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  • Cell Research
    Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai 200031 China 2 Department of Urology Tongji Hospital Tongji University 389 Xincun Road Shanghai 200065 China 3 New Drug R D Center North China Pharmaceutical Group Corporation 388 Heping East Road Shijia Zhuang 050015 Hebei China 4 School of life sciences Fudan University 220 Handan Road Shanghai 200433 China 5 Shanghai Research Center for Biomodel Organism Shanghai 201203 China Correspondence Li He GUO Tel 0086 21 54921392 E mail mhzhang sunm shcnc ac cn It is well documented that γ aminobutyric acid GABA system existed in reproductive organs Recent researches showed that GABA A and GABA B receptors were present in testis and sperm and might mediate the acrosome reaction induced by GABA and progesterone GABA transporter I GAT1 also existed in testis and sperm but its physiological function was unknown In the present study we used GAT1 overexpressing mice to explore GAT1 function in male reproductive system We found that the expression level of GAT1 continuously increased in wild type mouse testis from 1 month to 2 months after birth GAT1 overexpression in mouse affected testis development which embodied reduced testis mass and slowed spermatogenesis in transgenic

    Original URL path: http://www.cell-research.com/arts.asp?id=1280 (2016-02-14)
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  • Cell Research
    Zhi Qian ZHANG Peking University School of Oncology and Beijing Institute for Cancer Research Beijing 100034 China Correspondence Zhong Xiang LIN Tel 86 10 6617 9250 86 10 6617 6447 E mail zhongxianglin Yahoo com To examine the role of gap junctions in cell senescence the changes of gap junctions in cisplatin induced premature senescence of primary cultured fibroblasts were studied and compared with the replicative senescent human fibroblasts Dye transfer assay for gap junction function and immunofluorescent staining for connexin 43 protein distribution were done respectively Furthermore cytofluorimetry and DAPI fluorescence staining were performed for cell cycle and apoptosis analysis p53 gene expression level was detected with indirect immunofluorescence We found that cisplatin 10 mM treatment could block cell growth cycle at G1 and induced premature senescence The premature senescence changes included high frequency of apoptosis elevation of p53 expression loss of membranous gap junctions and reduction of dye transfer capacity These changes were comparable to the changes of replicative senescence of human fibroblasts It was also concluded that cisplatin could induce premature senescence concomitant with inhibition of gap junctions in the fibroblasts Loss of functional gap junctions from the cell membrane may account for the reduced intercellular communication

    Original URL path: http://www.cell-research.com/arts.asp?id=1281 (2016-02-14)
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  • Cell Research
    com cn We have previously shown a critical role of prolactin PRL during maturation and anti tumor effects of murine natural killer NK cells in vitro and in vivo We extended that study by exploring the ability of human NK cell lines NK 92 and YT cell to express PRL receptor PRL R and to respond to PRL stimulation in vitro Both human NK cell lines constitutively expressed PRL R on membrane and mRNA transcripts NK 92 cells contained higher level of PRL R than YT cells which correlated to the enhanced capacity of the cells to proliferate and to lyse target cells in response to PRL stimulation in the presence of trace amount of IL 2 or IL 15 in vitro Two differences between IL 2 and IL 15 in functioning on human NK cells were for the first time observed PRL synergized with IL 15 to improve proliferation of NK cells in a dose dependent manner without double peak manifesting like IL 2 Although PRL enhanced the cytotoxicity of IL 2 or IL 15 activated NK cells it exerted the function through up regulating gene expression of perforin without influence of FasL in IL 2 stimulated NK cells

    Original URL path: http://www.cell-research.com/arts.asp?id=1282 (2016-02-14)
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  • Cell Research
    cell line BEL 7402 Dong Hong ZHAO Jian Jun HONG Shi Ying GUO Run Lin YANG Jun YUAN Chuan Jun WEN Kai Ya ZHOU Chao Jun LI The Jiangsu Key Laboratory of Molecular Medical Biotechnology College of Life Science Nanjing Normal University Nanjing 210097 Jiangsu China Correspondence Chao Jun LI Tel 0086 25 3598779 E mail licj njnu edu cn Wnt signaling pathway is essential for development and tumorigenesis however this signaling pathway in the progress of hepatocellular carcinoma HCC remains unclear In this paper we studied the function of human T cell transcription factor 4 TCF4 a key factor of Wnt signaling pathway on the proliferation of HCC cell line We showed that the expression of TCF4 mRNA in HCC cell line BEL 7402 was higher than that in immortalized normal liver cell line L02 Blockage of Wnt pathway by NTCF4 a dominant negative TCF4 could suppress BEL 7402 cells growth and decrease the expression of cyclin D1 and c myc two of target genes of Wnt pathway On the other hand stimulating Wnt pathway by introducing a degradation resistant catenin S37A could increase BEL 7402 cells proliferation But all the treatments had no effect on L02 cells Our

    Original URL path: http://www.cell-research.com/arts.asp?id=1283 (2016-02-14)
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  • Cell Research
    is associated with the level of reactive oxygen species Fei GAO 1 Jing YI 1 Jing Qi YUAN 2 Gui Ying SHI 1 Xue Ming TANG 1 1 Department of Cell Biology Shanghai Second Medical University Shanghai 200025 China 2 Department of Automation Shanghai Jiaotong University Shanghai 200031 China Correspondence Xue Ming TANG Tel 86 21 63846590 ext 776480 E mail xmtang shsmu edu cn Double staining flow cytometry was performed using 7 amino actinomycin D and 6 carboxy 2 7 dichlorodihydrofluorescein diacetate to detect the level fluctuation of reactive oxygen species ROS during the cell cycle of normal NB4 cells Our results showed that NB4 cells possessed higher level of ROS in G2 M phase than in G1 and S phases Double staining flow cytometry with TdT mediated dUTP nick end labeling Tunel and propidium iodide PI indicated that As 2 O 3 2 μ M could induce apoptosis in NB4 cells prevailingly from G2 M phase and this efficacy was enhanced upon co administration of 2 3 dimethoxy 1 4 naphthoquinone DMNQ 2 5 μ M which could produce the endogenous ROS These results suggested that different ROS level in different cell cycle phases of NB4 cells might

    Original URL path: http://www.cell-research.com/arts.asp?id=1284 (2016-02-14)
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  • Cell Research
    Free Sample Issue Submission Advanced Online Publication Current Issue Top 10 VOLUME 14 ISSUE 1 2 2004 86 91 Intermediate fertile Triticum aestivum Agropyron elongatum somatic hybrids are generated by low doses of UV irradiation Ai Xia CHENG Guang Min XIA Da Ying ZHI Hui Min CHEN School of Life Sciences Shandong University Jinan 250100 Shandong China Correspondence Guang Min XIA E mail Xiagm sdu edu cn We report the production and characterization of somatic hybrids between Triticum aestivum L and Agropyron elongatum Host Nevishi the synonym is Thinopyrum ponticum Asymmetric protoplast fusion was performed between Agropyron elongatum protoplasts irradiated with a low UV dose and protoplasts of wheat taken from nonregenerable suspension cultures More than 40 green plantlets were obtained from 15 regenerated clones and one of them produced seeds The phenotypes of the hybrid plants and seeds were intermediate between wheat and Agropyron elongatum All of the regenerated calli and plants were verified as intergeneric hybrids on the basis of morphological observation and analysis of isozyme cytological 5SrDNA spacer sequences and random amplified polymorphic DNA RAPD RFLP analysis of the mitochondrial genome revealed evidence of random segregation and recombination of mtDNA keywords Triticum aestivum Agropyron elongatum somatic hybridization

    Original URL path: http://www.cell-research.com/arts.asp?id=1285 (2016-02-14)
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  • Cell Research

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    Original URL path: /artsmore1.asp?id=108 (2016-02-14)


  • Cell Research
    Australia 2 Initiative for Biomolecular Structure School of Physics University of New South Wales NSW 2052 and Centre for Immunology St Vincent s Hospital and University of New South Wales Sydney NSW 2010 Australia Correspondence Cecily Oakley Tel 61 2 9351 3059 E mail cecily med usyd edu au The muscle protein myosin binding protein C MyBPC is a large multi domain protein whose role in the sarcomere is complex and not yet fully understood Mutations in MyBPC are strongly associated with the heart disease familial hypertrophic cardiomyopathy FHC and these experiments of nature have provided some insight into the intricate workings of this protein in the heart While some regions of the MyBPC molecule have been assigned a function in the regulation of muscle contraction the interaction of other regions with various parts of the myosin molecule and the sarcomeric proteins actin and titin remain obscure In addition several intra domain interactions between adjacent MyBPC molecules have been identified Although the basic structure of the molecule a series of immunoglobulin and fibronectin domains has been elucidated the assembly of MyBPC in the sarcomere is a topic for debate By analysing the MyBPC sequence with respect to FHC causing mutations

    Original URL path: http://www.cell-research.com/arts.asp?id=1264 (2016-02-14)
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