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  • Cell Research
    cells insights from an ultrastructural study Zhen DOU 1 Akira SAWAGECHI 2 Jie ZHANG 1 Hong LUO 1 Lawrence BRAKO 3 Xue Biao YAO 1 2 1 Laboratory of Cell Dynamics School of Life Sciences University of Science and Technology of China Hefei 230027 China 2 Department of Molecular and Cell Biology University of California Berkeley CA 94720 USA 3 Electron Microscopy Facility Morhouse School of Medicine Atlanta GA 30310 USA These two authors contributed equally to this work Correspondence Xue Biao YAO Tel 551 360 7141 E mail yaoxb ustc edu cn Entry into mitosis is driven by signaling cascades of mitotic kinases Our recent studies show that TTK a kinetochore associated protein kinase interacts with CENP E a mitotic kinesin located to corona fiber of kinetochore Using immunoelectron microscopy here we show that TTK is present at the nuclear pore adjacent complex of interphase HeLa cells Upon nuclear envelope fragmentation TTK targets to the outermost region of the developing kinetochores of monoorient chromosome as well as to spindle poles After stable attachment throughout chromosome congression TTK is a constituent of the corona fibers extending up to 90 nm away from the kinetochore outer plate Upon metaphase alignment TTK

    Original URL path: http://www.cell-research.com/arts.asp?id=1287 (2016-02-14)
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  • Cell Research
    Mei LI 1 Benoit VIOLLET 2 You Hua XIE 1 Yuan WANG 1 1 State Key Laboratory of Molecular Biology Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai China E mail wangy sibs ac cn 2 Oncogenic Viruses Unit Department of Biotechnology Institut Pasteur Paris France Current address Institut Cochin Departement GDPM Paris France Current address I G B M C I rue Laurent Fries B P 163 Illkirch CEDEX Strasbourg France Correspondence Yuan Wang Tel 86 21 54921103 E mail wangy sibs ac cn yhxie sibs ac cn Enhancer II ENII is one of the critical cis elements in the Hepatitis B Virus HBV genome for the hepatic viral gene transcription and DNA replication The liver specific activity of ENII is regulated by multiple liver enriched transcription factors including LRH 1 hB1F HNF1 HNF3b HNF4 and C EBP Knowledge on the interplay of these important factors is still limited In this study we demonstrate a functional synergism between the orphan nuclear receptor LRH 1 hB1F and the homeoprotein HNF1 in up regulating the liver specific activity of ENII This synergism is sufficient for initiating the viral gene transcription and DNA replication

    Original URL path: http://www.cell-research.com/arts.asp?id=1288 (2016-02-14)
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  • Cell Research
    metamorphosis indu D PAUL Yun Bo SHI Laboratory of Gene Regulation and Development National Institute of Child Health and Human Development National Institutes of Health Bldg USA Correspondence Yun Bo SHI Tel 301 402 1004 E mail shi helix nih gov The biological effects of thyroid hormone T 3 are mediated by the thyroid hormone receptor TR Amphibian metamorphosis is one of the most dramatic processes that are dependent on T 3 T 3 regulates a series of orchestrated developmental changes which ultimately result in the conversion of an aquatic herbivorous tadpole to a terrestrial carnivorous frog T 3 is presumed to bind to TRs which in turn recruit coactivators leading to gene activation The best studied coactivators belong to the p160 or SRC family Members of this family include SRC1 NCoA 1 SRC2 TIF2 GRIP1 and SRC3 pCIP ACTR AIB 1 RAC 3 TRAM 1 These SRCs interact directly with liganded TR and function as adapter molecules to recruit other coactivators such as p300 CBP Here we studied the expression patterns of these coactivators during various stages of development Amongst the coactivators cloned in Xenopus laevis SRC3 was found to be dramatically upregulated during natural and T 3 induced

    Original URL path: http://www.cell-research.com/arts.asp?id=1289 (2016-02-14)
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  • Cell Research
    unloading distinct mechanisms in the spleen and thymus Li Xin WEI Jian Nian ZHOU Arthur I ROBERTS Yu Fang SHI Department of Molecular Genetics Microbiology and Immunology University of Medicine and Dentistry of New Jersey Robert Wood Johnson Medical School 661 Hoes Lane Piscataway New Jersey 08854 USA Correspondence Yufang SHI Tel 732 235 4501 E mail yufang shi umdnj edu Hindlimb unloading HU in rodent is a well accepted ground based model used to simulate some of the conditions of space flight and reproduce its deleterious effects on the musculoskeletal cardiovascular and immune systems In this study the effects of HU on lymphocyte homeostasis in the spleen and thymus of mice were examined HU was found to drastically deplete various cell populations in the spleen and thymus These changes are likely to be mediated by apoptosis since DNA strand breaks indicative of apoptosis were detected by terminal deoxynucleotidyl transferase mediated nick end labeling in both splenocytes and thymocytes Surprisingly administration of opioid antagonists or interference with the Fas FasL interaction was able to block HU induced reductions of splenocytes but not thymocytes On the other hand steroid receptor antagonists blocked the reduction of lymphocyte numbers in both spleen and

    Original URL path: http://www.cell-research.com/arts.asp?id=1290 (2016-02-14)
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  • Cell Research
    Jie CAO 1 Ping ZHAO 1 Xiao Hui MIAO 2 Lan Juan ZHAO 1 Li Jun XUE 1 Zhong Tian QI 1 1 Department of Microbiology Second Military Medical University Shanghai 200433 China 2 Department of Infectious Diseases Changzheng Hospital Second Military Medical University Shanghai 200003 China Correspondence Zhong Tian QI Tel 0086 21 25070312 E mail qizt smmu edu cn The human CD81 hCD81 the most recently proposed receptor of hepatitis C virus HCV can especifically bind to HCV envelope glycoprotein 2 E2 In this study hCD81 expressing murine NIH 3T3 cells were used to select hCD81 binding peptides from a phage displayed nonapeptide library PVIII9aaCys Eighteen of the 75 clones selected from the library showed specific binding to the hCD81 expressing NIH 3T3 cells by enzyme linked immunosorbent assay ELISA and competitive inhibition test Twelve out of the 18 clones shared the amino acid motif SPQYWTGPA Sequence comparison of the motif showed no amino acid homology with the native HCV E2 The motif containing phages could competitively inhibit the ability of HCV E2 binding to native hCD81 expressing MOLT 4 cells and induce HCV E2 specific immune response in vivo These results suggest that the selected motif SPQYWTGPA

    Original URL path: http://www.cell-research.com/arts.asp?id=1291 (2016-02-14)
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  • Cell Research
    Hui WANG 1 Lan Ying SUN 1 Qi Jun QIAN 2 Xin Yuan LIU 1 1 Laboratory of Biotechnology Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai 200031 China E mail xyliu sibs ac cn 2 Eastern Heptobiliary Hospital Second Military Medical University Shanghai 200438 China Correspondence Xin Yuan LIU Tel 86 21 64746126 E mail xyliu sibs ac cn ONYX 015 is an attractive therapeutic adenovirus for cancer because it can selectively replicate in tumor cells and kill them To date clinical trials of this adenovirus have demonstrated marked safety but not potent enough when it was used alone In this paper we put forward a novel concept of Gene ViroTherapy strategy and in this way we constructed an armed therapeutic oncolytic adenovirus system ZD55 gene which is not only deleted of E1B 55 kD gene similar to ONYX 015 but also armed with foreign antitumor gene ZD55 gene exhibited similar cytopathic effects and replication kinetics to that of ONYX 015 in vitro Importantly the carried gene is expressed and the expression level can increase with the replication of virus Consequently a significant antitumoral efficacy was observed when ZD55 CD 5

    Original URL path: http://www.cell-research.com/arts.asp?id=1292 (2016-02-14)
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  • Cell Research
    Zoology the Chinese Academy of Sciences Kunming Yunnan 650019 China E mail zhangyp public km yn cn 2 Laboratory for Conservation and Utilization of Bio resource Yunnan University Kunming Yunnan 650223 China 3 Biological Laboratory Sapporo College Hokkaido University of Education Sapporo 002 8502 Japan E mail watabe sap hokkyodai ac jp 4 Graduate School of the Chinese Academy of Sciences Beijing China 5 Department of Biology Tokyo Metropolitan University Hachioji shi 192 0397 Japan Correspondence Ya Ping Zhang Hide aki WATABE Tel 0871 5190761 0081 11 778 0342 E mail zhangyp public km yn cn watabe sap hokkyodai ac jp Hindlimb unloading HU in rodent is a well accepted ground based model used to simulate some of the conditions of space flight and reproduce its deleterious effects on the musculoskeletal cardiovascular and immune systems In this study the effects of HU on lymphocyte homeostasis in the spleen and thymus of mice were examined HU was found to drastically deplete various cell populations in the spleen and thymus These changes are likely to be mediated by apoptosis since DNA strand breaks indicative of apoptosis were detected by terminal deoxynucleotidyl transferase mediated nick end labeling in both splenocytes and thymocytes Surprisingly

    Original URL path: http://www.cell-research.com/arts.asp?id=1293 (2016-02-14)
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  • Cell Research
    13 ISSUE 6 12 2003 503 507 Mortalin imaging in normal and cancer cells with quantum dot immuno conjugates Zeenia KAUL Tomoko YAGUCHI Sunil C KAUL Takashi HIRANO Renu WADHWA Kazunari TAIRA National Institute of Advanced Industrial Science and Technology AIST 1 1 1 Higashi Tsukuba Ibaraki 3058562 Japan Correspondence Renu WADHWA Tel 81 29 861 9464 E mail renu wadhwa aist go jp Quantum dots are the nanoparticles that are recently emerging as an alternative to organic fluorescence probes in cell biology and biomedicine and have several predictive advantages These include their i broad absorption spectra allowing visualization with single light source ii exceptional photo stability allowing long term studies and iii narrow and symmetrical emission spectrum that is controlled by their size and material composition These unique properties allow simultaneous excitation of different size of quantum dots with a single excitation light source their simultaneous resolution and visualization as different colors At present there are only a few studies that have tested quantum dots in cellular imaging We describe here the use of quantum dots in mortalin imaging of normal and cancer cells Mortalin staining pattern with quantum dots in both normal and cancer cells mimicked those obtained

    Original URL path: http://www.cell-research.com/arts.asp?id=1295 (2016-02-14)
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