archive-com.com » COM » C » CELL-RESEARCH.COM

Total: 1754

Choose link from "Titles, links and description words view":

Or switch to "Titles and links view".
  • Cell Research
    15 ISSUE 7 7 2005 511 522 Gene expression alteration during redox dependent enhancement of arsenic cytotoxicity by emodin in HeLa cells Xiao Jing WANG Jie YANG Hui CANG Yan Qiong ZOU Jing YI Department of Cell Biology Shanghai Second Medical University 280 Chongqing Road Shanghai 200025 China Correspondence Jing YI Tel 86 21 63846590 E mail yijing shsmu edu cn Emodin 1 3 8 trihydroxy 6 methylanthraquinone could enhance the sensitivity of tumor cells to arsenic trioxide As 2 O 3 induced apoptosis via generation of ROS but the molecular mechanism has not been elucidated Here we carried out cDNA microarray based global transcription profiling of HeLa cells in response to As 2 O 3 emodin cotreatment comparing with As 2 O 3 only treatment The results showed that the expression of a number of genes was substantially altered at two time points These genes are involved in different aspects of cell function In addition to redox regulation and apoptosis ROS affect genes encoding proteins associated with cell signaling organelle functions cell cycle cytoskeleton etc These data suggest that based on the cytotoxicity of As 2 O 3 emodin mobilize every genomic resource through which the As 2 O

    Original URL path: http://www.cell-research.com/arts.asp?id=1161 (2016-02-14)
    Open archived version from archive


  • Cell Research
    Tel 421 2 54 77 44 05 E mail usrdokru savba sk Insufficient growth and rarefaction of capillaries followed by endothelial dysfunction may represent one of the most critical mechanisms involved in heart damage In this study we examined histochemical and ultrastructural changes in myocardial capillary endothelium in two models of heart failure streptozotocin induced diabetes mellitus STZ and NO deficient hypertension in male Wistar rats Diabetes was induced by a single i v dose of STZ 45 mg kg and chronic 9 week stage was analysed To induce NO deficient hypertension animals were treated with inhibitor of NO synthase L nitroarginine methylester L NAME 40 mg kg for 4 weeks Left ventricular tissue was processed for enzyme catalytic histochemistry of capillary alkaline phosphatase AlPh dipeptidyl peptidase IV DPP IV and endothelial NO synthase NADPH diaphorase NOS and for ultrastructural analysis In diabetic and hypertensive rats lower absent AlPh and DPP IV activities were found in focal micro areas NOS activity was significantly reduced and persisted only locally Quantitative evaluation demonstrated reduction of reaction product intensity of AlPh DPP and NOS by 49 50 74 36 20 05 in diabetic and 62 93 82 71 37 65 in hypertensive rats

    Original URL path: http://www.cell-research.com/arts.asp?id=1162 (2016-02-14)
    Open archived version from archive

  • Cell Research
    Gynecology and Obstetrics hospital Affiliate of Capital University of Medical Sciences Beijing 100026 China 2 Department of Gynecology and Obstetrics Second Hospital of Jilin University Changchun 130021 China 3 Department of Cell Biology Institute of Basic Medical Sciences Academy of Military Medical Sciences Beijing 100850 China Correspondence Wei Yuan ZHANG Tel 86 10 85976699 8082 E mail zhangwy9921 hotmail com Human placenta derived mononuclear cells MNC were isolated by a Percoll density gradient and cultured in mesenchymal stem cell MSC maintenance medium The homogenous layer of adherent cells exhibited a typical fibroblast like morphology a large expansive potential and cell cycle characteristics including a subset of quiescent cells In vitro differentiation assays showed the tripotential differentiation capacity of these cells toward adipogenic osteogenic and chondrogenic lineages Flow cytometry analyses and immunocytochemistry stain showed that placental MSC was a homogeneous cell population devoid of hematopoietic cells which uniformly expressed CD29 CD44 CD73 CD105 CD166 laminin fibronectin and vimentin while being negative for expression of CD31 CD34 CD45 and a smooth muscle actin Most importantly immuno phenotypic analyses demonstrated that these cells expressed class I major histocompatibility complex MHC I but they did not express MHC II molecules Additionally these cells could

    Original URL path: http://www.cell-research.com/arts.asp?id=1163 (2016-02-14)
    Open archived version from archive

  • Cell Research
    10 VOLUME 15 ISSUE 7 7 2005 548 552 Calmodulin regulates the post anaphase reposition of centrioles during cytokinesis Yue Yue YU Gu DAI Fei Yan PAN Jie CHEN Chao Jun LI Jiangsu Key Laboratory for Molecular Medical Biotechnology School of Life Sciences Nanjing Normal University Nanjing 210097 China Correspondence Chao Jun LI Tel 86 25 83598812 E mail licj njnu edu cn A transient postanaphase repositioning of the centriole is found to control the completion of cytokinesis Using a green fluorescent protein calmodulin fusion protein as a living cell probe we have previously found that calmodulin is associated with the initiation and progression of cytokinesis In this study we further studied the effect of calmodulin on the repositioning of the centriole and subsequent cell cycle progression When activity of calmodulin is inhibited the regression of the centriole from the intercellular bridge to the cell center is blocked and thus the completion of cell division is repressed and two daughter cells are linked by longer cell bridge in perturbed cells W7 treatment during cytokinesis also results in unfinished cytokinesis and stopped G 1 phase These results suggest that calmodulin activity is required for centriole repositioning and can affect the completion

    Original URL path: http://www.cell-research.com/arts.asp?id=1164 (2016-02-14)
    Open archived version from archive

  • Cell Research

    (No additional info available in detailed archive for this subpage)
    Original URL path: /artsmore1.asp?id=96 (2016-02-14)


  • Cell Research
    Carla R Lyerly Linebarger 1 William B Gurley 2 Robert J Ferl 1 1 Program in Plant Cellular and Molecular Biology Department of Horticultural Sciences University of Florida Gainesville FL 32611 USA 2 Department of Microbiology and Cell Science University of Florida Gainesville FL 32611 USA Correspondence Robert J Ferl Tel 352 392 1928 E mail robferl ufl edu Environmental control of the alcohol dehydrogenase Adh and other stress response genes in plants is in part brought about by transcriptional regulation involving the G box cis acting DNA element and bZIP G box Binding Factors GBFs The mechanisms of GBF regulation and requirements for additional factors in this control process are not well understood In an effort to identify potential GBF binding and control partners maize GBF1 was used as bait in a yeast two hybrid screen of an A thaliana cDNA library GBF Interacting Protein 1 GIP1 arose from the screen as a 496 amino acid protein with a predicted molecular weight of 53 748 kDa that strongly interacts with GBFs Northern analysis of A thaliana tissue suggests a 1 8 1 9 kb GIP1 transcript predominantly in roots Immunolocalization studies indicate that GIP1 protein is mainly localized to the nucleus In vitro electrophoretic mobility shift assays using an Adh G box DNA probe and recombinant A thaliana GBF3 or maize GBF1 showed that the presence of GIP1 resulted in a tenfold increase in GBF DNA binding activity without altering the migration suggesting a transient association between GIP1 and GBF Addition of GIP1 to intentionally aggregated GBF converted GBF to lower molecular weight macromolecular complexes and GIP1 also refolded denatured rhodanese in the absence of ATP These data suggest GIP1 functions to enhance GBF DNA binding activity by acting as a potent nuclear chaperone or crowbar and potentially regulates

    Original URL path: http://www.cell-research.com/arts.asp?id=1147 (2016-02-14)
    Open archived version from archive

  • Cell Research
    WU Tel 86 10 6273 1103 E mail wuwh public3 bta net cn Although there were reports suggesting the involvement of endogenous cAMP in plant defense signaling cascades there is no direct evidence supporting this notion yet and the detailed mechanism is unclear In the present study we have used pathogenic fungi Verticillium dahliae and Arabidopsis plants as a model system of plant microb interaction to demonstrate the function of endogenous cAMP in Arabidopsis defense responses Both V dahliae inoculation and Verticillium toxins injection induced typical wilt symptoms in Arabidopsis seedlings When either 8 Br AMP a membrane permeable cAMP analogue or salicylic acid SA was applied to Arabidopsis the plants became resistant to V dahliae toxins However addition of 8 Br AMP did not increase the resistance of Arabidopsis transgenic plants deficient in SA to the toxins suggesting that cAMP might act upstream of SA in plant defense signaling pathway Indeed 8 Br cAMP and forskolin an activator of adenylyl cyclase significantly stimulated the endogenous SA level in plants whereas DDA an inhibitor of adenylyl cyclase dramatically reduced toxin induced SA increase Both the endogenous cAMP and SA increased significantly in Arabidopsis seedlings treated with toxins Furthermore transcription level

    Original URL path: http://www.cell-research.com/arts.asp?id=1149 (2016-02-14)
    Open archived version from archive

  • Cell Research
    Key Laboratory of Plant Genomics Institute of Genetics and Developmental Biology Chinese Academy of Sciences Beijing 100101 China 2 Graduate School of the Chinese Academy of Sciences Yuquan Road Beijing 100039 China 3 China National Rice Research Institute Chinese Academy of Agricultural Sciences Hangzhou 310006 China Correspondence Chengcai Chu Tel 86 10 64877570 E mail ccchu genetics ac cn WRKY family proteins are a class of plant specific transcription factors that involve in many stress response pathways It has been shown that one Arabidopsis WRKY protein AtWRKY29 22 is activated by MAP kinase signaling cascade and confers resistance to both bacterial and fungal pathogens However little is known about the biological roles of WRKY proteins in rice In this study we investigated the expression patterns of rice AtWRKY29 22 homolog OsWRKY03 under different conditions and also its possible role involved in plant defense Our results showed that OsWRKY03 was up regulated by several defense signaling molecules or different treatments Further analysis revealed that the expression of OsWRKY03 was light dependent Transcriptional activation activity of OsWRKY03 was also demonstrated by yeast functional assay Transient expression of OsWRKY03 GFP fusion protein in onion epidermis cells showed that OsWRKY03 was a nuclear localized

    Original URL path: http://www.cell-research.com/arts.asp?id=1150 (2016-02-14)
    Open archived version from archive



  •