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  • Cell Research
    Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Graduate School of the Chinese Academy of Sciences 320 Yueyang Rd Shanghai 200031 2 College of Life Science Jilin University Changchun 130061 China 3 Department of Genetics and Developmental Biology University of Connecticut Health Center Farmington Connecticut 06030 USA Correspondence Lin Li Tel 86 21 5492 1188 E mail lli sibs ac cn Wnt signaling plays an important role in embryogenesis and tumorgenesis Although the mechanism about how Wnts transduce their signaling from receptor frizzled Fz to cytosol has not been understood dishevelled Dvl protein was considered as the intersection of Wnt signal traffic In this study we characterized the function of three domains DIX PDZ and DEP of Dvl 1 in canonical Wnt signal transduction and Dvl 1 membrane translocation It was found both DIX and DEP domain were sufficient to block Wnt 3a induced LEF 1 transcriptional activity and free cytosol β catenin accumulation whereas PDZ domain and a functional mutant form of DEP domain DEP KM had no effect on canonical Wnt signaling In addition when cotransfected with Fz 7 DEP domain but not DIX PDZ or DEP KM translocated and co

    Original URL path: http://www.cell-research.com/arts.asp?id=1252 (2016-02-14)
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  • Cell Research
    S 205 02 Malmo Sweden 2 Laboratory of Nerve Cell Biophysics Institute of Cell Biophysics Russian Academy of Sciences Pushchino Moscow region 142290 Russia Correspondence Anders BREDBERG Tel 46 40 337414 E mail anders bredberg mikrobiol mas lu se Human polymorphonuclear leukocytes PMN have been reported to completely lack of DNA dependent protein kinase DNA PK which is composed of Ku protein and the catalytic subunit DNA PKcs needed for nonhomologous end joining NHEJ of DNA double strand breaks Promyelocytic HL 60 cells express a variant form of Ku resulting in enhanced radiation sensitivity This raises the question if low efficiency of NHEJ instrumental for the cellular repair of oxidative damage is a normal characteristic of myeloid differentiation Here we confirmed the complete lack of DNA PK in PMN protein extracts and the expression of the truncated Ku86 variant form in HL 60 However this degradation of DNA PK was shown to be due to a DNA PK degrading protease in PMN and HL 60 In addition by using a protease resistant whole cell assay both Ku86 and DNA PKcs could be demonstrated in PMN suggesting the previously reported absence in PMN of DNA PK to be an artefact The

    Original URL path: http://www.cell-research.com/arts.asp?id=1253 (2016-02-14)
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  • Cell Research
    Animal Reproduction and Embryology College of Animal Sciences and Veterinary Medicine Shandong Agricultural University Taian Shandong 271018 China Correspondence Jing He TAN Tel 86 0538 8249616 E mail tanjh sdau edu cn It is well known that during mammalian ovarian follicular development the majority of follicles undergo atresia at various stages of their development However the mechanisms controlling this selection process remain unknown In this study we investigated apoptosis in granulosa cells during goat follicular atresia by terminal deoxynucleotidyl transferase mediated dUTP nick end labeling TUNEL The changes in the levels of steroids insulin like growth factors IGFs and IGF receptors were studied by radioimmunoassay RIA and semi quantitative reverse transcription PCR We found that the percentage of apoptotic granulosa cells in the atretic A follicles was significantly higher than that in the slightly atretic SA and healthy H follicles The level of estradiol and the ratio of estradiol to progesterone in H follicles were significantly higher than those in A follicles On the other hand the level of progesterone was not significantly different among these follicle types We also found that the level of IGF I in H follicles was higher than in SA and A follicles whereas the

    Original URL path: http://www.cell-research.com/arts.asp?id=1254 (2016-02-14)
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  • Cell Research

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    Original URL path: /artsmore1.asp?id=105 (2016-02-14)


  • Cell Research
    2 Health Science Center Shanghai Institutes for Biological Sciences Shanghai 2nd Medical University Shanghai China 3 Institutes of Biomedical Sciences Ruijin Hospital Shanghai 2nd Medical University 197 Ruijin Er Road Shanghai China 4 Model Organism Division E Institutes of Shanghai Universities Shanghai China Correspondence Wei Dong LE Tel 86 21 64370045 ext 611209 E mail wdl sibs ac cn Biomarkers are very important indicators of normal and abnormal biological processes Specific changes in pathologies biochemistries and genetics can give us comprehensive information regarding the nature of any particular disease A good biomarker should be precise and reliable distinguishable between normal and interested disease and differential between different diseases It is believed that biomarkers have great potential in predicting chances for diseases aiding in early diagnosis and setting standards for the development of new remedies to treat diseases New technologies have enabled scientists to identify biomarkers of several different neurodegenerative diseases The followings for instance are only a few of the many new biomarkers that have been recently identified the phosphorylated tau protein and aggregated β amyloid peptide for Alzheimer s disease AD α synuclein contained Lewy bodies and altered dopamine transporter DAT imaging for Parkinson s disease PD SOD mutations

    Original URL path: http://www.cell-research.com/arts.asp?id=1236 (2016-02-14)
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  • Cell Research
    John Radcliffe Hospital Oxford OX3 9DS UK 2 Department of Medicine Hammersmith Hospital Imperial College Du Cane Road London W12 0NN UK Correspondence Gavin R Screaton E mail g screaton imperial ac uk Identification of tumour necrosis factor apoptosis inducing ligand TRAIL a TNF family ligand sparked a torrent of research following an initial observation that it could kill tumour cells but spare normal cells Almost a decade after its

    Original URL path: http://www.cell-research.com/arts.asp?id=1237 (2016-02-14)
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  • Cell Research
    Nosek 2 1 Department of Physiology and Biophysics UMDNJ Robert Wood Johnson Medical School Piscataway NJ 08854 USA 2 Department of Physiology and Biophysics School of Medicine Case Western Reserve University Cleveland OH 44106 USA 3 Department of Biochemistry Graduate School of Medicine Tohoku University 2 1 Seiryo machi Aoba ku Sendai Miyagi 980 8575 Japan Correspondence Marco A P Brotto E mail brottoma umdnj edu Mitsugumin 29 MG29 is a transmembrane protein that is normally found in the triad junction of skeletal muscle Our previous studies have shown that targeted deletion of mg29 from the skeletal muscle resulted in abnormality of the triad junction structure and also increased susceptibility to muscle fatigue To elucidate the basis of these effects we investigated the properties of Ca 2 uptake and release in toxin skinned Extensor Digitorium Longus EDL muscle fibers from control and mg29 knockout mice Compared with the control muscle submaximal Ca 2 uptake into the sarcoplasmic reticulum SR was slower and the storage of Ca 2 inside the SR was less in the mutant muscle due to increased leakage process of Ca 2 movement across the SR The leakage pathway is associated with the increased sensitivity of Ca 2

    Original URL path: http://www.cell-research.com/arts.asp?id=1238 (2016-02-14)
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  • Cell Research
    Center Kansas City MO 64128 USA Correspondence Lisa Stehno Bittel Tel 01 913 588 4570 E mail lbittel kumc edu Spontaneous Ca 2 oscillations in vascular smooth muscle cells have been modeled using a single Ca 2 pool This report describes spontaneous Ca 2 oscillations dependent on two separate Ca 2 sources for the nuclear versus cytoplasmic compartments Changes in free intracellular Ca 2 were monitored with ratiometric Ca 2 fluorophores using confocal microscopy On average spontaneous oscillations developed in 79 of rat aortic smooth muscle cells that were synchronous between the cytoplasm and nucleus Reduction of extracellular Ca 2 and amplitude of the cytoplasmic oscillations with 48 of the oscillations asynchronous between the nuclear and cytoplasmic compartments Similar results were obtained with the Ca 2 channel blockers nimodipine and diltiazem Arg vasopressin AVP induced a rapid release of intracellular Ca 2 stores that was greater in the nuclear compartment 4 20 0 23 ratio units n 56 than cytoplasm 2 54 0 28 in cells that had spontaneously developed prior oscillations Conversely cells in the same conditions lacking oscillations had a greater AVP induced Ca 2 transient in the cytoplasm 4 99 0 66 n 17 than in the

    Original URL path: http://www.cell-research.com/arts.asp?id=1239 (2016-02-14)
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