articular cartilage because it provides a hydrated gel structure via its interaction with hyaluronan and link protein that endows the cartilage with load bearing properties It is also crucial in chondroskeletal morphogenesis during development Aggrecan is a multimodular molecule expressed by chondrocytes Its core protein is composed of three globular domains G1 G2 and G3 and a large extended region CS between G2 and G3 for glycosaminoglycan chain attachment G1 comprises the amino terminus of the core protein This domain has the same structural motif as link protein Functionally the G1 domain interacts with hyaluronan acid and link protein forming stable ternary complexes in the extracellular matrix G2 is homologous to the tandem repeats of G1 and of link protein and is involved in product processing G3 makes up the carboxyl terminus of the core protein It

    Original URL path: http://www.cell-research.com/arts.asp?id=1370 (2016-02-14)
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  • Cell Research
    Yuan Chang YAN Lab of Molecular Cell Biology Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai 200031 China The isoform type of g subunits of GABA A receptor is a molecular determinant of its pharmacological characteristics At present the existence of GABA A receptor in mammalian sperm is still a controversy By using degenerate primers designed according to highly conserved region in all three g g 1 g 2 and g 3 subunits cloned in rat brain we performed reverse transcription polymerase chain reaction RT PCR to examine the expression pattern of g subunits of GABA A receptor in rat testis Only one 370 bp fragment was obtained from RT PCR in rat testis and sequencing results showed that it represented g 1 subunit but not g 2 or g 3 subunit Using the cloned fragment as probe a 3 8 kb transcript which in size as same as g 1 subunit in rat brain was detected in rat testis mRNA by performing Northern blot assay Furthermore results of in situ hybridization assay confirmed that g 1 subunit was expressed in round spermatids and spermatozoa maybe also in secondary spermatocyte These evidences

    Original URL path: http://www.cell-research.com/arts.asp?id=1371 (2016-02-14)
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  • Cell Research
    genes in human uterine leiomyomas using differential display Bin LI 1 Mei SUN 1 Bin HE 1 Jin YU 2 You Duan ZHANG 1 Yong Lian ZHANG 1 1 State Key Laboratory of Molecular Biology Institute of Biochemistry and Cell Biology Shanghai Institute for Biological Science Chinese Academy of Sciences 320 Yue Yang Road Shanghai 200031 China 2 OB GYN Hospital Shanghai First Medical University 419 Fang Xie Road Shanghai China In searching of differentially expressed genes in human uterine leiomyomas differential display was used with twelve pairs of primers to compare human uterine leiomyomas with matched myometrium False positives were eliminated by reverse Northern analysis Positives were confirmed by Northern blot analysis RESULTS Four of 69 cDNA fragments 3 up regulated named L1 L2 and L3 and 1 down regulated named M1 in leiomyoma were confirmed by Northern analysis 2 Sequence comparison and Northern analysis proved that L1 is exactly the human ribosomal protein S19 3 It was present ubiquitously in 13 tissues tested but in various levels and even in different size 4 L1 was highly expressed in parotidean cystadenocarcinoma pancreatic cancer and breast cancer examined No mutations have been found in human uterine leiomyomas n 6 CONCLUSIONS

    Original URL path: http://www.cell-research.com/arts.asp?id=1372 (2016-02-14)
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  • Cell Research
    Biological Sciences Chinese Academy of Sciences 320 Yueyang Road Shanghai 200031 China A monoclonal antibody LC 1 recognizing lung cancer associated common antigens was obtained in authors laboratory Its single chain Fv fragment ScFv named LC 1 ScFv was constructed based on recombinant phage displayed techniques For expression on cell membrane LC 1 ScFv was cloned into pDisplay vector which directed the cloned gene to express as cell membrane bound protein The resulting plasmid was sequenced and then introduced by the lipofectin method into a lung adenocarcinoma cell line SPC A 1 G418 resistant cells were obtained by G418 selection After transfection LC 1 ScFv expression was observed by Western blot analysis and the expression of cognate antigens was down regulated as shown in ELISA assay SPC A 1 pDisplay ScFv cells grew in vitro at lower speed than the control intact cells and the cells transfected with vacant vector Flow cytometry analysis detected a substantial increase in G 1 phase and decrease in S phase in population of SPC A 1 pDisplay ScFv cells compared to SPC A 1 and SPC A 1 pDisplay cells Semi quantitative RT PCR analysis showed that c myc expression was down regulated in

    Original URL path: http://www.cell-research.com/arts.asp?id=1373 (2016-02-14)
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  • Cell Research
    Sciences the Chinese Academy of Sciences Shanghai 200031 China Telomerase activity was inhibited in a dose and time dependent manner with the treatment of cisplatin for 24 48 or 72 h in a concentration ranged from 0 8 to 50 μM in BEL 7404 human hepatoma cells There were no changes in expression pattern of three telomerase subunits its catalytic reverse transcriptase subunit hTERT its RNA component hTR or the

    Original URL path: http://www.cell-research.com/arts.asp?id=1374 (2016-02-14)
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