archive-com.com » COM » C » CELL-RESEARCH.COM

Total: 1754

Choose link from "Titles, links and description words view":

Or switch to "Titles and links view".
  • Cell Research

    (No additional info available in detailed archive for this subpage)
    Original URL path: /artsmore1.asp?id=101 (2016-02-14)



  • Cell Research
    cycle to mitosis is a tightly regulated cellular process that requires activation of the Cdc2 kinase which determines onset of mitosis in all eukaryotic cells In both human and fission yeast Schizosaccharomyces pombe cells the activity of Cdc2 is regulated in part by the phosphorylation status of tyrosine 15 Tyr15 on Cdc2 which is phosphorylated by Wee1 kinase during late G2 and is rapidly dephosphorylated by the Cdc25 tyrosine phosphatase to trigger entry into mitosis These Cdc2 regulators are the downstream targets of two well characterized G2 M checkpoint pathways which prevent cells from entering mitosis when cellular DNA is damaged or when DNA replication is inhibited Increasing evidence suggests that Cdc2 is also commonly targeted by viral proteins which modulate host cell cycle machinery to benefit viral survival or replication In this review we describe the effect of viral protein R Vpr encoded by human immunodeficiency virus type 1 HIV 1 on cell cycle G2 M regulation Based on our current knowledge about this viral effect we hypothesize that Vpr induces cell cycle G2 arrest through a mechanism that is to some extent different from the classic G2 M checkpoints One the unique features distinguishing Vpr induced G2 arrest

    Original URL path: http://www.cell-research.com/arts.asp?id=1192 (2016-02-14)
    Open archived version from archive

  • Cell Research
    Institutes of Health Bethesda MD 20892 USA Correspondence Yun Bo SHI Tel 301 402 1004 E mail Shi helix nih gov The matrix metalloproteinase MMP stromelysin 3 ST3 has long been implicated to play an important role in extracellular matrix ECM remodeling and cell fate determination during normal and pathological processes However like other MMPs the molecular basis of ST3 function in vivo remains unclear due to the lack of information on its physiological substrates Furthermore ST3 has only weak activities toward all tested ECM proteins Using thyroid hormone dependent Xenopus laevis metamorphosis as a model we demonstrated previously that ST3 is important for apoptosis and tissue morphogenesis during intestinal remodeling Here we used yeast two hybrid screen with mRNAs from metamorphosing tadpoles to identify potential substrate of ST3 during development We thus isolated the 37 kd laminin receptor precursor LR We showed that LR binds to ST3 in vitro and can be cleaved by ST3 at two sites distinct from where other MMPs cleave Through peptide sequencing we determined that the two cleavage sites are in the extracellular domain between the transmembrane domain and laminin binding sequence Furthermore we demonstrated that these cleavage sites are conserved in human LR

    Original URL path: http://www.cell-research.com/arts.asp?id=1193 (2016-02-14)
    Open archived version from archive

  • Cell Research
    Top 10 VOLUME 15 ISSUE 3 3 2005 160 166 Overexpression of Bax sensitizes prostate cancer cells to TGF β induced apoptosis Pei Hui LIN 1 Zui PAN 1 Lin ZHENG 1 Na LI 1 David DANIELPOUR 2 Jian Jie MA 1 1 Department of Physiology and Biophysics Robert Wood Johnson Medical School 675 Hoes Lane Piscataway NJ 08854 USA 2 Ireland Cancer Center Case Western Reserve University 10900 Euclid Avenue Cleveland OH 44106 USA Correspondence Jian Jie MA Tel 732 235 4494 E mail maj2 umdnj edu NRP 154 is a tumorigenic epithelial cell line derived from the preneoplastic dorsal lateral prostate of rats These cells are exquisitely sensitive to TGF β induced apoptosis In contrast we find that NRP 154 cells can sustain overexpression of exogenous Bax protein which is different from non tumor cells where Bax functions as a ubiquitous stimulator of apoptosis NRP 154 cells stably overexpressing Bax show increased sensitivity to TGF β induced apoptosis The degree of TGF β induced apoptosis displays high correlation with cleavage of Bax at the amino terminus Our data indicate that prostate cancer cells can host high levels of latent Bax which can be activated through post translational modification

    Original URL path: http://www.cell-research.com/arts.asp?id=1194 (2016-02-14)
    Open archived version from archive

  • Cell Research
    86 21 63846590 776541 ext E mail Lian Fan online sh cn Human leukocyte antigen G HLA G has long been speculated as a beneficial factor for a successful pregnancy for its restricted expression on fetal maternal extravillous cytotrophoblasts and its capability of modulating uterine natural killer cell uNK function such as cytotoxicity and cytokine production through NK cell receptors HLA class I α1 domain is an important killer cell Ig like receptor KIR recognition site and the Met 76 and Gln 79 are unique to HLA G in this region NK cell receptor KIR2DL4 is a specific receptor for HLA G yet the recognition site on HLA G remains unknown In this study retroviral transduction was applied to express the wild type HLA G HLA wtG mutant HLA G HLA mG on the chronic myelogenous leukemia cell line K562 cells and KIR2DL4 molecule on NK 92 cells respectively KIR2DL4 IgG Fc fusion protein was generated to determine the binding specificity between KIR2DL4 and HLA G Our results showed that residue Met 76 Gln 79 mutated to Ala 76 79 in the α1 domain of HLA G protein could affect the binding affinity between KIR2DL4 and HLA G meanwhile the

    Original URL path: http://www.cell-research.com/arts.asp?id=1196 (2016-02-14)
    Open archived version from archive

  • Cell Research
    VOLUME 15 ISSUE 3 3 2005 183 186 Golgi localization and dynamics of hyaluronan binding protein 1 HABP1 p32 C1QBP during the cell cycle Aniruddha SENGUPTA 1 Bhaswati BANERJEE 1 Rakesh K TYAGI 2 Kasturi DATTA 1 2 1 Biochemistry Laboratory School of Environmental Sciences Jawaharlal Nehru University New Delhi 110067 India 2 Special Centre for Molecular Medicine Jawaharlal Nehru University New Delhi India Correspondence Kasturi DATTA Tel 91 11 26172438 or 26704327 E mail kdatta mail jnu ac in Hyaluronan binding protein 1 HABP1 is a negatively charged multifunctional mammalian protein with a unique structural fold Despite the fact that HABP1 possesses mitochondrial localization signal it has also been localized to other cellular compartments Using indirect immunofluorescence we examined the sub cellular localization of HABP1 and its dynamics during mitosis We wanted to determine whether it distributes in any distinctive manner after mitotic nuclear envelope disassembly or is dispersed randomly throughout the cell Our results reveal the golgi localization of HABP1 and demonstrate its complete dispersion throughout the cell during mitosis This distinctive distribution pattern of HABP1 during mitosis resembles its ligand hyaluronan suggesting that in concert with each other the two molecules play critical roles in this dynamic

    Original URL path: http://www.cell-research.com/arts.asp?id=1197 (2016-02-14)
    Open archived version from archive

  • Cell Research
    PENG 1 3 Zhong Hai LI 2 Hui XIANG 1 3 Jian Hua HUANG 1 3 Shi Hai JIA 1 3 Xue Xia MIAO 1 Yong Ping HUANG 1 1 Institute of Plant Physiology and Ecology Shanghai Institute for Biological Sciences Graduate School of the Chinese Academy of Sciences Chinese Academy of Sciences 300 Fenglin Road Shanghai 200032 China 2 Chinese National Human Genome Center at Shanghai 250 Bibo Road Shanghai 201203 China 3 Graduate School of the Chinese Academy of Sciences Beijing 100039 China Correspondence Yong Ping HUANG Tel 86 21 54924047 E mail yongping iris sipp ac cn We compared the expression patterns of three representative genes in undamaged tomato and tobacco plants in response to exposure to either tomato or tobacco fed on by Helicoverpa armigera cotton bollworm When tomato and tobacco two species of one family were incubated in the chambers with the tomato plants damaged by the cotton bollworm the expression of the PR1 BGL2 and PAL genes was up regulated in leaves of both plants However the levels of gene expression were significantly higher in the tomato than that in the tobacco In addition the activities of enzymes peroxidase polyphenol oxidase and lipoxygenase were

    Original URL path: http://www.cell-research.com/arts.asp?id=1198 (2016-02-14)
    Open archived version from archive

  • Cell Research
    Molecular Biology Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai 200031 China Correspondence You Xin JIN Tel 86 21 54921222 E mail yxjin sibs ac cn RNA interference RNAi is triggered by the presence of a double stranded RNA dsRNA and results in the silencing of homologous gene expression through the specific degradation of an mRNA containing the same sequence dsRNA mediated RNAi can be used in a wide variety of eucaryotes to induce the sequence specific inhibition of gene expression Synthetic 21 23 nucleotide nt small interfering RNA siRNA with 2 nt 3 overhangs was recently found to mediate efficient sequence specific mRNA degradation in mammalian cells Here we studied the effects of synthetic siRNA duplexes targeted to SARS coronavirus structural proteins E M and N in a cell culture system Among total 26 siRNA duplexes we obtained 3 siRNA duplexes which could sequence specifically reduce target genes expression over 80 at the concentration of 60 nM in Vero E6 cells The downregulation effect was in correlation with the concentrations of the siRNA duplexes in a range of 0 60 nM Our results also showed that many inactive siRNA duplexes may

    Original URL path: http://www.cell-research.com/arts.asp?id=1199 (2016-02-14)
    Open archived version from archive



  •