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  • Cell Research
    Tianjin 300071 China The origin and evolution of B chromosomes could be explained by the specific DNA sequence on them But the specific sequences known were quite limited To investigate maize B chromosome sqicific DNA sequeces maize genomes with and without B chromosomes were analyzed by AFLP Only 5 markers were found specific to genomes with B chromosomes among about 2000 AFLP markers Southern hybridization and sequence analysis revealed that

    Original URL path: http://www.cell-research.com/arts.asp?id=1375 (2016-02-14)
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  • Cell Research
    Shanghai 200032 China A full length rabbit oviductin cDNA 1909bp was cloned It consists of a 5 UTR of 52bp an open reading frame ORF of 1374bp and a 3 UTR of 483bp and has more than 80 homology with that of other mammal oviductins N terminal peptide NTP 384 residues and C terminal peptide CTP 73 residues of deduced protein precursor has about 80 and 50 identity with that of other mammals respectively Fusion proteins GST NTP 368 1R 368N and GST CTP73 369F 441A were expressed and purified NH2 terminal of CTP sequencing reveals that the purified protein is consistent with the deduced one In order to study the function of NTP and CTP the mouse anti NTP and rabbit anti CTP antisera were prepared Tissue specific skeleton muscle oviduct uterus ovary liver heart and brain analysis indicated that rabbit oviductin was only found in oviduct The conditioned medium derived from the rabbit oviduct mucosa epithelial cells has a function of overcoming the early embryonic development block of Kunming mouse cultured in vitro Anti CTP antiserum could totally inhibit the early embryo development at 2 cell stage cultured in the conditioned culture medium but anti NTP antiserum couldn

    Original URL path: http://www.cell-research.com/arts.asp?id=1376 (2016-02-14)
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  • Cell Research
    its binding protein NF κB p50 in the nuclei of K562 cells Chun Hui HOU Jian HUANG Ruo Lan QIAN Group of Globin Gene Expression and Regulation State Key Labortory of Molecular Biology Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai 200031 China he developmental control of the human e globin gene expression is mediated by transcription regulatory elements in the 5 flanking DNA of this gene Sequence analysis has revealed a DNA motif GGGGAATTTGCT similar to NF k B consensus sequence resides in the negative regulatory element 3028bp 2902bp termed e NRAII 5 to the cap site of this gene NRF DNA fragment 3010bp 2986bp containing the NF k B motif similar sequence was synthesized and used in electrophoresis mobility shift assay EMSA and competitive analysis Data showed that a protein factor from nuclear extracts of K562 cells specifically interacted with NRF DNA fragment The synthetic NF DNA fragment containing NF k B consensus sequence could competed for the protein binding but MNF DNA fragment mutated NF k B motif could not suggesting that the binding protein is a member of NF k B Rel family Western blot assay demonstrated that

    Original URL path: http://www.cell-research.com/arts.asp?id=1377 (2016-02-14)
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  • Cell Research

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    Original URL path: /artsmore1.asp?id=119 (2016-02-14)


  • Cell Research
    School of Science The University of Tokyo Hongo 7 3 1 Bunkyo ku Tokyo 113 0033 Japan 2 Medical Research Laboratories Taisho Pharmaceutical Campany Ltd Yoshino cho 1 403 Ohmiya shi Saitama 330 0031 Japan 3 Department of Biochemistry Saga Medical School 5 1 1 Nabeshima Saga 849 0937 Japan We cloned cDNAs for Xenopus aldolases A B and C These three aldolase genes are localized on different chromosomes as a single copy gene In the adult the aldolase A gene is expressed extensively in muscle tissues whereas the aldolase B gene is expressed strongly in kidney liver stomach and intestine while the aldolase C gene is expressed in brain heart and ovary In oocytes aldolase A and C mRNAs but not aldolase B mRNA are extensively transcribed Thus aldolase A and C mRNAs but not B mRNA occur abundantly in eggs as maternal mRNAs and strong expression of aldolase B mRNA is seen only after the late neurula stage We conclude that aldolase A and C mRNAs are major aldolase mRNAs in early stages of Xenopus embryogenesis which proceeds utilizing yolk as the only energy source aldolase B mRNA on the other hand is expressed only later in development

    Original URL path: http://www.cell-research.com/arts.asp?id=1359 (2016-02-14)
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  • Cell Research
    by epidermal powder immunization Dexiang CHEN Lendon G PAYNE PowderJect Vaccines Inc 585 Science Drive Madison WI 53711 USA Immune reactions to foreign or self antigens lead to protective immunity and sometimes immune disorders such as allergies and autoimmune diseases Antigen presenting cells APC including epidermal Langerhans cells LCs play an important role in the course and outcome of the immune reactions Epidermal powder immunization EPI is a technology that

    Original URL path: http://www.cell-research.com/arts.asp?id=1360 (2016-02-14)
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  • Cell Research
    USHER 2 Li Q ZHANG 3 1 Division of Pulmonary and Critical Care Medicine Johns Hopkins University School of Medicine Baltimore MD 21224 USA 2 Department of Biological Science University of Delaware Newark DE 19716 USA 3 Department of Surgery University of Maryland Baltimore MD 21201 USA The wealth of DNA data generated by the human genome project coupling with recently invented high throughput gene expression profiling techniques has dramatically sped up the process for biomedical researchers on elucidating the role of genes in human diseases One powerful method to reveal insight into gene functions is the systematic analysis of gene expression Two popular high throughput gene expression technologies microarray and Serial Analysis of Gene Expression SAGE are capable of producing large amounts of gene expression data with the potential of providing novel insights into fundamental disease processes especially complex syndromes such as cardiovascular disease whose etiologies are due to multiple genetic factors and their interplay with the environment Microarray and SAGE have already been used to examine gene expression patterns of cell culture animal and human tissues models of cardiovascular diseases In this review we will first give a brief introduction of microarray and SAGE technologies and point out

    Original URL path: http://www.cell-research.com/arts.asp?id=1361 (2016-02-14)
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  • Cell Research
    Free Sample Issue Submission Advanced Online Publication Current Issue Top 10 VOLUME 12 ISSUE 2 6 2002 117 121 Cytopathological evaluations combined RNA and protein analyses on defined cell regions using single frozen tissue block Hong LI Xiao Yan CHEN Qing You KONG Jia LIU Laboratory of Cell Biology and Molecular Genetics College of Basic Medical Sciences Dalian Medical University Dalian 116027 China The co existence of multiple cell components in tissue samples is the main obstacle for precise molecular evaluation on defined cell types Based on morphological examination we developed an efficient approach for paralleled RNA and protein isolations from an identical histological region in frozen tissue section The RNA and protein samples prepared were sufficient for RT PCR and Western blot analyses and the results obtained were well coincident each other as well as with the corresponding parameters revealed from immunohistochemical examinations By this way the sampling problem caused by cell cross contamination can be largely avoided committing the experimental data more specific to a defined cell type These novel methods thus allow us to use single tissue block for a comprehensive study by integration of conventional cytological evaluations with nucleic acid and protein analyses keywords RNA and

    Original URL path: http://www.cell-research.com/arts.asp?id=1362 (2016-02-14)
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