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  • Cell Research
    ISSUE 2 4 2004 111 116 Peptide nucleic acid PNA binding mediated gene regulation Gan WANG Xiaoxin S XU Institute of Environmental Health Sciences Wayne State University 2727 Second Avenue Detroit MI 48201 USA Correspondence Gan WANG Tel 313 964 8140 E mail wang wayne edu Peptide nucleic acids PNAs are synthetic oligonucleotides with chemically modified backbones PNAs can bind to both DNA and RNA targets in a sequence specific manner to form PNA DNA and PNA RNA duplex structures When bound to double stranded DNA dsDNA targets the PNA molecule replaces one DNA strand in the duplex by strand invasion to form a PNA DNA PNA or PNA 2 DNA triplex structure and the displaced DNA strand exists as a single stranded D loop PNA has been used in many studies as research tools for gene regulation and gene targeting The D loops generated from the PNA binding have also been demonstrated for its potential in initiating transcription and inducing gene expression PNA provides a powerful tool to study the mechanism of transcription and an innovative strategy to regulate target gene expression An understanding of the PNA mediated gene regulation will have important clinical implications in treatment of many

    Original URL path: http://www.cell-research.com/arts.asp?id=1265 (2016-02-14)
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  • Cell Research
    Department of Internal Medicine the University of Michigan Ann Arbor MI 48109 0934 USA Correspondence Man Chao ZHANG Tel 734 647 7949 E mail manchaoz umich edu LIGHT homologous to lymphotoxins shows inducible expression and competes with herpes simplex virus glycoprotein D for herpes virus entry mediator HVEM TR2 is a new member of TNF superfamily The HT 29 colon cancer cell line is the most sensitive to LIGHT induced IFNg mediated apoptosis among the cell lines we have examined so far Besides downregulation of Bcl XL upregulation of Bak and activation of both PARP poly ADP ribose polymerase and DFF45 DNA fragmentation factor LIGHT induced IFNg mediated apoptosis of HT 29 cells involves extensive caspase activation Caspase 8 and caspase 9 activation as shown by their cleavages appeared as early as 24 h after treatment whereas caspase 3 and caspase 7 activation as shown by their cleavages occurred after 72 h of LIGHT treatment Caspase 3 inhibitor Z DEVD FMK benzyloxycarbonyl Asp Glu Val Asp fluoromethylketone and a broad range caspase inhibitor Z VAD FMK benzyloxycarbonyl Val Ala Asp fluoromethylketone were able to block LIGHT induced IFNg mediated apoptosis of HT 29 cells The activity of caspase 3 which

    Original URL path: http://www.cell-research.com/arts.asp?id=1266 (2016-02-14)
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  • Cell Research
    Department of Immunology Peking University Health Science Center 38 Xueyuan Road Beijing 100083 China 2 The Walter and Eliza Hall Institute PO Royal Melbourne Hospital Victoria 3050 Australia Correspondence Wei Feng CHEN Tel 0086 10 82802593 E mail wfchen public bta net cn Mouse thymic stromal cell line 4 MTSC4 is one of the stromal cell lines established in our laboratory While losing the characteristics of epithelial cells they express some surface markers shared with thymic dendritic cells TDCs To further study the biological functions of these cells we compared the capability of MTSC4 with TDCs in the induction of thymocyte apoptosis using thymic reaggregation culture system Apoptosis of thymocytes induced by MTSC4 and TDCs was measured by Annexin V and PI staining and analyzed by flow cytometry We found that MTSC4 selectively augmented the apoptosis of CD4 8 DP thymocytes This effect was Fas FasL independent and could not be blocked by antibodies to MHC class I and class II molecules In addition MTSC4 enhanced the apoptosis of DP thymocytes from different strains of mice which implies that MTSC4 induced thymocyte apoptosis is not mediated by the TCR recognition of self peptide MHC molecules In contrast to MTSC4 thymocyte

    Original URL path: http://www.cell-research.com/arts.asp?id=1267 (2016-02-14)
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  • Cell Research
    in Xenopus egg extracts Zhi Gang LU Chuan Mao ZHANG Zhong He ZHAI Department of Cell Biology and Genetics College of Life Sciences Peking University Beijing 100871 China Correspondence Chuan Mao ZHANG Zhong He ZHAI Tel 86 10 62757173 86 10 62757173 E mail zhangcm pku edu cn zhonghezhai pku edu cn DNA degradation is a biochemical hallmark in apoptosis It has been demonstrated in many cell types that there are two stages of DNA fragmentation during the apoptotic execution In the early stage chromatin DNA is cut into large molecular weight DNA fragments although the responsible nuclease s has not been recognized In the late stage the chromatin DNA is cleaved further into short oligonucleosomal fragments by a well characterized nuclease in apoptosis the caspase activated DNase CAD DFF40 In this study we demonstrate that large molecular weight DNA fragmentation also occurs in Xenopus egg extracts in apoptosis We show that the large molecular weight DNA fragmentation factor LDFF is not the Xenopus CAD homolog XCAD LDFF is activated by caspase 3 The large molecular weight DNA fragmentation activity of LDFF is Mg 2 dependent and Ca 2 independent can occur in both acidic and neutral pH conditions and

    Original URL path: http://www.cell-research.com/arts.asp?id=1268 (2016-02-14)
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  • Cell Research
    University Shijiazhuang China Correspondence Wei AN Tel 0086 10 63291921 E mail anwei cpums edu cn Signal transducer and activator of transcription 3 STAT3 is a recently characterized transcription factor which is essential to liver regeneration We have previously reported that hepatic stimulator substance HSS a novel growth promoting substance phosphorylated the epidermal growth factor EGF receptors and activated downstream Ras MAP kinase extracellular signal regulated kinases ERK1 2 cascade However whether HSS signal is related to STAT3 pathway remains unclear The present study is aiming to explore the regulatory effect of activation of ERK1 2 evoked by HSS on STAT3 phosphorylation and STAT3 signaling Human hepatoma cell line HepG2 was stably transfected with HSS cDNA and HSS ex pression was measured by Northern blot The results showed that the transfection of HSS into HepG2 resulted in remarkable increase in cellular proliferation as compared with the non transfected cells and it was further proved that the cellular proliferation in the HSS transfected cells was related to ERK1 2 activation Treatment of the cells with 50 μM of PD98059 an ERK1 2 specific upstream inhibitor resulted in ERK1 2 inactivation completely Inhibition of ERK1 2 allowed the tyrosine of STAT3 to

    Original URL path: http://www.cell-research.com/arts.asp?id=1269 (2016-02-14)
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  • Cell Research
    Pharmaceutical Science Sun Yat Sen University Guangzhou 510089 China 3 Department of Immunology Nagoya University Graduate and Faculty School of Medicine Aichi 466 8550 Japan 4 College of Pharmacy Jinan University Guangzhou 510632 China Correspondence Jun DU Tel 81 52 719 1547 E mail togun met nagoya u ac jp H Ras is well known as one of the essential components of Ras Raf MEK ERK cascade which is a critical prosurvival signaling mechanism in most eukaryotic cells Ras targets Raf MEK ERK cascade by integrating and transmitting extracellular signals from growth factor receptors to Raf leading to the propagation of signals to modulate a serious of cellular survival events Apoptosis signal regulating kinase1 ASK1 serves as a general mediator of cell death because it is responsive to a variety of death signals In this study we found that H Ras interacted with ASK1 to cause the inhibition of both ASK1 activity and ASK1 induced apoptosis in vivo which was reversed only partially by addition of RafS621A an antagonist of Raf whereas MEK inhibitor PD98059 and PI3K inhibitor LY294002 did not disturb the inhibitory effect of H Ras on ASK 1 induced apoptosis Furthermore by means of immunoprecipitate and kinase

    Original URL path: http://www.cell-research.com/arts.asp?id=1270 (2016-02-14)
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  • Cell Research
    1 Mei Fu FENG 1 Rou Li ZHOU 2 1 Staate Key Laboratory of Biomembrane and Membrane Biotechnology Institute of Zoology Chinese Academy of Science Beijing 100080 China 2 Department of Cell Biology and Medical Genetics School of Basic Medical Sciences Health Science Center Peking University Beijing 100083 China Correspondence Mei Fu FENG Rou Li ZHOU Tel 86 10 62571017 408 731 5069 E mail fengmf panda ioz ac cn rlzhou bjmu edu cn The soluble HLA G1 sHLA G1 isoform was found to be secreted by trophoblast cells at the materno fetal interface which suggests that it may act as an immunomodulator during pregnancy In this paper we reported that GST sHLA G1α chain could bind to its receptor ILT 2 on NK92 cells and then the latter recruited Src homology 2 domain containing tyrosine phosphatase 1 SHP 1 which consequently dephosphorylated some important protein tyrosine kinases and blocked the activation of downstream molecules such as MEK and ERK so that the cytotoxicity of natural killer NK cells was inhibited These results indicated that GST sHLA G1α chain might be exploited in new immunotherapy strategies aiming at inducing immunotolerance during allograft xenograft and autoimmune situations In addition we found

    Original URL path: http://www.cell-research.com/arts.asp?id=1271 (2016-02-14)
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  • Cell Research
    Shu CHIEN5 1 Hemorheology Center Department of Biophysics School of Basic Medical Sciences Peking University Beijing 100083 China 2 Department of Biochemical and Molecular Biology School of Basic Medical Sciences Peking University Beijing 100083 China 3 Department of Urology Medical Center New York University USA 4 Department of Mechanics and Engineering Science Peking University Beijing 100871 China 5 Department of Bioengineering and Whitaker Institute of Biomedical Engineering University of California San Diego La Jolla CA 92093 0412 USA Correspondence Zong Yao WEN Tel 0086 10 62092419 E mail rheol mail bjmu edu cn The cDNA fragment of human TRAIL TNF related apoptosis inducing ligand was cloned into RevTet On a Tet regulated and high level gene expression system The gene expression system was constructed in a human leukemic cell line Jurkat By using RevTet On TRAIL gene expression system in Jurkat as a cell model we studied the influence of TRAIL gene on the changes of cellular apoptosis before and after the TRAIL gene expression which was induced by adding tetracycline derivative doxycycline Dox The results indicated that the cellular apoptosis ratio was largely dependent on the trail gene expression level Moreover it was found that the apoptosis inducing TRAIL

    Original URL path: http://www.cell-research.com/arts.asp?id=1272 (2016-02-14)
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