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  • Cell Research
    Overexpression of the tissue inhibitor of metalloproteinase 3 during Xenopus embryogenesis affects head and axial tissue formation Bryce Pickard Sashko Damjanovski Department of Biology University of Western Ontario London Ontario Canada Correspondence Sashko Damjanovski Tel 519 661 2111 x 84704 E mail sdamjano uwo ca Tissue inhibitors of metalloproteinases TIMPs modulate extracellular matrix remodeling during embryonic development and disease TIMP 3 expression was examined during Xenopus laevis embryogenesis TIMP 3 transcripts detected in the maternal pool of RNA increased at the mid blastula transition decreased dramatically during gastrulation and increased again during neurulation and axis elongation Interestingly the decrease during gastrulation was not seen in LiCl treated dorsalized embryos Whole mount in situ hybridization of TIMP 3 using DIG labeled RNA probes demonstrated that the transcripts were present in all dorsal tissues during embryogenesis but were prominent only in head structures starting at stage 35 Overexpression of TIMP 3 through transgenesis and RNA injections led to developmental abnormalities and death Both overexpression strategies resulted in post gastrulation perturbation including those to neural and head structures as well as truncated axes However RNA injections resulted in more severe early defects such as failure of neural tube closure and transgenesis caused truncated

    Original URL path: http://www.cell-research.com/arts.asp?id=1240 (2016-02-14)
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  • Cell Research
    JIANG 2 Er Hei DAI 4 Xiao Yi WANG 4 Hua Liang JIANG 3 You Hua Xie 1 Xue Liang Zhu 1 Gang PEI 1 Lin LI 1 Jia Rui WU 1 Bing SUN 1 1 Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences 320 Yueyang Road Shanghai 200031 China 2 Institute of Microbiology and Epidemiology Institute of Basic Medical Sciences Academy of Military Medical Sciences Beijing 100071 China 3 Institute of Plant Physiology and Ecology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences 300 Fenglin Road Shanghai 200031 China 4 Institute of Materia Medica Shanghai Institutes for Biological Sciences Chinese Academy of Sciences 320 Taiyuan Road Shanghai 200031 China Correspondence Jia Rui WU Bing SUN Tel 0086 21 54921376 0086 21 54921376 E mail wujr sibs ac cn bsun sibs ac cn Spike protein is one of the major structural proteins of severe acute respiratory syndrome coronavirus It is essential for the interaction of the virons with host cell receptors and subsequent fusion of the viral envelop with host cell membrane to allow infection Some spike proteins of coronavirus such as MHV HCoV OC43 AIBV and BcoV are proteolytically cleaved into two subunits S1 and S2 In contrast TGV FIPV and HCoV 229E are not Many studies have shown that the cleavage of spike protein seriously affects its function In order to investigate the maturation and proteolytic processing of the S protein of SARS CoV we generated S1 and S2 subunit specific antibodies Abs as well as N E and 3CL protein specific Abs Our results showed that the antibodies could efficiently and specifically bind to their corresponding proteins from E coli expressed or lysate of SARS CoV infected Vero E6 cells by Western blot analysis Furthermore the anti S1 and

    Original URL path: http://www.cell-research.com/arts.asp?id=1241 (2016-02-14)
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  • Cell Research
    PEAc1 Ai Xiao LIU Shao Bin ZHANG Xiao Jing XU Dong Tao REN Guo Qin LIU State Key Laboratory of Plant Physiology and Biochemistry College of Biological Science China Agricultural University Beijing 100094 China Correspondence Guo Qin Liu Tel 86 10 62893438 E mail Liu cau edu cn A pea actin isoform PEAc1 with green fluorescent protein GFP fusion to its C terminus and His tag to its N terminus was expressed in prokaryotic cells in soluble form and highly purified with Ni Chelating SepharoseTM Fast Flow column The purified fusion protein PEAc1 GFP efficiently inhibited DNase I activities before polymerization and activated the myosin Mg ATPase activities after polymerization The PEAc1 GFP also polymerized into green fluorescent filamentous structures with a critical concentration of 0 75 µM These filamentous structures were labeled by TRITC phalloidin a specific agent for staining actin microfilaments and identified as having 9 nm diameters by negative staining These results indicated that PEAc1 preserved the essential characteristics of actin even with His tag and GFP fusion suggesting a promising potential to use GFP fusion protein in obtainning soluble plant actin isoform to analyze its physical and biochemical properties in vitro The PEAc1 GFP was also

    Original URL path: http://www.cell-research.com/arts.asp?id=1242 (2016-02-14)
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  • Cell Research
    Vasiliki KALFAKAKOU 1 1 Laboratory of Experimental Physiology University of Ioannina Medical School 45110 Ioannina Greece 2 Department of Anesthesia Pain Relief and Palliative Care University Clinic Areteion Hospital Athens Greece 3 Laboratory of Molecular and Cellular Aging Institute of Biological Research and Biotechnology National Hellenic Research Foundation 11635 Athens Greece Correspondence Evangelos Kolettas Vasiliki Kalfakakou Tel 30 26510 97578 97579 30 26510 97578 97579 E mail ekoletas cc uoi gr vkalfaka cc uoi gr Local anesthetics inhibit cell proliferation and induce apoptosis in various cell types Ropivacaine a unique novel tertiary amine type anesthetic was shown to inhibit the proliferation of several cell types including keratinocytes We found that Ropivacaine could inhibit the proliferation and induce apoptosis in an immortalized human keratinocyte line HaCaT in a dose and time dependent manner and with the deprivation of serum The dose dependent induction of apoptosis by ropivacaine was demonstrated by DNA fragmentation analysis and the proteolytic cleavage of a caspase 3 substrate poly ADP ribose polymerase PARP In addition ropivacaine downregulated the expression of clusterin apoliporotein J a protein with anti apoptotic properties in a dose dependent manner which well correlated with the induction of apoptosis of HaCaT cells To investigate the role of clusterin apoliporotein J in ropivacaine induced apoptosis HaCaT cells overexpressing clusterin apoliporotein J were generated and compared to cells expressing the well established anti apoptotic Bcl 2 protein Ectopic overexpression of the secreted form of clusterin apoliporotein J or Bcl 2 decreased the sensitivity of HaCaT cells to toxic effects of ropivacaine as demonstrated by DNA fragmentation the proteolytic cleavage of PARP and by a reduction in procaspase 3 expression Furthermore the downregulation of endogenous clusterin apolipoprotein J levels by ropivacaine suggested that this might be one mechanism by which ropivacaine induced cell death in HaCaT cells

    Original URL path: http://www.cell-research.com/arts.asp?id=1243 (2016-02-14)
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  • Cell Research
    University Shanghai 200032 China Correspondence Xing Zhong WU Tel 0086 21 54237697 E mail xz wu shmu edu cn It is well documented that the glycosylation of E cadherin is correlated with cancer metastasis but whether E cadherin could be core fucosylated remains largely unknown We found that E cadherin was core fucosylated in highly metastatic lung cancer cells while absent in lowly metastatic lung cancer cells Since α 1 6 Fucosyltransferase α 1 6 FucT is known to catalyze the reaction of core fucosylation we investigated the biological function of core fucosylation on E cadherin by α 1 6 FucT targeted RNAi and transfecting α 1 6 FucT expression vector As a result calcium dependent cell cell adhesion mediated by E cadherin was strengthened with the reduction of core fucosylation on E cadherin after RNAi and was weakened with the elevated core fucosylation on E cadherin after α 1 6 FucT over expression Our data indicated that α 1 6 FucT could regulate E cadherin mediated cell adhesion and thus play an important role in cancer development and progression Computer modeling showed that core fucosylation on E cadherin could significantly impair three dimensional conformation of N glycan on E

    Original URL path: http://www.cell-research.com/arts.asp?id=1244 (2016-02-14)
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  • Cell Research
    stability of p53 and cell proliferation Zhi Min Yin 1 Jian SiMa 1 Yi Fan Wu 1 Jian Zhu 1 Yong Jiang 2 1 Jiangsu Province Key Laboratory of Biochemistry and Molecular Biology College of Life Science Nanjing Normal University Nanjing 210097 China 2 Department of Pathophysiology and Key Lab for Shock and Microcirculation of PLA The First Military Medical University Guangzhou 510515 China Correspondence Zhi Min YIN Tel 86 25 86214554 E mail Zhiminy 2000 yahoo com The basal activity of JNK is low in normal growing cells and inactivated JNK targets p53 for ubiquitination To elucidate if the C terminal part of JNK is responsible for its binding to p53 the low background tet off inducible NIH3T3 cell line was selected by luciferase reporter gene and a double stable C JNK Aa 203 424 cell line was established After withdrawing tetracycline the C JNK fragment expression was induced and cell growth was dramatically inhibited 24 h later However the expresion of p53 was found to be increased after the induction of C JNK fragment evaluated by transfecting p21waf luciferase reporter genes Our further studies showed that C JNK fragment could form complex with p53 both in vivo and

    Original URL path: http://www.cell-research.com/arts.asp?id=1245 (2016-02-14)
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  • Cell Research
    10 VOLUME 14 ISSUE 5 10 2004 439 440 A new trick to tune down TGFβ signal Jian Zhang Center for Developmental Biology Institute of Genetics and Developmental Biology Chinese Academy of Sciences No 3 Nan Yi Tiao Zhong Guan Cun Beijing 100080 China Correspondence Jian Zhang E mail jianzhang84 genetics ac cn Signal transduction in early embryogenesis needs to be properly controlled A new player involved in tuning down

    Original URL path: http://www.cell-research.com/arts.asp?id=1246 (2016-02-14)
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  • Cell Research

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    Original URL path: /artsmore1.asp?id=104 (2016-02-14)




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