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  • Cell Research
    200090 China Correspondence Zhi Hong ZHANG Tel 86 21 65643673 E mail zhzhang fudan edu cn Swelling activated Cl currents I Cl swell were measured during hyposmotic shock in white Leghorn embryonic chick heart cells using the whole cell recording of patch clamp technique Genistein an inhibitor of protein tyrosine kinase PTK suppressed I Cl swell Under isosmotic condition phorbol 12 myristate 13 acetate PMA an activator of PKC elicited the Cl current similar to that in hyposmotic solution whereas hyposmotic shock did not elicit I Cl swell in chelerythrine chloride an inhibitor of PKC treated cells Confocal microscopy experiments using FITC phalloidin as a fluorescent label of F actin showed that the actin network was moved from cortical region of the cell to the center after hyposmotic shock as compared with the image under isosmotic condition When the cells were treated with cytochalasin B CB or cytochalasin D CD under isosmotic condition the disruption of the F actin integrity was observed and I Cl swell was not elicited With combination treatment of CB with PMA hyposmotic solution could not elicited I Cl swell The results suggested that the role of PTK probably receptor tyrosine kinase for regulation of I

    Original URL path: http://www.cell-research.com/arts.asp?id=1332 (2016-02-14)
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  • Cell Research
    DONG Liang Wei LI Jing WANG Chao Chen WANG Jing LIU School of Life Science University of Science and Technology of China Hefei 230027 China Correspondence Jing LIU Tel 0086 551 3606294 E mail charlson mail ustc edu cn The c erbB 2 proto oncogene encodes a 185kDa protein p185 which belongs to epidermal growth factor receptor family Amplification of this gene has been shown to correlate with poor clinical prognosis for certain cancer patients The monoclonal antibody A21 which directed against p185 specifically inhibits proliferation of tumor cells overexpressing p185 hence allows it to be a candidate for targeted therapy In order to overcome several drawbacks of murine MAb we cloned its V H and V L genes and constructed the single chain Fv scFv through a peptide linker The recombinant scFvA21 was expressed in Escherichia coli and purified by the affinity column Subsequently it was characterized by ELISA Western blot cell immunohistochemistry and FACS All these assays showed the binding activity to extracellular domain ECD of p185 Based on those properties of scFvA21 we further constructed the scFv Fc fusion molecule with a homodimer form and the recombinant product was expressed in mammalian cells In a series of

    Original URL path: http://www.cell-research.com/arts.asp?id=1334 (2016-02-14)
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  • Cell Research
    of Natural Science Chungnam National University 220 Gung dong Yuseong gu Daejeon 305 764 Korea 3 Department of Food and Nutrition Hannam University Daejeon 306 791 Korea 4 Institute for Virology and Immunobiology University of Wuerzburg Versbacher Str 7 97078 Wuerzburg Germany Correspondence Kwan Hee YOU Tel 0082 42 821 5498 E mail khyou cnu ac kr Clusterin is a 75 80 kDa heterodimeric glycoprotein that is produced in most tissues but which exact biological role is still not clear Particularly its role in protection or promotion of apoptosis is heavily disputed since data supporting both views have been reported in several independent studies To clarify this issue and also to determine whether clusterin expression itself might be affected by apoptosis in the present study rat thymocytes were treated with dexamethasone a synthetic glucocorticoid that elicits apoptosis in thymocytes and clusterin mRNA expression was analyzed by semi quantitative RT PCR before and after induction of apoptosis Interestingly neither the treatment with dexamethasone in vitro nor triggering of apoptosis in vivo up regulated clusterin expression opposing the view that clusterin is involved in apoptotic processes On the other hand a new clusterin mRNA isoform was detected and isolated whose expression was

    Original URL path: http://www.cell-research.com/arts.asp?id=1335 (2016-02-14)
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  • Cell Research
    Molecular Genetics Institute of Plant Physiology and Ecology Shanghai Institutes for Biological Science SIBS Chinese Academy of Sciences and Partner Group of Max Planck Institute of Molecular Plant Physiology MPI MP on Plant Molecular Physiology and Signal Transduction 300 Fenglin Road 200032 Shanghai China Correspondence These authors contribute equally to the paper Tel 0086 21 64042090 ext 4411 E mail hwxue iris sipp ac cn Phytohormone abscisic acid ABA was critical for many plant growth and developmental processes including seed maturation germination and response to environmental factors With the purpose to detect the possible ABA related signal transduction pathways we tried to isolate ABA regulated genes through cDNA macroarray technology using ABA treated rice seedling as materials under treatment for 2 4 8 and 12 h Of 6144 cDNA clones tested 37 differential clones showing induction or suppression for at least one time were isolated Of them 30 and 7 were up or down regulated respectively Sequence analyses revealed that the putative encoded proteins were involved in different possible processes including transcription metabolism and resistance photosynthesis signal transduction and seed maturation 6 cDNA clones were found to encode proteins with unknown functions Regulation by ABA of 7 selected clones relating

    Original URL path: http://www.cell-research.com/arts.asp?id=1336 (2016-02-14)
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  • Cell Research

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    Original URL path: /artsmore1.asp?id=115 (2016-02-14)


  • Cell Research
    Sample Issue Submission Advanced Online Publication Current Issue Top 10 VOLUME 13 ISSUE 2 4 2003 69 81 Function and regulation of Aurora Ipl1p kinase family in cell division Yu Wen KE 1 Zhen DOU 1 Jie ZHANG 1 Xue Biao YAO 1 2 1 Laboratory for Cell Dynamics School of Life Sciences University of Science and Technology of China Hefei 230027 China 2 Department of Molecular and Cell Biology University of California Berkeley CA 94720 USA Correspondence Xue Biao YAO Tel 0086 551 3607141 E mail yaoxb ustc edu cn During mitosis the parent cell distributes its genetic materials equally into two daughter cells through chromosome segregation a complex movements orchestrated by mitotic kinases and its effector proteins Faithful chromosome segregation and cytokinesis ensure that each daughter cell receives a full copy of genetic materials of parent cell Defects in these processes can lead to aneuploidy or polyploidy Aurora Ipl1p family a class of conserved serine threonine kinases plays key roles in chromosome segregation and cytokinesis This article highlights the function and regulation of Aurora Ipl1p family in mitosis and provides potential links between aberrant regulation of Aurora Ipl1p kinases and pathogenesis of human cancer keywords Aurora Ipl1p mitosis

    Original URL path: http://www.cell-research.com/arts.asp?id=1324 (2016-02-14)
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  • Cell Research
    Cloning and characterization of human IC53 2 a novel CDK5 activator binding protein Yi Hu XIE 1 Xiang Huo HE 1 Yun Tian TANG 2 Jin Jun LI 1 Zhi Mei PAN 1 Wen Xin QIN 1 Da Fang WAN 1 Jian Ren GU 1 1 State Key Laboratory of Oncogenes and Related Genes Shanghai Cancer Institute Shanghai 200032 China 2 Peopele s Hospital of Guangxi Zhuang Autonomous Region Nanning 530021 China Correspondence Da Fang WAN Jian Ren GU Tel 0086 21 64177401 E mail nlorg public sta net cn We have identified IC53 2 a human homologue of the rat C53 gene from a human placenta cDNA library GeneBank Accession No AF217982 IC53 2 can bind to the CDK5 activator p35 by in vitro association assay IC53 2 is mapped to human chromosome 17q21 31 The IC53 2 transcript is highly expressed in kidney liver skeletal muscle and placenta It is abundantly expressed in SMMC 7721 C 33A 3AO A431 and MCF 7 cancer cell lines by RT PCR assay Stable transfection of IC53 2 cDNA into the hepatocellular carcinoma SMMC 7721 cell remarkably stimulates its growth in vitro The above results indicate that IC53 2 is a novel

    Original URL path: http://www.cell-research.com/arts.asp?id=1325 (2016-02-14)
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  • Cell Research
    levels of transcription Ling CAI Jing De ZHU Cancer Genetics and Gene Therapy The State key Laboratory for Oncogenes and Related Genes Shanghai Cancer Institute Shanghai Jiao Tong University LN 25 2200 Xie tu Road Shanghai 200032 China Correspondence Jing De ZHU Tel 0086 21 64224285 E mail zhujingde yahoo com The tumor selective over expression of the human Hsp70 gene has been well documented in human tumors linked to the poor prognosis being refractory to chemo and radio therapies as well as the advanced stage of tumorous lesions in particular However both the nature and details of aberrations in the control of the Hsp70 expression in tumor remain enigmatic By comparing various upstream segments of the Hsp70 gene for each s ability to drive the luciferase reporter genes in the context of the tumor cell lines varying in their p53 status and an immortal normal liver cell line we demonstrated in a great detail the defects in the control mechanisms at the both initiation and elongation levels of transcription being instrumental to the tumor selective profile of its expression Our data should not only offer new insights into our understanding of the tumor specific over expression of the human

    Original URL path: http://www.cell-research.com/arts.asp?id=1326 (2016-02-14)
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