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  • Cell Research
    Developmental Biology College of Life Sciences Wuhan University Wuhan 430072 China Correspondence Ying Tang LU Tel 86 27 68752619 E mail yingtlu whu edu cn By screening tobacco cDNA library with MCK1 as a probe we isolated a cDNA clone NtCPK5 accession number AY971376 which encodes a typical calcium dependent protein kinase Sequence analyses indicated that NtCPK5 is related to both CPKs and CRKs superfamilies and has all of the three conserved domains of CPKs The biochemical activity of NtCPK5 was calcium dependent NtCPK5 had V max and K m of 526 nmol min mg and 210 µg ml respectively with calf thymus histone fraction III abbreviated to histone IIIs as substrate For substrate syntide 2 NtCPK5 showed a higher V max of 2008 nmol min mg and a lower K m of 30 µM The K 0 5 of calcium activation was 0 04 µM or 0 06 µM for histone IIIs or syntide 2 respectively The putative myristoylation and palmitoylation consensus sequence of NtCPK5 suggests that it could be a membrane anchoring protein Indeed our transient expression experiments with wild type and mutant forms of NtCPK5 GFP fusion proteins showed that NtCPK5 was localized to the plasma membrane

    Original URL path: http://www.cell-research.com/arts.asp?id=1151 (2016-02-14)
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  • Cell Research
    China 2 Graduate School of the Chinese Academy of Sciences Yuquan Road Shijingshan District Beijing 100039 China Correspondence Hong Qing LING Tel 86 10 64860377 E mail hqling genetics ac cn AtbHLH29 of Arabidopsis encoding a bHLH protein reveals a high similarity to the tomato FER which is proposed as a transcriptional regulator involved in controlling the iron deficiency responses and the iron uptake in tomato For identification of its biological functions AtbHLH29 was introduced into the genome of the tomato FER mutant T3238fer mediated by Agrobacterium tumefaciencs Transgenic plants were regenerated and the stable integration of AtbHLH29 into their genomes was confirmed by Southern hybridization Molecular analysis demonstrated that expression of the exogenous AtbHLH29 of Arabidopsis in roots of the FER mutant T3238fer enabled to complement the defect functions of FER The transgenic plants regained the ability to activate the whole iron deficiency responses and showed normal growth as the wild type under iron limiting stress Our transformation data demonstrate that AtbHLH29 is a functional ortholog of the tomato FER and can completely replace FER in controlling the effective iron acquisition in tomato Except of iron FER protein was directly or indirectly involved in manganese homeostasis due to that

    Original URL path: http://www.cell-research.com/arts.asp?id=1152 (2016-02-14)
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  • Cell Research
    Agricultural Science Beijing 100081 China Correspondence Jian Min WAN Tel 86 025 84396516 E mail wanjm njau edu cn Rice with low glutelin content is suitable as functional food for patients affected with diabetes and kidney failure The fine mapping of the gene s responsible for low glutelin content will provide information regarding the distribution of glutelin related genes in rice genome and will generate markers for the selection of low glutelin rice varieties Following an SDS PAGE screen of rice germplasm from Taihu Valley of China Japonica selection W3660 is identified to be a novel mutant characterized with low glutelin content For fine mapping the mutant gene for low glutelin content F 2 and F 3 populations were derived from a cross between W3660 and Jingrennuo SDS PAGE analysis of the total endosperm protein showed that the low glutelin content trait was controlled by a single dominant nuclear gene Genetic mapping using SSRs located this gene to chromosome 2 in the region between SSR2 001 SSR2 004 and RM1358 The distances of the two markers to the target gene were 1 1 cM and 3 8 cM respectively By semi quantitative RT PCR analysis the transcripts of GluB4 GluB5

    Original URL path: http://www.cell-research.com/arts.asp?id=1153 (2016-02-14)
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  • Cell Research
    Science and National Key Facility for Crop Gene Resource and Genetic Improvement Chinese Academy of Agricultural Sciences Beijing100081 China Correspondence Long MAO Tel 1 815 7537947 E mail lmao niu edu MicroRNAs miRNAs are 20 22 nucleotide non coding RNAs that play important roles in plant and animal development They are usually processed from larger precursors that can form stem loop structures Among 20 miRNA families that are conserved between Arabidopsis and rice the rice miR395 gene family was unique because it was organized into compact clusters that could be transcribed as one single transcript We show here that in fact this family had four clusters of total 24 genes Three of these clusters were segmental duplications They contained miR395 genes of both 120 bp and 66 bp long However only the latter was repeatedly duplicated The fourth cluster contained miR395 genes of two different sizes that could be the consequences of intergenic recombination of genes from the first three clusters On each cluster both 1 duplication and 2 duplication histories were observed based on the sequence similarity between miR395 genes some of which were nearly identical suggesting a recent origin This was supported by a miR395 locus survey among

    Original URL path: http://www.cell-research.com/arts.asp?id=1154 (2016-02-14)
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  • Cell Research
    of Science Technology Pingtung 91201 Taiwan Correspondence Fure Chyi CHEN E mail fchen mail npust edu tw Tissue culture has been widely used for mass propagation of Phalaenopsis However somaclonal variation occurred during micropropagation process posed a severe problem by affecting product quality In this study wild type and peloric flower buds of Phalaenopsis hybrids derived from flower stalk nodal culture were used for cDNA RAPD and cDNA suppression subtractive hybridization analyses in order to study their genetic difference in terms of expressed sequence tags A total of 209 ESTs from normal flower buds and 230 from mutants were sequenced These ESTs sequences can be grouped into several functional categories involved in different cellular processes including metabolism signal transduction transcription cell growth and division protein synthesis and protein localization and into a subcategory of proteins with unknown function Cymbidium mosaic virus transcript was surprisingly found expressed frequently in the peloric mutant of P Little Mary Real time RT PCR analysis on selected ESTs showed that in mutant flower buds a bZIP transcription factor TGA1a like protein was down regulated while up regulated genes include auxin regulated protein kinase cyclophilin and TCP like genes A retroelement clone was also preferentially expressed

    Original URL path: http://www.cell-research.com/arts.asp?id=1155 (2016-02-14)
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  • Cell Research
    1 2 Xu LIU 1 Ji Zeng JIA 1 1 Key Laboratory of Crop Germplasm Biotechnology Ministry of Agriculture Institute of Crop Sciences Chinese Academy of Agricultural Sciences Beijing 100081 China 2 Institute of Genetics and Physiology Hebei Academy of Agricultural and Forestry Sciences Shijiazhuang 050051 China Correspondence Ji Zeng JIA Tel 86 10 62186623 E mail jzjia mail caas net cn Wheat 1BL 1RS translocations are widely planted in China as well as in most of the wheat producing area in the world for their good qualities of disease resistance and high yield 1BL 1RS translocations are however poor in bread making partially caused by a family of small monomeric proteins w secalins which are encoded by genes on 1RS Based on published sequence of a rye w secalin gene we designed a pair of primers to cover the whole mature protein coding sequence A major band could be amplified from 1BL 1RS translocations but not from euploid wheat Using this primer set we conducted PCR amplification by using high fidelity Pfu polymerase on the genomic DNAs and cDNAs purified from a 1BL 1RS translocation Lankao 906 Sequencing analysis indicated that this gene family contains several members of 1150

    Original URL path: http://www.cell-research.com/arts.asp?id=1156 (2016-02-14)
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  • Cell Research
    ZHAO 3 Jiang Hua CHEN 1 Xiao He HU 1 Da LUO 1 2 1 National Laboratory of Plant Molecular Genetics Institute of Plant Physiology and Ecology Shanghai Institutes for Biological Sciences and Graduate School of the Chinese Academy of Sciences Chinese Academy of Sciences 300 Fenglin Road Shanghai 200032 China 2 School of Life Science and Biotechnology Shanghai Jiao Tong University 1954 Huashan Road Shanghai 200030 China 3 Department of Biochemistry School of Life Sciences Fudan University Shanghai 200433 China Correspondence Da LUO E mail dluo sibs ac cn Recent studies on leaf development demonstrate that the mechanism on the adaxial abaxial polarity pattern formation could be well conserved among the far related species in which PHANTASTICA PAHN like genes play important roles In this study we explored the conservation and diversity on functions of PHAN like genes during the compound leaf development in Lotus japonicus a papilionoid legume Two PHAN like genes in L japonicus LjPHANa and LjPHANb were found to originate from a gene duplication event and displayed different expression patterns during compound leaf development Two mutants reduced leaflets1 rel1 and reduced leaflets3 rel3 which exhibited decreased adaxial identity of leaflets and reduced leaflet initiation were identified

    Original URL path: http://www.cell-research.com/arts.asp?id=1157 (2016-02-14)
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  • Cell Research

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    Original URL path: /artsmore1.asp?id=95 (2016-02-14)




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