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  • Cell Research
    Silver Spring MD 20910 7500 USA 2 Departments of Medicine and of Pharmacology Uniformed Services University of The Health Sciences Bethesda MD 20814 4799 USA Correspondence Juliann G Kiang E mail Juliann Kiang na amedd army mil Inducible heat shock protein 70 kD HSP 70i has been shown to protect cells tissues and organs from harmful assaults in in vivo and in vitro experimental models Hemorrhagic shock followed by resuscitation is the principal cause of death among trauma patients and soldiers in the battlefield Although the underlying mechanisms are still not fully understood it has been shown that nitric oxide NO overproduction and inducible nitric oxide synthase iNOS over expression play important roles in producing injury caused by hemorrhagic shock including increases in polymorphonuclear neutrophils PMN infiltration to injured tissues and leukotriene B 4 LTB 4 generation Moreover transcription factors responsible for iNOS expression are also altered by hemorrhage and resuscitation It has been evident that either up regulation of HSP 70i or down regulation of iNOS can limit tissue injury caused by ischemia reperfusion or hemorrhage resuscitation In our laboratory geldanamycin a member of ansamycin family has been shown to induce HSP 70i overexpression and then subsequently to inhibit

    Original URL path: http://www.cell-research.com/arts.asp?id=1228 (2016-02-14)
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  • Cell Research
    E mail jqwyin sun5 ibp ac cn RNA interference RNAi is an adaptive defense mechanism triggered by double stranded RNA dsRNA It is a powerful reverse genetic tool that has been widely employed to silence gene expression in mammalian and human cells RNAi based gene therapies especially in viral diseases have become more and more interesting and promising Recently small interfering RNA siRNA can be used to protect host from

    Original URL path: http://www.cell-research.com/arts.asp?id=1229 (2016-02-14)
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  • Cell Research
    plaques are recognized as pathological processes in Alzheimer disease AD brain By using amyloid precursor protein APP transfected cells this study aims to investigate the effect of overproduction of Aβ on cell differentiation and cell viability It was shown that after serum withdrawal untransfected cell N2a Wt and vector transfected cells N2a vector extended long and branched cell processes whereas no neurites was induced in wild type APP N2a APP695 and Swedish mutant APP N2a APPswe transfected N2a cells After differentiation by serum withdrawal the localization of APP Aβ and neurofilament was extended to neurites whereas those of APP transfected cells were still restricted within the cell body Levels of both APP and Aβ were significantly higher in N2a APP695 and N2a APPswe than in N2a Wt as determined by Western blot and Sandwich ELISA respectively To further investigate the effect of Aβ on the inhibition of cell differentiation we added exogenously the similar level or about 10 times of the Aβ level produced by N2a APP695 and N2a APPswe to the culture medium and co cultured with N2a Wt for 12 h and we found that the inhibition of serum withdrawal induced differentiation observed in N2a APP695 and N2a

    Original URL path: http://www.cell-research.com/arts.asp?id=1230 (2016-02-14)
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  • Cell Research
    Yan Chun CHE 1 Jiu Yong XIE 2 1 Institute of Medical Biology Chinese Academy of Medical Sciences and Peking Union Medical College Kunming 650118 China 2 Department of Physiology Faculty of Medicine University of Manitoba 420 BMSB 730 William Ave Winnipeg MB R3E 3J7 Canada Correspondence Jia You CHU Jiu Yong XIE E mail chujy public km yn cn xiej cc umanitoba ca The splicing of many alternative exons in the precursor messenger RNA pre mRNA is regulated by extracellular factors but the underlying molecular bases remain unclear Here we report the differential regulation of Bcl x pre mRNA splicing by extracellular factors and their distinct requirements for pre mRNA elements In K562 leukemia cells treatment with interleukin 6 IL 6 or granulocyte macrophage colony stimulating factor GM CSF reduced the proportion of the Bcl xL variant mRNA while treatment with 12 O tetradecanoylphorbol 13 acetate TPA had no effect In U251 glioma cells however TPA efficiently increased the Bcl xL level These regulations were also seen for a transfected splicing reporter mini gene Further analyses of deletion mutants indicate that nucleotides 1 176 of the downstream intron are required for the IL 6 effect whereas additional nucleotides 177

    Original URL path: http://www.cell-research.com/arts.asp?id=1231 (2016-02-14)
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  • Cell Research
    and Biochemistry Huazhong University of Science and Technology Wuhan 430074 China 2 National Key Laboratory of Biomacromoleculars Institute of Biophysics Chinese Academy of Sciences Beijing 100101 China Correspondence Tao Xu E mail txu mail hust edu cn Glucose transporter 4 GLUT4 is responsible for insulin stimulated glucose transporting into the insulin sensitive fat and muscle cells The dynamics of GLUT4 storage vesicles GSVs remains to be explored and it is unclear how GSVs are arranged based on their mobility We examined this issue in 3T3 L1 cells via investigating the three dimensional mobility of single GSV labeled with EGFP fused GLUT4 A thin layer of cytosol right adjacent to the plasma membrane was illuminated and successively imaged at 5 Hz under a total internal reflection fluorescence microscope with a penetration depth of 136 nm Employing single particle tracking the three dimensional subpixel displacement of single GSV was tracked at a spatial precision of 22 nm Both the mean square displacement and the diffusion coefficient were calculated for each vesicle Tracking results revealed that vesicles moved as if restricted within a cage that has a mean radius of 160 nm suggesting the presence of some intracellular tethering matrix By constructing the

    Original URL path: http://www.cell-research.com/arts.asp?id=1232 (2016-02-14)
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  • Cell Research
    Wen Xin QIN Da Fang WAN Jian Ren GU State Key Laboratory of Oncogenes and related Genes Shanghai Cancer Institute Shanghai Jiao Tong University Medical School 2200Ln 25 Xie Tu Road Shanghai 200032 China Correspondence Da Fang WAN E mail nlorg public sta net cn CT120 a novel membrane associated gene implicated in lung carcinogenesis was previously identified from chromosome 17p13 3 locus a hot mutation spot involved in human malignancies In the present study we further determined that CT120 ectopic expression could promote cell proliferation activity of NIH3T3 cells using MTS assay and monitored the downstream effects of CT120 in NIH3T3 cells with Atlas mouse cDNA expression arrays Among 588 known genes 133 genes were found to be upregulated or downregulated by CT120 Two major signaling pathways involved in cell proliferation cell survival and anti apoptosis were overexpressed and activated in response to CT120 One is the Raf MEK Erk signal cascades and the other is the PI3K Akt signal cascades suggesting that CT120 might contribute at least in part to the constitutively activation of Erk and Akt in human lung caner cells In addition some tumor metastasis associated genes cathepsin B cathepsin D cathepsin L MMP 2 TIMP

    Original URL path: http://www.cell-research.com/arts.asp?id=1233 (2016-02-14)
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  • Cell Research
    2 Hong Guang ZHU 1 1 Department of Pathology Shanghai Medical College Fudan University Shanghai 200032 China 2 Institute of Irradiation Medicine Fudan University Shanghai 200032 China Correspondence Hong Guang ZHU Tel 86 21 54237049 E mail hongguang 701 yahoo com Overexpression and activation of HER 2 neu also known as c erbB 2 a proto oncogene was found in about 30 of human breast cancers promoting cancer growth and making cancer cells resistant to chemo and radio therapy Wild type p53 is crucial in regulating cell growth and apoptosis and is found to be mutated or deleted in 60 70 of human cancers And some cancers with a wild type p53 do not have normal p53 function suggesting that it is implicated in a complex process regulated by many factors In the present study we showed that the overexpression of HER 2 neu could decrease the amount of wild type p53 protein via activating PI3K pathway as well as inducing MDM2 nuclear translocation in MCF7 human breast cancer cells Blockage of PI3K pathway with its specific inhibitor LY294002 caused G1 S phase arrest decreased cell growth rate and increased chemo and radio therapeutic sensitivity in MCF7 cells expressing wild

    Original URL path: http://www.cell-research.com/arts.asp?id=1234 (2016-02-14)
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  • Cell Research
    S Koide 3 1 National Laboratory Medical Molecular Biology Institute of Basic Medical Sciences Chinese Academy of Medical Sciences Peking Union Medical College 5 Dong Dan San Tiao 100005 Beijing China 2 National Research Institute for Family Planning WHO Collaboration Center for Research in Human Reproduction Beijing 12 Da Hui Si 100081 Beijing China 3 Center for Biomedical Research Population Council 1230 York Avenue New York NY 10021 USA 4 Chinese National Human Genome Center Beijing 3 707 North Yong Chang Road BDA Beijing 100176 China Correspondence Lin Fang Wang Tel 86 10 65296418 E mail wanglf ms imicams ac cn The method of laser capture microdissection LCM combined with suppressive subtractive hybridization SSH was developed to isolate specific germ cells from human testis sections and to identify the genes expressed during differentiation and development In the present study over 10 000 primary spermatocytes and round spermatid cells were successfully isolated by LCM Using the cDNAs from primary spermatocytes and round spermatids SSH cDNAs library of primary spermatocyte specific was constructed The average insert size of the cDNA isolated from 75 randomly picked white clones was 500 bp ranging from 250 bp to 1 7 kb Using the dot blot

    Original URL path: http://www.cell-research.com/arts.asp?id=1235 (2016-02-14)
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