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  • Cell Research
    Center for Plant Molecular Genetics and AgroBiotechnology National Laboratory for Protein Engineering and Plant Genetic Engineering College of Life Sciences Peking University Beijing 100871 China 2 The National Plant Gene Research Center Beijing 100101 China Correspondence Hong Ya Gu Tel 86 10 6275 1847 E mail guhy pku edu cn Plasma membrane intrinsic proteins PIPs are a subfamily of aquaporins that enable fast and controlled translocation of water across the membrane In this study we systematically identified and cloned ten PIP genes from rice Based on the similarity of the amino acid sequences they encoded these rice PIP genes were classified into two groups and designated as OsPIP1 1 to OsPIP1 3 and OsPIP2 1 to OsPIP2 7 following the nomenclature of PIP genes in maize Quantitative RT PCR analysis identified three root specific and one leaf specific OsPIP genes Furthermore the expression profile of each OsPIP gene in response to salt drought and ABA treatment was examined in detail Analysis on transgenic plants over expressing of either OsPIP1 OsPIP1 1 or OsPIP2 OsPIP2 2 in wild type Arabidopsis showed enhanced tolerance to salt 100 mM of NaCl and drought 200 mM of mannitol but not to salt treatment of

    Original URL path: http://www.cell-research.com/arts.asp?id=1091 (2016-02-14)
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  • Cell Research
    Yuquan Road Shijingshan District Beijing 100039 China Correspondence Dao Wen Wang Tel 86 10 64889380 E mail dwwang genetics ac cn By reducing cysteine sulfinic acid in oxidized peroxiredoxin sulfiredoxin Srx plays an important role in oxidation stress resistance in yeast and human cells Here we report the first molecular and functional characterization of Srx homolog from higher plants Bioinformatic analysis revealed the presence of potential Srx encoding sequences in both monocot and dicot plant species Putative plant Srx proteins exhibited significant identities to their orthologs from yeast and human and contained the conserved signature sequence and residues essential for catalysis However unlike yeast and human orthologs plant Srxs were all predicted to possess chloroplast transit peptide in their primary structure The Srx proteins from Arabidopsis and rice designated as AtSrx and OsSrx respectively complemented functional defi ciency of Srx in the SRX1 deletion yeast cells A GFP fusion protein of AtSrx was targeted to chloroplast in Arabidopsis mesophyll protoplast AtSrx transcription occurred in both vegetative and reproductive organs and the highest transcript level was detected in leaves Under oxidation stress AtSrx transcript level was substantially increased which paralleled with enhanced transcription of 2 Cys peroxiredoxins that have been found

    Original URL path: http://www.cell-research.com/arts.asp?id=1092 (2016-02-14)
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  • Cell Research
    Start Tilman B 4000 Li間e Belgium 4 Biophysics Laboratory Division of Bioengineering National University of Singapore Singapore 117576 Singapore 5 Department of Biochemistry Shanghai Medical College of Fudan University Free Radical Regulation Research Center of Fudan University Shanghai 200032 China Correspondence Yuru Deng Tel 65 6874 1935 E mail phsdy nus edu sg Cardiogenic shock is the leading cause of death among patients hospitalized with acute myocardial infarction MI Understanding the mechanisms for acute pump failure is therefore important The aim of this study is to examine in an acute MI dog model whether mitochondrial bio energetic function within non ischemic wall regions are associated with pump failure Anterior MI was produced in dogs via ligation of left anterior descending LAD coronary artery that resulted in an infract size of about 30 of the left ventricular wall Measurements of hemodynamic status mitochondrial function free radical production and mitochondrial uncoupling protein 3 UCP3 expression were determined over 24 h period Hemodynamic measurements revealed a 50 reduction in cardiac output at 24 h post infarction when compared to baseline Biopsy samples were obtained from the posterior non ischemic wall during acute infarction ADP O ratios for isolated mitochondria from non ischemic myocardium at 6 h and 24 h were decreased when compared to the ADP O ratios within the same samples with and without palmitic acid PA GTP inhibition of PA stimulated state 4 respiration in isolated mitochondria from the non ischemic wall increased by 7 and 33 at 6 h and 24 h post infarction respectively when compared to sham and pre infarction samples This would suggest that the mitochondria are uncoupled and this is supported by an associated increase in UCP3 expression observed on western blots from these same biopsy samples Blood samples from the coronary sinus measured by electron

    Original URL path: http://www.cell-research.com/arts.asp?id=1093 (2016-02-14)
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  • Cell Research
    Milan Italy 2 ECVAM Institute for Health and Consumer Protection European Commission Joint Research Centre Ispra 21020 Italy 3 UCLA Department of Anesthesiology Los Angeles CA 90095 USA Correspondence Augusto Pessina Tel 39 2 50315072 E mail augusto pessina unimi it The very different effects of Cholera Toxin CT on cell growth and proliferation may depend on the type of ganglioside receptors in cell membranes and different signal transduction mechanisms triggered but other functions related to the drug resistance mechanisms can not be excluded The effect of CT treatment on the in vitro clonogenicity the Population Doubling Time PDT apoptosis PKA activation and Bax and Bcl 2 expression was evaluated in WEHI 3B cell line and its CT resistant subclone WEHI 3B CTRES In WEHI 3B parental cells the dramatic accumulation of cAMP induced by CT correlated well with PKA activation increased PDT value inhibition of clonogenicity and apoptosis H 89 treatment inhibited PKA activation by CT but did not protect the cells from apoptosis and growth inhibition In WEHI 3B CTRES no significant CT dependent accumulation of cAMP occurred with any increase of PKA activity and PDT In CT resistant cells WEHI 3B CTRES Bcl 2 expression was down regulated by both CT or drug treatment eg ciprofloxacin CPX although these cells were protected from CT dependent apoptosis but not from drug induced apoptosis Differently from other cell models described down regulation of Bcl 2 is proved to be independent on cAMP accumulation and PKA activation Our observations support the implication of cAMP dependent kinase PKA in the inhibition of WEHI 3B cells growth and suggest that in WEHI 3B CTRES Bcl 2 expression could be modulated by CT in the absence of cAMP accumulation Also in consideration of many contradictory data reported in literature our cell models of

    Original URL path: http://www.cell-research.com/arts.asp?id=1094 (2016-02-14)
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  • Cell Research
    of CD146 reduces the migration and proliferation of human endothelial cells Yanyong Kang 1 3 Fengcai Wang 1 2 Jing Feng 1 Dongling Yang 1 Xu Yang 3 Xiyun Yan 1 1 National Laboratory of Biomacromolecules Institute of Biophysics Chinese Academy of Science Beijing 100101 China 2 Chemistry Department Anyang teacher s College Anyang 455000 China 3 College of Life Science Central China Normal University Wuhan 430079 China Correspondence Xiyun Yan Tel 86 10 6488 8583 E mail yanxy sun5 ibp ac cn Our previous study has demonstrated that CD146 molecule is a biomarker on vascular endothelium which is involved in angiogenesis and tumor growth However the mechanism behind is not clear Here we have for the first time developed a novel CD146 blockade system using CD146 siRNA to study its function on endothelial cells Our data showed that CD146 siRNA specifically blocked the expression of CD146 on both mRNA and protein levels leading to the significant suppression of HUVEC proliferation adhesion and migration These results demonstrate that CD146 plays a key role in vascular endothelial cell activity and angiogenesis and CD146 siRNA can be used as a new inhibitor for anti angiogenesis therapy Cell Research 2006 16 313 318

    Original URL path: http://www.cell-research.com/arts.asp?id=1095 (2016-02-14)
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  • Cell Research
    sites in murine Nanog gene promoter Da Yong Wu 1 Zhen Yao 1 1 Laboratory of Molecular Cell Biology Laboratory of Stem Cell Biology Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences 320 Yue Yang Road Shanghai 200031 China Correspondence Da Yong Wu Tel 86 21 54921367 E mail dywu sibs ac cn Nanog gene plays a key role in maintaining pluripotency of ES cells and early embryonic cells A 5 flank sequence of the Nanog gene has been reported to be regulated differentially and two regulatory elements within the Nanog promoter namely Oct 4 and Sox 2 binding sites have been identified to regulate the transcriptional activity of Nanog gene In this report we identified the role of two putative Sp1 binding sites located in the Nanog gene 5 flanking region in regulation of murine Nanog gene transcription Mutation studies showed that the two sites were essential for the Nanog promoter activity Gel shift and supershift analysis showed that both sites specifically bind Sp1 and Sp3 Furthermore overexpression of dominant negative Sp1 or Sp3 mutants significantly inhibits Nanog promoter activity These results suggest that the transcription factor Sp1 and Sp3 are important

    Original URL path: http://www.cell-research.com/arts.asp?id=1096 (2016-02-14)
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  • Cell Research
    Shi 1 2 Li Yu 2 Yun xin Fu 3 Jing Fei Huang 1 Ke Qin Zhang 2 Ya ping Zhang 1 2 1 Laboratory of Cellular and Molecular Evolution and Molecular Biology of Domestic Animals Kunming Institute of Zoology Chinese Academy of Sciences Kunming 650223 China 2Laboratory for Conservation and Utilization of Bio resource Yunnan University Kunming 650091 China 3Human Genetics Center University of Texas at Houston Houston Texas 77030 USA Correspondence Ya ping Zhang Tel 86 871 5190761 E mail zhangyp1 263 net cn For developing efficient vaccines it is essential to identify which amino acid changes are most important to the survival of the virus We investigate the amino acid substitution features in the Avian Infectious Bronchitis Virus AIBV antigenic domain of a vaccine serotype DE072 and a virulent viral strain GA98 to better understand adaptive evolution of AIBV In addition the SARS Coronavirus SARS CoV was also analyzed in the same way It is interesting to find that extreme comparability exists between AIBV and SARS in amino acid substitution pattern It suggests that amino acid changes that result in overall shift of residue charge and polarity should be paid special attention to during the development of

    Original URL path: http://www.cell-research.com/arts.asp?id=1097 (2016-02-14)
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  • Cell Research

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    Original URL path: /artsmore1.asp?id=88 (2016-02-14)




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