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  • Cell Research
    2 Hui LI 1 Lin Yan ZHU 2 Pan LI 1 Ping ZHONG 2 Si Huai NIE 2 Li Xin CHEN 1 3 1 Laboratory of Cell Biology 2 Laboratory of Physiology Guangdong Medical College Zhanjiang 524023 China 3 Cardiff School of Biosciences Cardiff University Biomedical Sciences Building Museum Avenue Cardiff CF10 3US UK Correspondence Li Xin CHEN Tel 44 29 20874088 E mail CHENL1 cardiff ac uk The transwell chamber migration assay and CCD digital camera imaging techniques were used to investigate the relationship between regulatory volume decrease RVD and cell migration in nasopharyngeal carcinoma cells CNE 2Z cells Both migrated and non migrated CNE 2Z cells when swollen by 47 hypotonic solution exhibited RVD which was inhibited by extracellular application of chloride channel blockers adenosine 5 triphosphate ATP 5 nitro 2 3 phenylpropylamino benzoic acid NPPB and tamoxifen However RVD rate in migrated CNE 2Z cells was bigger than that of non migrated cells and the sensitivity of migrated cells to NPPB and tamoxifen was higher than that of non migrated cells ATP NPPB and tamoxifen also inhibited migration of CNE 2Z cells The inhibition of migration was positively correlated to the blockage of RVD with a correlation

    Original URL path: http://www.cell-research.com/arts.asp?id=1178 (2016-02-14)
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  • Cell Research
    394 400 Human embryonic stem cell lines derived from the Chinese population Zhen Fu FANG 1 2 Fan JIN 3 Hui GAI 1 Ying CHEN 1 Li WU 1 Ai Lian LIU 1 Bin CHEN 1 Hui Zhen SHENG 1 2 1 2 Laboratory of Stem Cell Biology Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai 200031 China Correspondence Hui Zhen SHENG Tel 86 21 55570017 E mail hzsheng sh163a sta net cn Six human embryonic stem cell lines were established from surplus blastocysts The cell lines expressed alkaline phosphatase and molecules typical of primate embryonic stem cells including Oct 4 Nanog TDGF1 Sox2 EBAF Thy 1 FGF4 Rex 1 SSEA 3 SSEA 4 TRA 1 60 and TRA 1 81 Five of the six lines formed embryoid bodies that expressed markers of a variety of cell types four of them formed teratomas with tissue types representative of all three embryonic germ layers These human embryonic stem cells are capable of producing clones of undifferentiated morphology and one of them was propagated to become a subline Human embryonic stem cell lines from the Chinese population should facilitate stem cell research and may

    Original URL path: http://www.cell-research.com/arts.asp?id=1181 (2016-02-14)
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  • Cell Research
    405 In vitro cultivation and differentiation of fetal liver stem cells from mice Ren Qing FENG Li Ying DU Zhen Quan GUO College of Life Sciences Peking University Beijing 100871 China Correspondence Ren Qing FENG Tel 86 10 62757161 E mail rqfeng pku edu cn During embryonic development pluripotent endoderm tissue in the developing foregut may adopt pancreatic fate or hepatic fate depending on the activation of key developmental regulators Transdifferentiation occurs between hepatocytes and pancreatic cells under specific conditions Hepatocytes and pancreatic cells have the common endodermal progenitor cells In this study we isolated hepatic stem progenitor cells from embryonic day ED 12 14 Kun Ming mice with fluorescence activated cell sorting FACS The cells were cultured under specific conditions The cultured cells deploy dithizone staining and immunocytochemical staining at the 15th 30th and 40th day after isolation The results indicated the presence of insulin producing cells When the insulin producing cells were transplanted into alloxan induced diabetic mice the nonfasting blood glucose level was reduced These results suggested that fetal liver stem progenitor cells could be converted into insulin producing cells under specific culture conditions Fetal liver stem progenitor cells could become the potential source of insulin producing

    Original URL path: http://www.cell-research.com/arts.asp?id=1182 (2016-02-14)
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  • Cell Research

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    Original URL path: /artsmore1.asp?id=98 (2016-02-14)


  • Cell Research
    Zheng 2 Hong Tang 2 Genhong Cheng 1 2 1 Department of Microbiology Immunology and Molecular Genetics University of California Los Angeles Los Angeles CA 90095 USA 2 Center for Infection and Immunity Institute of Biophysics Chinese Academy of Sciences Beijing 100101 China Correspondence G Cheng Tel 310 825 8896 E mail genhongc microbio ucla edu Type I interferons IFN are well studied cytokines with anti viral and immune modulating functions Type I IFNs are produced following viral infections but until recently the mechanisms of viral recognition leading to IFN production were largely unknown Toll like receptors TLRs have emerged as key transducers of type I IFN during viral infections by recognizing various viral components Furthermore much progress has been made in defining the signaling pathways downstream of TLRs for type I IFN production TLR7 and TLR9 have become apparent as universally important in inducing type I IFN during infection with most viruses particularly by plasmacytoid dendritic cells New intracellular viral pattern recognition receptors leading to type I IFN production have been identified Many bacteria can also induce the up regulation of these cytokines Interestingly recent studies have found a detrimental effect on host cells if type I IFN is

    Original URL path: http://www.cell-research.com/arts.asp?id=1165 (2016-02-14)
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  • Cell Research
    18 intron 1 Deanna YANIW 1 2 Jim HU 1 2 3 4 1 Programme in Lung Biology Research and the Canadian Institutes of Health Research Group in Lung Development Hospital for Sick Children Toronto Canada M5G 1X8 2 Institute of Medical Science University of Toronto Toronto Canada M5S 1A1 3 Departments of Paediatrics University of Toronto Toronto Canada M5S 1A1 4 Laboratory Medicine and Pathbiology University of Toronto Toronto Canada M5S 1A1 Correspondence Jim HU Tel 01 416 813 6412 E mail jhu sickkids on ca The role of Ese 2 an Ets family transcription factor in gene regulation is not known In this study the interaction between Ese 2 and cytokeratin 18 K18 intron 1 was characterized in lung epithelial cells Reporter gene assays showed Ese 2 was able to upregulate K18 intron 1 enhanced reporter gene expression by approximately 2 fold We found that full length Ese 2 did not bind DNA strongly therefore truncated versions of the protein containing the ETS domain or Pointed domain were created and tested in electrophoresis mobility shift assays Multiple interactions between the ETS domain and putative DNA binding sites within K18 intron 1 were observed which led to the determination

    Original URL path: http://www.cell-research.com/arts.asp?id=1166 (2016-02-14)
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  • Cell Research
    John Radcliffe Hospital Oxford OX3 9DS UK 3 Hammersmith Hospital Du Cane Road London W12 0NN UK 4 Department of Immunology Fourth Military Medical University Xi an 710032 China Correspondence Gavin R SCREATON Tel 44 1865 222442 E mail g screaton imperial ac uk TRAIL tumor necrosis factor related apoptosis inducing ligand is a member of the TNF family of proteins Tumour cells were initially found to have increased sensitivity to TRAIL compared with normal cells raising hopes that TRAIL would prove useful as an anti tumor agent The production of reliable monoclonal antibodies against TRAIL and its receptors that can stain fixed specimens will allow a thorough analysis of their expression on normal and malignant tissues Here we report the generation of monoclonal antibodies against TRAIL and its four membrane bound receptors TR1 4 which have been used to stain a range of normal and malignant cells as routinely fixed specimens Low levels of TRAIL expression were found to be limited mostly to smooth muscle in lung and spleen as well as glial cells in the cerebellum and follicular cells in the thyroid Expression of the TRAIL decoy receptors TR3 and 4 was not as widespread as indicated by

    Original URL path: http://www.cell-research.com/arts.asp?id=1167 (2016-02-14)
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  • Cell Research
    in breast cancer cells Tyler Zarubin Qing Jing Liguo New Jiahuai Han Department of Immunology The Scripps Research Institute 10550 North Torrey Pines Road La Jolla CA 92037 USA Correspondence Jiahuai HAN Tel 01 858 784 8704 E mail jhan scripps edu Although the antiestrogen agent tamoxifen has long been used to treat women with hormone receptor positive invasive breast carcinoma the mechanisms of its action and acquired resistance to tamoxifen during treatment are largely unknown A number of studies have revealed that over activation of some signaling pathways can cause tamoxifen resistance however very little information is available regarding the genes whose loss of function alternation contribute to tamoxifen resistance Here we used a forward genetic approach in vitro to generate tamoxifen resistant cells from the tamoxifen sensitive breast cancer cell line ZR 75 1 and further identified the disrupted gene in different tamoxifen resistant clones Retinol binding protein 7 DNA polymerase transactivated protein 3 γ glutamyltransferase like activity 1 slit robo RhoGTPase activating protein tetraspan NET 4 HSPC194 amiloride sensitive epithelial sodium channel gene and Notch2 were the eight mutated genes identified in different tamoxifen resistant clones suggesting their requirement for tamoxifen sensitivity in ZR 75 1 cells

    Original URL path: http://www.cell-research.com/arts.asp?id=1168 (2016-02-14)
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