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  • Cell Research
    6 Kashima Yodogawa ku Osaka 532 5814 Japan Correspondence Paul Alexander Jones Tel 44 0 131 6511897 E mail P Jones ed ac uk Characterising the mechanisms of cell death following focal cerebral ischaemia has been hampered by a lack of an in vitro assay emulating both the apoptotic and necrotic features observed in vivo The present study systematically characterised oxygen glucose deprivation OGD in primary rat cortical neurones to establish a reproducible model with components of both cell death endpoints OGD induced a time dependent reduction in cell viability with 80 cell death occurring 24 h after 3 h exposure to 0 O 2 and 0 5 mM glucose Indicative of a necrotic component to OGD induced cell death N methyl D aspartate NMDA receptor inhibition with MK 801 attenuated neuronal loss by 60 The lack of protection by the caspase inhibitors DEVD CHO and z VAD fmk suggested that under these conditions neurones did not die by an apoptotic mechanism Moderating the severity of the insult by decreasing OGD exposure to 60 min did not reduce the amount of necrosis but did induce a small degree of apoptosis a slight reduction in cell death was observed in the

    Original URL path: http://www.cell-research.com/arts.asp?id=1262 (2016-02-14)
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  • Cell Research
    Technology and Medicine The Chelsea and Westminster Hospital 369 Fulham Road London SW10 9NH UK Correspondence Justin STEBBING Tel 011 44 208 746 8251 E mail j stebbing imperial ac uk There is increasing recognition of the potential morbidity and mortality associated with HIV 1 and hepatitis C HCV co infection HIV appears to adversely affect HCV disease while the reciprocal effect of HCV on HIV remains controversial We therefore studied the effect of co infection on dendritic cell function versus HIV infection alone as previous work has shown that HCV impairs dendritic cell DC function HIV 1 positive individuals with HCV were matched for CD4 count HIV 1 RNA viral load and therapy to HIV 1 positive patients without HCV Monocyte derived DC were generated and mixed leukocyte reactions were performed We assessed allostimulatory capacity with and without administration of exogenous Th1 cytokines using thymidine uptake and cell division analyses with the vital dye CFSE We found that monocyte derived DC from co infected individuals showed no significant differences in allostimulatory capacity to ex vivo generated DC from HIV 1 infected individuals without HCV Unlike the situation with HCV infection alone this impairment was not reversed by increasing concentrations

    Original URL path: http://www.cell-research.com/arts.asp?id=1263 (2016-02-14)
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  • Cell Research

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    Original URL path: /artsmore1.asp?id=106 (2016-02-14)


  • Cell Research
    brief commentary on the diversity of placentation mechanisms and then goes on to examine the extensive alterations which occur in the plasma membrane of uterine epithelial cells during early pregnancy across species Ultrastructural biochemical and more general morphological data reveal that strikingly common phenomena occur in this plasma membrane during early pregnancy despite the diversity of placental types from epitheliochorial to hemochorial which ultimately form in different species To encapsulate

    Original URL path: http://www.cell-research.com/arts.asp?id=1247 (2016-02-14)
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  • Cell Research
    The John P Robarts Research Institute 100 Perth Drive London Ontario N6A 5K8 Canada 2 Department of Microbiology and Immunology The University of Western Ontario London Ontario Canada 3 Department of Physiology The University of Western Ontario London Ontario Canada Correspondence Mickie BHATIA Tel 519 663 5777 Ext 34166 E mail mbhatia robarts ca With the exception of mature erythrocytes cells within the human hematopoietic system are characterized by the cell surface expression of the pan leukocyte receptor CD45 Here we identify a novel subset among mononuclear cord blood cells depleted of lineage commitment markers Lin that are devoid of CD45 expression Surprisingly functional examination of Lin CD45 cells also lacking cell surface CD34 revealed they were capable of multipotential hematopoietic progenitor capacity Co culture with mouse embryonic limb bud cells demonstrated that Lin CD45 CD34 cells were capable of contributing to cartilage nodules and differentiating into human chondrocytes BMP 4 a mesodermal factor known to promote chondrogenesis significantly augmented Lin CD45 CD34 differentiation into class SpellE chondrocytes Moreover unlike CD34 human class SpellE hematopoietic stem cells Lin CD45 CD34 cells were unable to proliferate or survive in liquid cultures whereas single Lin CD45 CD34 cells were able to class

    Original URL path: http://www.cell-research.com/arts.asp?id=1248 (2016-02-14)
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  • Cell Research
    transcription silencing of the MAGE A1 gene Jie ZHANG Jian YU Jun GU Bao Mei GAO Ying Jun ZHAO Peng WANG Hong Yu ZHANG Jing De ZHU The State key Laboratory for Oncogenes and Related Genes Shanghai Cancer Institute Shanghai Jiao Tong University LN 2200 25 Xietu Road Shanghai 200032 China Correspondence Jing De ZHU Tel 86 21 64224285 E mail zhujingde yahoo com zhujingde 21cn com To understand the DNA methylation mediated gene silencing mechanisms we analyzed in cell culture of the promoter function of the MAGE A1 gene which is frequently demethylated and over expressed in human hepatocellular carcinoma We have established the correlation of the DNA methylation of the promoter CpG island with expression status of this gene in a panel of the established liver cancer cell lines The crucial CpG dinucleotide s within the minimal promoter subjected to the control mediated by DNA methylation with profound biological functions was also delineated Furthermore a novel sequence specific DNA protein interaction at the 30 CpG dinucleotide upstream of the gene was found having a vital part to play in the DNA methylation mediated transcription silencing of the MAGE A1 gene Our results would not only provide new insights

    Original URL path: http://www.cell-research.com/arts.asp?id=1249 (2016-02-14)
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  • Cell Research
    University Busan Korea 3 Virus Tumor Biology Section Laboratory of Cellular Oncology Center for Cancer Research National Cancer Institutes National Institute of Health Bethesda MD 20892 USA Correspondence Soo Jin JEONG Tel 01 301 496 0986 E mail jeongs mail nih gov Ionizing radiation is one of the most effective tools in cancer therapy In a previous study we reported that protein tyrosine kinase PTK inhibitors modulate the radiation responses in the human chronic myelogenous leukemia CML cell line K562 The receptor tyrosine kinase inhibitor genistein delayed radiation induced cell death while non recepter tyrosine kinase inhibitor herbimycin A HMA enhances radiation induced apoptosis In this study we focused on the modulation of radiation induced cell death by genistein and performed PCR select suppression subtractive hybridization SSH to understand its molecular mechanism We identified human thymidine kinase 1 TK1 which is cell cycle regulatory gene and confirmed expression of TK1 mRNA by Northern blot analysis Expression of TK1 mRNA and TK 1 enzymatic activity were parallel in their increase and decrease TK1 is involved in G1 S phase transition of cell cycle progression In cell cycle analysis we showed that radiation induced G2 arrest in K562 cells but it was

    Original URL path: http://www.cell-research.com/arts.asp?id=1250 (2016-02-14)
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  • Cell Research
    LEI 1 Zhen Zhen WANG 1 Catherine CY CHANG XinYing YANG 1 Ta Yuan CHANG 2 Bo Liang LI 1 1 State Key Laboratory of Molecular Biology Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Graduate School of the Chinese Academy of Sciences 320 Yueyang Road Shanghai 200031 China 2 Department of Biochemistry Dartmouth Medical School Hanover NH 03756 USA Correspondence Ta Yuan CHANG Bo Liang LI Tel 603 650 1622 86 21 5492 1278 E mail Ta Yuan Chang dartmouth edu blli sibs ac cn In macrophages the accumulation of cholesteryl esters synthesized by the activated acyl coenzyme A cholesterol acyltransferase 1 ACAT1 results in the foam cell formation a hallmark of early atherosclerotic lesions In this study with the treatment of a glucocorticoid hormone dexamethasone Dex lipid staining results clearly showed the large accumulation of lipid droplets containing cholesteryl esters in THP 1 derived macrophages exposed to lower concentration of the oxidized low density lipoprotein ox LDL More notably when treated together with specific anti ACAT inhibitors the abundant cholesteryl ester accumulation was markedly diminished in THP 1 derived macrophages confirming that ACAT is the key enzyme responsible for intracellular cholesteryl ester synthesis RT PCR and Western blot results indicated that Dex caused up regulation of human ACAT1 expression at both the mRNA and protein levels in THP 1 and THP 1 derived macrophages The luciferase activity assay demonstrated that Dex could enhance the activity of human ACAT1 gene P1 promoter a major factor leading to the ACAT1 activation in a cell specific manner Further experimental evidences showed that a glucocorticoid response element GRE located within human ACAT1 gene P1 promoter to response to the elevation of human ACAT1 gene expression by Dex could be functionally bound with glucocorticoid receptor GR

    Original URL path: http://www.cell-research.com/arts.asp?id=1251 (2016-02-14)
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