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  • Cell Research
    Shanghai Key Laboratory of Signaling and Disease Research Laboratory of Receptor based Bio medicine School of Life Sciences and Technology Tongji University Shanghai 200092 China 2 CAS Key Laboratory of Receptor Research the National Center for Drug Screening Shanghai Institute of Materia Medica Chinese Academy of Sciences Shanghai 201203 China 3 Institute of Neuroscience Key Laboratory of Primate Neurobiology CAS Center for Excellence in Brain Science Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai 200031 China Correspondence Xin Xie Tel 86 21 50801313 156 E mail xxie simm ac cn The direct conversion or transdifferentiation of non cardiac cells into cardiomyocytes by forced expression of transcription factors and microRNAs provides promising approaches for cardiac regeneration However genetic manipulations raise safety concerns and are thus not desirable in most clinical applications The discovery of full chemically induced pluripotent stem cells suggest the possibility of replacing transcription factors with chemical cocktails Here we report the generation of automatically beating cardiomyocyte like cells from mouse fibroblasts using only chemical cocktails These chemical induced cardiomyocyte like cells CiCMs express cardiomyocyte specific markers exhibit sarcomeric organization and possess typical cardiac calcium flux and electrophysiological features Genetic lineage tracing confirms the fibroblast origin of

    Original URL path: http://www.cell-research.com/arts.asp?id=2151 (2016-02-14)
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  • Cell Research
    and Sheng Cai Lin 1 1 State Key Laboratory of Cellular Stress Biology Innovation Center for Cell Signaling Network School of Life Sciences Xiamen University Xiamen Fujian 361005 China 2 Department of Urology Shanghai Jiao Tong University Affiliated Sixth People s Hospital Shanghai 200233 China 3 Department of Endocrinology and Metabolism Shanghai Jiao Tong University Affiliated Sixth People s Hospital Shanghai 200233 China 4 Department of Urology Zhongshan Hospital Xiamen University Xiamen Fujian 361004 China Correspondence Sheng Cai Lin Tel 86 592 218 2993 Fax 86 592 218 2993 E mail linsc xmu edu cn Hypoxia inducible factors HIFs are master regulators of adaptive responses to low oxygen and their α subunits are rapidly degraded through the ubiquitination dependent proteasomal pathway after hydroxylation Aberrant accumulation or activation of HIFs is closely linked to many types of cancer However how hydroxylation of HIFα and its delivery to the ubiquitination machinery are regulated remains unclear Here we show that Rho related BTB domain containing protein 3 RHOBTB3 directly interacts with the hydroxylase PHD2 to promote HIFα hydroxylation RHOBTB3 also directly interacts with the von Hippel Lindau VHL protein a component of the E3 ubiquitin ligase complex facilitating ubiquitination of HIFα Remarkably RHOBTB3

    Original URL path: http://www.cell-research.com/arts.asp?id=2152 (2016-02-14)
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  • Cell Research
    Department of Biology University of Fribourg Fribourg CH 1700 Switzerland 2 National Center for Protein Science Shanghai State Key Laboratory of Molecular Biology Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences 320 Yue Yang Road Shanghai 200031 China Correspondence Claudio De Virgilio E mail claudio devirgilio unifr ch Jianping Ding E mail jpding sibs ac cn The target of rapamycin complex 1 TORC1 integrates various hormonal and nutrient signals to regulate cell growth proliferation and differentiation Amino acid dependent activation of TORC1 is mediated via the yeast EGO complex EGOC consisting of Gtr1 Gtr2 Ego1 and Ego3 Here we identify the previously uncharacterized Ycr075w a Ego2 protein as an additional EGOC component that is required for the integrity and localization of the heterodimeric Gtr1 Gtr2 GTPases equivalent to mammalian Rag GTPases We also report the crystal structure of the Ego1 Ego2 Ego3 ternary complex EGO TC at 2 4 Å resolution in which Ego2 and Ego3 form a heterodimer flanked along one side by Ego1 Structural data also reveal the structural conservation of protein components between the yeast EGO TC and the human Ragulator which acts as a GEF for Rag GTPases Interestingly

    Original URL path: http://www.cell-research.com/arts.asp?id=2153 (2016-02-14)
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  • Cell Research
    Fan 1 Xianping Wang 1 Yongfang Zhao 1 and Xuejun C Zhang 1 1 National Laboratory of Macromolecules National Center of Protein Science Beijing Institute of Biophysics Chinese Academy of Sciences 15 Datun Road Beijing 100101 China 2 University of Chinese Academy of Sciences Beijing 100049 China 3 School of Life Sciences University of Science and Technology of China Hefei Anhui 230027 China 4 College of Biotechnology Tianjin University of Science and Technology 29 13th Street TEDA Tianjin 300457 China Correspondence Yongfang Zhao E mail yongfangzhao ibp ac cn Xuejun C Zhang E mail zhangc ibp ac cn Multidrug resistance is a serious threat to public health Proton motive force driven antiporters from the major facilitator superfamily MFS constitute a major group of multidrug resistance transporters Currently no reports on crystal structures of MFS antiporters in complex with their substrates exist The E coli MdfA transporter is a well studied model system for biochemical analyses of multidrug resistance MFS antiporters Here we report three crystal structures of MdfA ligand complexes at resolutions up to 2 0 Å all in the inward facing conformation The substrate binding site sits proximal to the conserved acidic residue D34 Our mutagenesis studies support the

    Original URL path: http://www.cell-research.com/arts.asp?id=2154 (2016-02-14)
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  • Cell Research
    Online Publication Current Issue Top 10 VOLUME 25 ISSUE 9 9 2015 1074 1077 Expansion of CRISPR Cas9 genome targeting sites in zebrafish by Csy4 based RNA processing Wei Qin 1 2 Fang Liang 1 Yan Feng 1 Haipeng Bai 1 Ruibin Yan 2 Song Li 1 2 and Shuo Lin 1 3 1 Laboratory of Chemical Genomics School of Chemical Biology and Biotechnology Peking University Shenzhen Graduate School Shenzhen 518055 China 2 Shenzhen Shengjie Biotech Co Ltd Shenzhen 518055 China 3 Department of Molecular Cell and Developmental Biology University of California Los Angeles CA 90095 USA Correspondence Shuo Lin Tel 1 310 2674 970 Fax 1 310 2674 971 E mail shuolin ucla edu With its advantages of simple design and cost efficiency the CRISPR Cas9 technology has been widely adapted for genome editing in different species including zebrafish1 In zebrafish studies guide RNA gRNA is usually produced via in vitro transcription followed by microinjection with Cas9 mRNA into embryos The vectors currently used for production of gRNA contain either a T7 or SP6 promoter in vitro or U6 promoter in vivo Among these T7 promoter is most popularly used due to its high efficiency and therefore limits the

    Original URL path: http://www.cell-research.com/arts.asp?id=2155 (2016-02-14)
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  • Cell Research
    Wenqing Xu 1 1 Department of Biological Structure University of Washington Seattle WA 98195 USA 2 State Key Laboratory of Biotherapy and Cancer Center West China Hospital Sichuan University and Collaborative Innovation Center for Biotherapy Chengdu 610041 China 3 Van Andel Research Institute Grand Rapids MI 49503 USA 4 VARI SIMM Center CAS Key Laboratory of Receptor Research Shanghai Institute of Materia Medica Chinese Academy of Sciences Shanghai 201203 China

    Original URL path: http://www.cell-research.com/arts.asp?id=2156 (2016-02-14)
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  • Cell Research
    Zhang 6 Chun Sheng 7 Hongyan Wang 2 and Ping Hu 1 1 State Key Laboratory of Cell Biology Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences 320 Yueyang Road Shanghai 200031 China 2 Key Laboratory of Systems Biology Innovation Center for Cell Signaling Network Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai 200031 China 3 CAS Key Laboratory of Computational Biology CAS MPG Partner Institute for Computational Biology 320 Yueyang Road Shanghai 200031 China 4 Department of Chemical Pathology Li Ka Shing Institute of Health Sciences The Chinese University of Hong Kong Hong Kong SAR China 5 Department of Orthopaedics and Traumatology Li Ka Shing Institute of Health Sciences The Chinese University of Hong Kong Hong Kong SAR China 6 School of Chinese Medicine The Chinese University of Hong Kong Hong Kong SAR China 7 Shanghai Normal University Guilin Road Shanghai 200234 China In the initial published version of this article there was an error in the labeling of Figure 2I Figure 2I displays representative images of RFP Pax7 Laminin DAPI staining of muscle tissues after transplantation of MuSCs cultured in either F10 medium or T cell conditional medium The top panel in Figure 2I was previously labeled as T cell medium P0 The correct labeling should be F10 medium P0 We performed transplantation experiments using MuSCs cultured in F10 medium T cell conditional medium or medium supplemented with IL1α IL13 IFNγ and TNFα in a side by side manner to allow direct comparison of the engraftment efficiency The same set of data was divided into two parts as shown in Figure 2I and Figure 5E to show the post transplantation localization of MuSCs that had been previously cultured in T cell medium and

    Original URL path: http://www.cell-research.com/arts.asp?id=2158 (2016-02-14)
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  • Cell Research

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    Original URL path: /artsmore1.asp?id=187 (2016-02-14)




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