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  • Cell Research
    Bo HU 1 3 1 College of Life Science Shenzhen Key Laboratory of Microbial Gene Engineering Shenzhen University Shenzhen 518060 China 2 Key Laboratory for Molecular Enzymology and Engineering of Ministry of Education Jilin University Changchun 130021 China 3 Institute of Genetics and Cytology Northeast Normal University Changchun 130024 China Correspondence Miao XING E mail xingmiao szu edu cn CDKs play key roles in controlling cell cycle progression in all eukaryotes In plants multiple CDKs are present among which the best characterized CDKs are PSTAIRE CDKs In this study we carried out Western blot immunoelectron microscopy and antibody treatment with an anti PSTAIRE monoclonal antibody to explore the subcellular localization and functions of PSTAIRE CDKs in Physarum polycephalum The results of western blot and immunoelectron microscopy showed that in P polycephalum a PSTAIRE CDK like protein was 34 kD in molecular weight and located in both nuclei and cytoplasm In nuclei the protein was mainly associated with chromosomes and nucleoli The expression of the PSTAIRE CDK like protein in both the plasmodia and nuclei showed little fluctuation through the whole cell cycle When treated with an anti PSTAIRE monoclonal antibody at early S phase the cells were arrested in S

    Original URL path: http://www.cell-research.com/arts.asp?id=1273 (2016-02-14)
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  • Cell Research

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    Original URL path: /artsmore1.asp?id=107 (2016-02-14)


  • Cell Research
    6 2004 179 187 Answering a century old riddle brachydactyly type A1 Bo Gao 2 3 Lin He 1 2 1 Institute for Nutritional Sciences Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai 200031 China 2 Neuropsychiatric and Human Genetics Group Bio X Life Science Research Center Shanghai Jiaotong University Shanghai 200030 China 3 Department of Biochemistry Faculty of Medicine The University of Hong Kong Hong Kong China Correspondence Lin HE Tel 86 21 64717740 E mail helin nhgg org In 1903 Farabee analyzed the heredity of the human digital malformation brachydactyly the first recorded disorder of the autosomal dominant Mendelian trait In 1951 Bell classified this type of brachydactyly as type A1 BDA1 Over 100 cases from different ethnic groups have so far been reported However the real breakthrough in identifying the cause of BDA1 has only taken place in the last few years with the progress of the mapping and identification of one of the genes responsible for this disorder thus providing an answer for a century old riddle In this article we attempt to review the current state of knowledge on the genetic features of BDA1 with its century old history and signalling pathway of

    Original URL path: http://www.cell-research.com/arts.asp?id=1255 (2016-02-14)
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  • Cell Research
    effect by diosgenin through activation of p53 release of apoptosis inducing factor AIF and modulation of caspase 3 activity in different human cancer cells Cecile CoRBIERE 1 Bertrand LIAGRE 1 Faraj TERRO 2 Jean Louis BENEYTOUT 1 1 Laboratoire de Biochimie UPRES EA 1085 Faculté de Pharmacie 2 rue du Docteur Marcland 87025 Limoges Cedex France 2 Service d Histologie et Biologie Cellulaire Faculté de Médecine 2 rue du Docteur Marcland 87025 Limoges Cedex France Correspondence Bertrand Liagre Tel 33 555 43 58 39 E mail bertrand liagre unilim fr Previously we demonstrated that a plant steroid diosgenin altered cell cycle distribution and induced apoptosis in the human osteosarcoma 1547 cell line The objective of this study was to investigate if the antiproliferative effect of diosgenin was similar for different human cancer cell lines such as laryngocarcinoma HEp 2 and melanoma M4Beu cells Moreover this work essentially focused on the mitochondrial pathway We found that diosgenin had an important and similar antiproliferative effect on different types of cancer cells In addition our new results show that diosgenin induced apoptosis is caspase 3 dependent with a fall of mitochondrial membrane potential nuclear localization of AIF and poly ADP ribose polymerase cleavage

    Original URL path: http://www.cell-research.com/arts.asp?id=1256 (2016-02-14)
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  • Cell Research
    Jian Chao ZHANG 3 Shu Dong ZONG 3 Shi Ying MIAO 1 Lin Fang WANG 1 Samuel S KOIDE 2 1 National Laboratory of Medical Molecular Biology Institute of Basic Medical Sciences Chinese Academy of Medical Sciences Peking Union Medical College 5 Dong Dan San Tiao Beijing 100005 China 2 Center for Biomedical Research Population Council 1230 York Avenue New York NY 10021 USA 3 National Research Institute for Family Planning 12 Da Hui Si Hai Dian Beijing 100081 China Correspondence Lin Fang WANG Tel 86 10 6524 0803 E mail wanglf ms imicams ac cn A cDNA designated as rtSH3p13 was isolated from a rat testis cDNA library It consists of 1463 bp nuclear acids which encodes a protein of 312 amino acids and was assigned the GenBank accession number AF227439 The deduced rtSH3p13 protein is a truncated isoform of SH3p13 as a result of mRNA alternative splicing It is mainly expressed in the rat testis detected in spermatids at the steps 8 19 of spermiogenesis and found around the acrosome During postnatal development rtSH3p13 appears on day 18 and reaches maximum on day 60 Further experimental results suggested that rtSH3p13 forms a complex with activated epidermal growth factor

    Original URL path: http://www.cell-research.com/arts.asp?id=1257 (2016-02-14)
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  • Cell Research
    Chun Fu WU 1 Guo Ping ZHAO 2 4 1 Pharmaceutical Department Shenyang Pharmaceutical University 103 Wenhua Road Shenhe District Shenyang 110016 China 2 Research Center of Biotechnology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences 500 Caobao Road Shanghai 200233 China 3 Department of Microbiology and Parasitology Shanghai Second Medical University 280 Chongqingnan Road Shanghai 200025 China 4 Chinese National Human Genome Center at Shanghai CHGCS 250 Bibo Road ZhangJiang High Tech Park Shanghai 201203 China Correspondence Guo Ping ZHAO Tel 86 21 50801919 E mail gpzhao sibs ac cn Comparative genomic analysis of the coding sequences CDSs of Leptospira interrogans revealed a pair of closely linked genes homologous to the vapBC loci of many other bacteria with respect to both deduced amino acid sequences and operon organizations Expression of single vapC gene in Escherichia coli resulted in inhibition of bacterial growth whereas co expression of vapBC restored the growth effectively This phenotype is typical for three other characterized toxin antitoxin systems of bacteria i e mazEF 1 relBE 2 and chpIK 3 The VapC proteins of bacteria and a thermophilic archeae Solfolobus tokodaii form a structurally distinguished group of toxin different from the other known toxins of

    Original URL path: http://www.cell-research.com/arts.asp?id=1258 (2016-02-14)
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  • Cell Research
    of DNA class SpellE methylation and histone class SpellE acetylation alone or in combination on the expression of several tumor associated genes and cell cycle progression in two established human colon cancer cell lines Colo 320 and SW1116 Treatments with 5 aza UnitName SourceValue 2 HasSpace False Negative True NumberType 1 TCSC 0 w st on 2 deoxycytidine 5 aza dC and class SpellE trichostatin A alone or in combination were applied respectively The methylation status of the CDKN UnitName a SourceValue 2 HasSpace False Negative False NumberType 1 TCSC 0 w st on 2A promoter was determined by class SpellE methylation specific PCR and the acetylated status of the class SpellE histones associated with the p21 WAF1 and CDKN UnitName a SourceValue 2 HasSpace False Negative False NumberType 1 TCSC 0 w st on 2A genes was examined by chromatin class SpellE immunoprecipitation The expression of the CDKN UnitName a SourceValue 2 HasSpace False Negative False NumberType 1 TCSC 0 w st on 2A p21 WAF1 p53 p73 APC c myc c class SpellE Ki ras and survivin genes was detected by real time RT PCR and RT PCR The cell cycle profile was established by flow cytometry color windowtext We found that along with the demethylation of the CDKN Negative False NumberType 1 TCSC 0 w st on 2A gene promoter in both cell lines induced by 5 aza dC alone or in combination with TSA the expression of both CDKN HasSpace False Negative False NumberType 1 TCSC 0 w st on 2A and APC genes increased The treatment of TSA or sodium butyrate up regulated the transcription of p21 WAF1 significantly by inducing the acetylation of histones H4 and H3 but failed to alter the acetylation level of CDKN Negative False NumberType 1 TCSC 0 w st

    Original URL path: http://www.cell-research.com/arts.asp?id=1259 (2016-02-14)
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  • Cell Research
    Biological Sciences Chinese Academy of Sciences 320 Yueyang Road Shanghai 200031 China Correspondence Jia Rui WU Tel 86 21 54921128 E mail wujr sibs ac cn DNA replication is tightly regulated during the S phase of the cell cycle and the activation of the intra S phase checkpoint due to DNA damage usually results in arrest of DNA synthesis However the molecular details about the correlation between the checkpoint and regulation of DNA replication are still unclear To investigate the connections between DNA replication and DNA damage checkpoint a DNA damage reagent tripchlorolide was applied to CHO Chinese ovary hamster cells at early or middle stages of the S phase The early S phase treatment with TC significantly delayed the progression of the S phase and caused the phosphorylation of the Chk1 checkpoint protein whereas the middle S phase treatment only slightly slowed down the progression of the S phase Furthermore the analysis of DNA replication patterns revealed that replication pattern II was greatly prolonged in the cells treated with the drug during the early S phase whereas the late replication patterns of these cells were hardly detected suggesting that the activation of the intra S phase checkpoint inhibits the

    Original URL path: http://www.cell-research.com/arts.asp?id=1260 (2016-02-14)
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