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  • Cell Research
    138 Yi Xue Yuan Road Shanghai 200032 China 2 Department of Hematology the Affiliated Xiehe Hospital Tongji Medical School Huazhong University ofScience and Technology 1277 Jiefang Avenue Wuhan 430022 China 3 Department of Molecular Oncology Cancer Research Institute Kanazawa University Takara machi 13 1 Kanazawa 920 0934 Japan Present address Department of Biochemistry University of Medicine and Dentistry of New Jersey Robert Wood Johnson Medical School Piscataway NJ 08854 USA Tel 001 732 235 4194 E mail weiwe umdnj com Correspondence Seishi MURAKAMI Tel 0081 76 265 2731 E mail semuraka kenroku ipc kanazawa u ac jp RMP was reported to regulate transcription via competing with HBx to bind the general transcription factor IIB TFIIB and interacting with RPB5 subunit of RNA polymerase II as a corepressor of transcription regulator However our present research uncovered that RMP also regulates the transcription through interaction with the general transcription factors IIF TFIIF which assemble in the preinitiation complex and function in both transcription initiation and elongation With in vitro pull down assay and Far Western analysis we demonstrated that RMP could bind with bacterially expressed recombinant RAP30 and RAP74 of TFIIF subunits In the immunoprecipitation assay in COS1 cells cotransfected with FLAG tagged RMP or its mutants GST fused RAP30 and RAP74 were co immunoprecipitated with RMP in approximately equal molar ratio which suggests that RAP30 and RAP74 interact with RMP as a TFIIF complex Interestingly both RAP30 and RAP74 interact with the same domain D5 of the C terminal RMP of 118 amino acid residuals which overlaps with its TFIIB binding domain Internal deletion of D5 region of RMP abolished its binding ability with both subunits of TFIIF while D5 domain alone was sufficient to interact with TFIIF subunits The result of luciferase assay showed that overexpression of RMP but not

    Original URL path: http://www.cell-research.com/arts.asp?id=1327 (2016-02-14)
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  • Cell Research
    Sciences Chinese Academy of Sciences Shanghai 200031 China 2 The Population Council Center for Biomedical Research 1230 York Avenue New York New York 100021 USA Correspondence Yuan Chang YAN Tel 86 21 54921395 E mail ycyan sunm shcnc ac cn Mouse sp56 is considered as one of the candidates for mouse zona pellucida 3 mZP3 receptor Up to date its homologue has only been cloned from guinea pig namely AM67 Based on the cDNA sequence of mouse sp56 we designed a pair of primer to amplify its homologue from rat testis cDNA Using RT PCR two fragments of 743 bp and 938 bp were amplified The PCR products show very high homology to mouse sp56 However the 743 bp product completely lacks one of the seven Sushi domains of mouse sp56 Using the 743 bp product as the probe to detect the expression profile of rat sp56 in rat tissues Northern blot shows that a 2 0 kb mRNA expresses specifically in testis Employed the RACE method two full cDNA sequences of rat sp56 were obtained A Mr 42 KD band was detected in denatured and non reducing protein sample of rat testis and sperm with anti mouse sp56 monoclonal

    Original URL path: http://www.cell-research.com/arts.asp?id=1328 (2016-02-14)
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  • Cell Research
    Biological Science SIBS The Chinese Academy of Sciences Partner Group el Max Planck Institute of Molecular Plant Physiology MPI MP on Plant Molecular Physiology and Signal Transduction 300 Fenglin Road 200032 Shanghai China Correspondence Hong Wei XUE Tel 0086 21 64042090 ext 4411 E mail hwxue iris sipp ac cn A partial rice Oryza sativa L cDNA clone OsPI4K1c was isolated through screening of a cDNA library constructed from tillering materials OsPI4Klc encoded a peptide of 608 amino acids with a calculated molecular mass of 68 4 kDa The OsPI4Klc peptide shared high homology and possessed the highly conserved domains present in most isolated cloned P14 kinases i e a lipid kinase unique LKU domain and a catalytic CAT domain A region with similarity to pleckstrin homology PH domain was present in OsPI4Klc as well Further comparison with genomic sequences in databases revealed that OsPI4K1c is located at the 3 end of a putative rice PI 4 kinase coding gene OsPI4K1 and its coding region corresponded to the C terminal half of OsPI4K1 protein Twelve exons 49 562 bp in size and 11 introns 77 974 bp in size were identified in OsPI4K1c The recombinant protein expressed in Escherichia coli

    Original URL path: http://www.cell-research.com/arts.asp?id=1329 (2016-02-14)
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  • Cell Research

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    Original URL path: /artsmore1.asp?id=113 (2016-02-14)


  • Cell Research
    1 Wei LU 1 Tie Liu SHI 1 Jin WANG 2 Hong Xia WANG 2 Hua Liang JIANG 1 Jian Hua SHEN 1 You Hua XIE 1 Yuan WANG 1 Gang PEI 1 Bei Fen SHEN 2 Jia Rui WU 1 Bing SUN 1 1 Institute of Biochemistry and Cell Biology Institute of Materia Medica Bioinformation Center Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai 200031 China 2 Institute of Microbiology and Epidemiology Institute of Basic Medical Sciences Academy of Military Medical Sciences Beijing 100071 China Correspondence Jia Rui WU Bing SUN Tel 0086 21 54921376 E mail wujr sunm shcnc ac cn bsun sibs ac cn The nucleocapsid N protein of severe acute respiratory syndrome coronavirus SARS CoV is a major virion structural protein In this study two epitopes N1 and N2 of the N protein of SARS CoV were predicted by bioinformatics analysis After immunization with two peptides the peptides specific antibodies were isolated from the immunized rabbits The further experiments demonstrated that N1 peptide induced polyclonal antibodies had a high affinity to bind to E coli expressed N protein of SARS CoV Furthermore it was confirmed that N1 peptide specific IgG antibodies were detectable in

    Original URL path: http://www.cell-research.com/arts.asp?id=1316 (2016-02-14)
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  • Cell Research
    Fang GUI State Key Laboratory of Freshwater Ecology and Biotechnology Wuhan Center for Developmental Biology Institute of Hydrobiology Chinese Academy of Sciences Wuhan 430072 China Correspondence Jian Fang GUI E mail jfgui ihb ac cn A cell free system based upon the egg extracts from gynogenetic gibel carp Carassius auratus gibelio or bisexual red common carp Cyprinus carpio red variety was developed to investigate developmental behaviors of the demembranated sperm nuclei Both red common carp and gibel carp sperm nuclei could decondense fully and form pronuclei in the red common carp egg extracts Gibel carp sperm nuclei could also decondense fully and form pronuclei in the gibel carp egg extracts but red common carp sperm nuclei could not decondense sufficiently in the same extracts The significant differences of morphological changes were further confirmed by ultrastructural observation of transmission electron microscopy The data further offer cytological evidence for gonochoristic reproduction in the gynogenetically reproducing gibel carp In addition the sperm nuclei in vitro decondensation is dependent on the pH in the extracts and the decondensed efficiency is optimal at pH 7 However no DNA replication was observed in the two kinds of egg extracts during the incubation period of the sperm

    Original URL path: http://www.cell-research.com/arts.asp?id=1318 (2016-02-14)
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  • Cell Research
    Shi Ying MIAO 1 Shu Dong ZONG 2 Qi SHENG 1 Lin Fang WANG 1 1 National Laboratory of Medical Molecular Biology Institute of Basic Medical Sciences Chinese Academy of Medical Sciences Peking Union Medical College 5 Dong Dan 3 tiao Beijing 100005 China 2 National Research Institute for Family Planing WHO Collaboration Center for Research in Human Reproduction 12 Da Hui Si Beijing 100081 China Correspondence Lin Fang WANG Tel 0086 10 65296418 E mail wanglf ms imicams ac cn Variable Charge X Y VCX Y is a human testis specific gene family that localized on X and Y chromosomes In this study VCY protein was expressed in E coli in the form of glutathione S transferase GST fusion protein With the purified fusion protein as antigen the anti GST VCY antibody was generated and the localization of VCY protein in human testis was determined by immunohistochemistry In the testis seminiferous epithelium VCY proteins were highly expressed in nuclei of germ cells Using propidium iodide staining and green fluorescent protein GFP tag technologies VCY and VCX 8r proteins were mainly localized in the nucleoli of COS7 cells In addition the colocalization for VCY and VCX 8r in COS7 cells

    Original URL path: http://www.cell-research.com/arts.asp?id=1319 (2016-02-14)
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  • Cell Research
    China Normal University 200062 Shanghai China Correspondence Yi Ping LI Tel 0086 21 54921415 E mail oocyte sunm shcnc ac cn One cell mouse embryos from KM strain and B6C3F1 strain were cultured in M16 medium in which 2 cell block generally occurs Embryos of KM strain exhibited 2 cell block whereas B6C3F1 embryos which are regarded as a nonblocking strain proceeded to the 4 cell stage in our culture condition It is often assumed that the block of early development is due to the failure of zygotic gene activation ZGA in cultured embryos In this study we examined protein synthesis patterns by two dimensional gel electrophoresis of 35S methionine radiolabeled 2 cell embryos Embryos from the blocking strain and the nonblocking strain were compared in their development both in vitro and in vivo The detection of TRC expression a marker of ZGA at 42 h post hCG in KM embryos developed in vitro suggested that ZGA was also initiated even in the 2 cell arrested embryos Nevertheless a significant delay of ZGA was observed in KM strain as compared with normally developed B6C3F1 embryos At the very beginning of major ZGA as early as 36 h post hCG TRC

    Original URL path: http://www.cell-research.com/arts.asp?id=1320 (2016-02-14)
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