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  • Cell Research
    Top 10 VOLUME 15 ISSUE 2 2 2005 92 96 Krüppel like factors 4 and 5 the yin and yang regulators of cellular proliferation Amr M GHALEB 1 Mandayam O NANDAN 1 Sengthong CHANCHEVALAP 1 W Brian DALTON 1 Irfan M HISAMUDDIN 1 Vincent W YANG 1 2 1 Division of Digestive Diseases Department of Medicine Emory University School of Medicine Atlanta Georgia USA 2 Department of Hematology and Oncology Winship Cancer Institute Emory University School of Medicine Atlanta Georgia USA Correspondence Vincent W Yang Tel 404 727 5638 E mail vyang emory edu Krüppel like factors KLFs are evolutionarily conserved zinc finger containing transcription factors with diverse regulatory functions in cell growth proliferation differentiation and embryogenesis KLF4 and KLF5 are two closely related members of the KLF family that have a similar tissue distribution in embryos and adults However the two KLFs often exhibit opposite effects on regulation of gene transcription despite binding to similar if not identical cis acting DNA sequences In addition KLF4 and 5 exert contrasting effects on cell proliferation in many instances while KLF4 is an inhibitor of cell growth KLF5 stimulates proliferation Here we review the biological properties and biochemical mechanisms of action of

    Original URL path: http://www.cell-research.com/arts.asp?id=1204 (2016-02-14)
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  • Cell Research
    Sciences Shanghai Institute of Biological Sciences Chinese Academy of Sciences Shanghai China 3 NHGG Bio X Center Shanghai Jiao Tong University Shanghai China Correspondence Lin He Tel 86 21 62822491 E mail helin nhgg org In multiloci based genetic association studies of complex diseases a powerful and high efficient tool for analyses of linkage disequilibrium LD between markers haplotype distributions and many chi square p values with a large number

    Original URL path: http://www.cell-research.com/arts.asp?id=1205 (2016-02-14)
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  • Cell Research
    Politécnico Nacional CINVESTAV IPN México 2 Centro de Investigación en Ciencias Biológicas Universidad Autónoma de Tlaxcala México Correspondence Juan B Kouri Tel 5255 50613800 ext 3343 E mail bkouri mail cinvestav mx The aim of this work was to study the ontogeny of chondrocyte cell division using embryo adult and osteoarthritic OA cartilage We searched for mitosis phases and performed a comparative evaluation of mitotic index basic fibroblast growth factor b FGFb transforming growth factor β1 TGF β1 receptors cyclin dependent kinase CDK1 and Cyclin B expression in fetal neonate 3 5 8 weeks old rats and experimental OA Our results showed that mitosis phases were observed in all normal cartilage studied although we found a decrease in mitotic index in relation to tissue development No mitosis was detected in OA cartilage We also found a statistical significant reduction in cell number in OA cartilage compared with the normal tissue Furthermore FGFb and TGF β1 receptors diminished in relation to tissue development and were very scarce in experimental OA Western blot assays showed CDK 1 expression in all cases including human OA cartilage Similar results were observed for Cyclin B except for 8 weeks when it was not expressed Our

    Original URL path: http://www.cell-research.com/arts.asp?id=1206 (2016-02-14)
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  • Cell Research
    ying Zhi 1 Fang Ding 1 Ai ping Luo 1 Zhi hua Liu 1 1 National Laboratory of Molecular Oncology Cancer Institute Chinese Academy of Medical Sciences Peking Union Medical College Beijing 100021 China 2 Life Science Institute Hebei Normal University Shijiazhuang 050016 China Correspondence Zhi hua Liu Tel 86 10 87788490 E mail liuzh pubem cicams ac cn Epidermal type transglutaminase 3 TGM3 is involved in the cross linking of structural proteins to form the cornified envelope in the epidermis In the present study we detected the expression of TGM3 in the mouse embryo using RT PCR TGM3 mRNA is weakly presented from E11 5 to E14 5 and increases significantly from E15 5 to birth Then we determined the spatial and temporal expression pattern of TGM3 in the skin and other organs by in situ hybridization We found a deprivation of TGM3 in skin at E11 5 while a rich supply in periderm cells and a weak expression in basal cells from E12 5 to E14 5 From the period of E15 5 to E16 5 after keratinization in the epidermis TGM3 was expressed in the granular and cornified layers The electron microscopic observation of the C57BL 6J

    Original URL path: http://www.cell-research.com/arts.asp?id=1207 (2016-02-14)
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  • Cell Research
    Research and the Canadian Institutes of Health Research Group in Lung Development Hospital for Sick Children Toronto Canada M5G 1X8 2 Department of Paediatrics University of Toronto Toronto Canada M5S 1A1 3 Department of Physiology University of Toronto Toronto Canada M5S 1A1 4 Laboratory Medicine and Pathobiology University of Toronto Toronto Canada M5S 1A1 5 Departments of Pediatrics The Johns Hopkins University School of Medicine Baltimore MD 21287 USA Correspondence Jim HU Tel 01 416 813 6412 E mail jhu sickkids on ca Interleukin IL 8 is a potent neutrophil chemotactic factor and a crucial mediator in neutrophil dependent inflammation Various cell types produce IL 8 either in response to external stimuli such as cytokines or bacterial infection or after malignant transformation Anti IL 8 strategies have been considered for anti inflammatory therapy In this paper we demonstrate that the RNA interference technique can be used to efficiently down regulate IL 8 protein expression in airway epithelial cells We used a helper dependent adenoviral vector to express a small hairpin sh RNA targeting human IL 8 in cultured airway epithelial cells IB3 1 Cftr C38 Cftr corrected stimulated with TNF α IL 1β or heat inactivated Burkholderia cenocepacia Stimulated IL

    Original URL path: http://www.cell-research.com/arts.asp?id=1208 (2016-02-14)
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  • Cell Research
    molecular biology College of Life Sciences Nankai University Tianjin 300071 China Correspondence Xi Tai HUANG Tel 86 22 23508874 E mail xthuang public tpt tj cn The dynamic distribution of phosphorylated Histone H3 on Ser10 phospho H3 in cells was investigated to determine its function during mitosis Human breast adenocarcinoma cells MCF 7 and Chinese hamster cells CHO were analyzed by indirect immunofluorescence staining with an antibody against phospho H3 We found that the phosphorylation begins at early prophase and spreads throughout the chromosomes at late prophase At metaphase most of the phospho H3 aggregates at the end of the condensed entity of chromosomes at equatorial plate During anaphase and telophase the fluorescent signal of phospho H3 is detached from chromosomes into cytoplasm At early anaphase phospho H3 shows ladder bands between two sets of separated chromosome and forms sandwich like structure when the chromosomes condensed With the cleavage progressing the ladders of the histone contract into a bigger bright dot Then the histone aggregates and some of compacted microtubules in the midbody region are composed into a bar like complex to separate daughter cells The daughter cells seal their plasma membrane along with the ends of the bar inside

    Original URL path: http://www.cell-research.com/arts.asp?id=1209 (2016-02-14)
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  • Cell Research
    pronuclei reside in the same zygotic cytoplasm they differ in many respects such as volume and transcriptional activity The aim of this study is to investigate whether these differences are lost during the first mitosis For this purpose a new method was developed to inhibit the mixing of two parental chromosomes during mitosis thus to induce the formation of two nuclei after they exit from the mitotic phase In this method one cell embryos are arrested at metaphase by treatment with nocodazole and whn exitting from the mitotic phase two nuclei were formed in a single karyocyte following treatment with 6 dimethylaminopurine 6 DMAP These embryos were designated as post mitotic embryos PM embryos in which the two nuclei were derived from the male and female genomes We found that in the control one cell embryos that had not been treated with the reagents the volume of the male pronucleus was about 1 65 fold greater than that of the female pronucleus whereas the volumes of the two nuclei in the PM embryos were similar volume ratio of 1 01 Although a two fold difference in transcriptional activity was detected between the male and female pronuclei in the control embryos

    Original URL path: http://www.cell-research.com/arts.asp?id=1210 (2016-02-14)
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  • Cell Research
    1 Department of Biochemistry Ministry of Education Key laboratory for Biodiversity Science and Ecological Engineering School of Life Sciences Fudan University 220 Handan Road Shanghai 200433 China 2 School of Horticulture and Gardening Southwest Forestry College Kun Ming 650224 China 3 Department of biology School of Life and Environment Science Shanghai Normal University Shanghai 200234 China 4 Fudan Centre for Genetic Diversity and designed Agriculture Fudan University 220 Handan Road Shanghai 200433 China Correspondence Ben Ke Kuai Tel 86 21 65643676 E mail bkkuai fudan edu cn A developmentally retarded mutant drm1 was identified from ethyl methanesulfonate EMS mutagenized M2 seeds in Columbia Col 0 genetic background The drm1 flowers 109 d after sowing with a whole life cycle of about 160 d It also shows a pleiotropic phenotype e g slow germination and lower germination rate lower growth rate curling leaves and abnormal floral organs The drm1 mutation was a single recessive nuclear mutation which was mapped to the bottom of chromosome 5 and located within a region of 20 30 kb around MXK3 1 There have been no mutants with similar phenotypes reported in the literature suggesting that DRM1 is a novel flowering promoting locus The findings that the drm1 flowered lately under all photoperiod conditions and its late flowering phenotype was significantly restored by vernalization treatment suggest that the drm1 is a typical late flowering mutant and most likely associated with the autonomous flowering pathway The conclusion was further confirmed by the revelation that the transcript level of FLC was constantly upregulated in the drm1 at all the developmental phases examined except for a very early stage Moreover the transcript levels of two other important repressors EMF and TFL1 were also upregulated in the drm1 implying that the two repressors along with FLC seems to act in

    Original URL path: http://www.cell-research.com/arts.asp?id=1211 (2016-02-14)
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