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  • Cell Research
    Ferrari Universit degli Studi di Milano IRCCS Istituto Auxologico Italiano 20122 Milan Italy Dipartimento di Anatomia Patologica Ospedale San Paolo 20142 Milan Italy 5Ematologia 1 Centro Trapianti di Midollo Ospedale Maggiore IRCCS Universit degli Studi di Milano 20122 Milan Italy Correspondence Lorenza Lazzari Tel 39 02 5503 4053 E mail cbbank policlinico mi it The main goal of the study was to identify a novel source of human multipotent cells overcoming ethical issues involved in embryonic stem cell research and the limited availability of most adult stem cells Amniotic fluid cells AFCs are routinely obtained for prenatal diagnosis and can be expanded in vitro nevertheless current knowledge about their origin and properties is limited Twenty samples of AFCs were exposed in culture to adipogenic osteogenic neurogenic and myogenic media Differentiation was evaluated using immunocytochemistry RT PCR and Western blotting Before treatments AFCs showed heterogeneous morphologies They were negative for MyoD Myf 5 MRF4 Myogenin and Desmin but positive for osteocalcin PPARgamma2 GAP43 NSE Nestin MAP2 GFAP and beta tubulin III by RT PCR The cells expressed Oct 4 Rex 1 and Runx 1 which characterize the undifferentiated stem cell state By immunocytochemistry they expressed neural glial proteins mesenchymal and epithelial

    Original URL path: http://www.cell-research.com/arts.asp?id=1079 (2016-02-14)
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  • Cell Research
    University School of Medicine Japan Department of Life Science University of Tokyo 3 8 1 Komaba Meguro ku Tokyo 158 8902 Japan Department of Anatomy and Developmental Biology Tokyo Women抯 Medical College 8 1 Kawada cho Shinjuku ku Tokyo 162 8666 Japan Correspondence Takahiko Yokoyama Tel 81 75 251 5303 E mail tyoko koto kpu m ac jp The homozygous inv inversion of embryonic turning mouse mutant shows situs inversus and polycystic kidney disease both of which result from the lack of the inv gene Previously we suggested that inv may be important for the left杛ight axis formation not only in mice but also in Xenopus and that calmodulin regulates this inv protein function Here we isolated and characterized two Xenopus laevis homologs Xinv 1 and Xinv 2 of the mouse inv gene and performed functional analysis of the conserved IQ motifs that interact with calmodulin Xinv 1 expresses early in development in the same manner as mouse inv does Unexpectedly a full length Xenopus inv mRNA did not randomize cardiac orientation when injected into Xenopus embryos which is different from mouse inv mRNA Contrary to mouse inv mRNA Xenopus inv mRNA with mutated IQ randomized cardiac orientation The present

    Original URL path: http://www.cell-research.com/arts.asp?id=1080 (2016-02-14)
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