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  • Cell Research
    Universitaria Madrid 28040 Espania 2 Cancer Research UK Tumour Pathology Group Nuffield Department of Clinical Laboratory Sciences John Radcliffe Hospital Oxford OX3 9DU UK 3 Medical Informatics Unit Nuffield Department of Clinical Laboratory Sciences John Radcliffe Hospital Oxford OX3 9DU UK 4 Cancer Research UK Molecular Oncology Laboratory John Radcliffe Hospital Oxford OX3 9DU UK Correspondence Francesco Pezzella Tel 44 01865 220497 E mail francesco pezzella cancer org uk KDR kinase insert domain receptor phosphorylation induces several effects which lead eventually to cell proliferation and survival The precise mechanisms by which KDR once it is activated communicates with the nucleus are starting to be understood but have not yet been completely unravelled Two in vitro studies on animal cell lines reported in the literature have demonstrated that following stimulation with VEGF KDR is actually translocated within the nucleus Our aim was to investigate whether this translocation occurs in human cells both in vitro and in vivo Using laser scanning confocal microscopy a variable nuclear localization of phosphorylated and total KDR in cell lines and tumour samples was found In human neoplastic cell lines hypoxic stimulation greatly increased the nuclear amount of total KDR but less so that of the phosphorylated

    Original URL path: http://www.cell-research.com/arts.asp?id=1127 (2016-02-14)
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  • Cell Research
    vector based RNA interference and transgenesis Ming Li 1 Baerbel Rohrer 1 2 Departments of 1 Neurosciences and 2 Ophthalmology Division of Research Medical University of South Carolina 167 Ashley Ave SEI 511 Charleston SC 29425 USA Correspondence Baerbel Rohrer Tel 843 792 5086 E mail rohrer musc edu A vector based RNAi expression system was developed using the Xenopus tropicalis U6 promoter which transcribes small RNA genes by RNA polymerase III The system was first validated in a Xenopus laevis cell line designing a short hairpin DNA specific for the GFP gene Co transfection of the vector based RNAi and the GFP gene into Xenopus XR1 cells significantly decreased the number of GFP expressing cells and overall GFP fluorescence Vector based RNAi was subsequently validated in GFP transgenic Xenopus embryos Sperm nuclei from GFP transgenic males and RNAi con struct incubated sperm nuclei were used for fertilization respectively GFP mRNA and protein were reduced by 60 by RNAi in these transgenic embryos compared with the control This transgene driven RNAi is specific and stable in inhibiting GFP expression in the Xenopus laevis transgenic line Gene silencing by vector based RNAi and Xenopus transgenesis may provide an alternative for repression

    Original URL path: http://www.cell-research.com/arts.asp?id=1128 (2016-02-14)
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  • Cell Research
    Issue Top 10 VOLUME 16 ISSUE 1 1 2006 106 112 The identification of a new actin binding region in p57 Chang Zhen Liu 1 Yong Chen 1 Sen Fang Sui 1 1 Department of Biological Sciences and Biotechnology State Key Laboratory of Biomembranes Tsinghua University Beijing 100084 China Correspondence Sen Fang Sui Tel 86 10 62784768 E mail suisf mail tsinghua edu cn The actin binding protein p57 is a member of mammalian coronin like proteins The roles of this protein in phagocytic processes conceivably depend on its interactions with F actin Two regions p57 1 34 and p57 111 204 were previously reported to be actin binding sites In this study we found that the C terminal region of p57 p57 297 461 also possessed F actin binding activity Furthermore the leucine zipper domain at the C terminus of p57 297 461 was essential for this actin binding activity The F actin cross linking assay revealed that the region contained in p57 297 461 was sufficient to cross link actin filaments Our results strongly suggested that there was a new actin binding region at the C terminus of p57 Cell Research 2006 16 106 112 doi 10 1038

    Original URL path: http://www.cell-research.com/arts.asp?id=1129 (2016-02-14)
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  • Cell Research
    Avenue Newark NJ 07101 USA 3 Cancer Institute of New Jersey 195 Little Albany Street New Brunswick New Jersey 08903 2681 USA 4 PBL Biomedical Laboratories 131 Ethel Road West Suite 6 Piscataway NJ 08854 5900 USA Correspondence Sidney Pestka Tel 1 732 235 4567 E mail pestka umdnj edu The activation of Stat1 by the interferon gamma IFN γ receptor complex is responsible for the transcription of a significant portion of IFN γ induced genes Many of these genes are responsible for the induction of an apoptotic state in response to IFN γ In the absence of Stat1 activation IFN γ instead induces a proliferative response Modifying Stat1 activation by IFN γ may have pharmacological benefits We report that the rate of activation of Stat1 can be altered in HeLa cells by overexpressing either the IFN γR1 chain or the IFN γR2 chain These alterations occur in hematopoietic cell lines Raji cells and monocytic cell lines which have average and above average IFN γR2 surface expression activate Stat1 similarly to HeLa cells and HeLa cells overexpressing IFNγR2 respectively The rapid Stat1 activation seen in HeLa cells can be inhibited by overexpressing a chimeric IFN γR2 chain that does not

    Original URL path: http://www.cell-research.com/arts.asp?id=1130 (2016-02-14)
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  • Cell Research

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    Original URL path: /artsmore1.asp?id=91 (2016-02-14)


  • Cell Research
    infections autoimmune diseases and cancer Thus a better understanding of these signaling pathways is essential to our efforts in developing more effective regimes to prevent and treat infectious diseases as well as to combat autoimmune diseases and cancer In conjunction with the successful hosting of the 2005 Annual Meeting of the International Society for Interferon and Cytokine Research ISICR in Shanghai 10 20 10 24 2005 Cell Research is pleased to bring to our readers a special issue on Interferon Cytokine and Immunity This entire Special Issue includes 22 articles 17 reviews and 5 research articles contributed by a selected group of outstanding scientists in the field who had presented their work at the 2005 ISICR Annual Meeting We are grateful to their efforts in writing these excellent articles in a relatively short amount of time We would also like extend our special thanks to Professor Xin Yuan Liu chairperson of the 2005 ISICR Annual Meeting and Drs Sidney Pestka John A Cidlowski and Yufang Shi who have acted as Editors for this special issue and their efforts are instrumental to our successful assembly of an outstanding list of contributing authors We are also grateful to Dr Christopher D Krause

    Original URL path: http://www.cell-research.com/arts.asp?id=1100 (2016-02-14)
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  • Cell Research
    Molecular Genetics Microbiology and Immunology Robert Wood Johnson Medical School University of Medicine and Dentistry of New Jersey 661 Hoes Lane Piscataway New Jersey 08854 USA Correspondence Satish Devadas Tel 732 235 4502 E mail devadasa umdnj edu Granulocyte macrophage colony stimulating factor GM CSF is an important hematopoietic growth factor and immune modulator GM CSF also has profound effects on the functional activities of various circulating leukocytes It is produced by a variety of cell types including T cells macrophages endothelial cells and fibroblasts upon receiving immune stimuli Although GM CSF is produced locally it can act in a paracrine fashion to recruit circulating neutrophils monocytes and lymphocytes to enhance their functions in host defense Recent intensive investigations are centered on the application of GM CSF as an immune adjuvant for its ability to increase dendritic cell DC maturation and function as well as macrophage activity It is used clinically to treat neutropenia in cancer patients undergoing chemotherapy in AIDS patients during therapy and in patients after bone marrow transplantation Interestingly the hematopoietic system of GM CSF deficient mice appears to be normal the most significant changes are in some specific T cell responses Although molecular cloning of GM

    Original URL path: http://www.cell-research.com/arts.asp?id=1101 (2016-02-14)
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  • Cell Research
    Ozato Tel 301 496 9184 E mail ozatok nih gov Dendritic cells DC although a minor population in hematopoietic cells produce type I interferons IFN and other cytokines and are essential for innate immunity They are also potent antigen presenters and regulate adaptive immunity Among DC subtypes plasmacytoid DC pDC produce the highest amounts of type I IFN In addition pro and anti in flammatory cytokines such as IL 12 and IL 10 are induced in DC in response to Toll like receptor TLR signaling and upon viral infection Proteins in the IRF family control many aspects of DC activity IRF 8 and IRF 4 are essential for DC development They differentially control the development of four DC subsets IRF 8 mice are largely devoid of pDC and CD8a DC while IRF 4 mice lack CD4 DC IRF 8 IRF4 double knock out mice have only few CD8á CD4 DC that lack MHC II IRF proteins also control type I IFN induction in DC IRF 7 activated upon TLR signaling is required for IFN induction not only in pDC but also in conventional DC cDC and non DC cell types IRF 3 although contributes to IFN induction in fibroblasts is dispensable in IFN induction in DC Our recent evidence reveals that type I IFN induction in DC is critically dependent on IRF 8 which acts in the feedback phase of IFN gene induction in DC Type I IFN induction in pDC is mediated by MyD88 dependent signaling pathway and differs from pathways employed in other cells which mostly rely on TLR3 and RIG I family proteins Other pro inflammatory cytokines are produced in an IRF 5 dependent manner However IRF 5 is not required for IFN induction suggesting the presence of separate mechanisms for induction of type I IFN and

    Original URL path: http://www.cell-research.com/arts.asp?id=1102 (2016-02-14)
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