archive-com.com » COM » C » CELL-RESEARCH.COM

Total: 1754

Choose link from "Titles, links and description words view":

Or switch to "Titles and links view".
  • Cell Research
    Shanghai Jiao Tong University School of Medicine Shanghai 200025 China 3 Key Laboratory of Cell Differentiation and Apoptosis of Chinese Ministry of Education Shanghai Jiao Tong University School of Medicine Shanghai 200025 China 4 Graduate School of Chinese Academy of Sciences Beijing 100000 China Correspondence Ying Jin Tel 86 21 63852591 E mail yjin sibs ac cn Transcription factor Oct4 plays critical roles in maintaining pluripotency and controlling lineage commitment of embryonic stem cells ESCs Our previous study indicates that Wwp2 a mouse HECT type E3 ubiquitin ligase ubiquitinates Oct4 and promotes its degradation in a heterologous system However roles of Wwp2 in regulating endogenous Oct4 protein levels as well as molecular characteristics of the function of Wwp2 have not been determined Here we report that Wwp2 plays an important role in Oct4 ubiquitination and degradation during differentiation of embryonal carcinoma cells ECCs although it does not appear to affect Oct4 protein levels in the undifferentiated ECCs and ESCs Importantly inhibition of Wwp2 expression by specific RNA interference elevates the Oct4 protein level leading to attenuation in retinoid acid induced activation of differentiation related marker genes Mechanistically Wwp2 catalyzes Oct4 poly ubiquitination via the lysine 63 linkage in a dosage

    Original URL path: http://www.cell-research.com/arts.asp?id=562 (2016-02-14)
    Open archived version from archive


  • Cell Research
    qd sd cn Iron plays a key role in Parkinson s disease PD Increased iron content of the substantia nigra SN has been found in PD patients and divalent metal transporter 1 DMT1 has been shown to be up regulated in the SN of both MPTP induced PD models and PD patients However the mechanisms underlying DMT1 up regulation are largely unknown In the present study we observed that in the SN of 6 hydroxydopamine 6 OHDA induced PD rats DMT1 with the iron responsive element IRE DMT1 IRE but not DMT1 without IRE DMT1 IRE was up regulated suggesting that increased DMT1 IRE expression might account for nigral iron accumulation in PD rats This possibility was further assessed in an in vitro study using 6 OHDA treated and DMT1 IRE over expressing MES23 5 cells In 6 OHDA treated MES23 5 cells increased iron regulatory protein IRP 1 and IRP2 expression was observed while silencing of IRPs dramatically diminished 6 OHDA induced DMT1 IRE up regulation Pretreatment with N acetyl L cysteine fully suppressed IRPs up regulation by inhibition of 6 OHDA induced oxidative stress Increased DMT1 IRE expression resulted in increased iron influx by MES23 5 cells Our

    Original URL path: http://www.cell-research.com/arts.asp?id=563 (2016-02-14)
    Open archived version from archive

  • Cell Research
    a histone chaperone required for repression of ribosomal RNA gene expression in Arabidopsis Hong Li and Sheng Luan Hong Li and Sheng Luan Correspondence Sheng Luan Tel 1 510 642 4995 E mail sluan berkeley edu Chromatin structure is important for controlling gene expression but mechanisms underlying chromatin remodeling are not fully understood Here we report that an FKBP FK506 binding protein type immunophilin AtFKBP53 possesses histone chaperone activity and is required for repressing ribosomal gene expression in Arabidopsis The AtFKBP53 protein is a multidomain FKBP with a typical peptidylprolyl isomerase PPIase domain and several highly charged domains Using nucleosome assembly assays we showed that AtFKBP53 has histone chaperone activity and the charged acidic domains are sufficient for the activity We show that AtFKBP53 interacts with histone H3 through the acidic domains whereas the PPIase domain is dispensable for histone chaperone activity or histone binding Ribosomal RNA gene 18S rDNA is overexpressed when AtFKBP53 activity is reduced or eliminated in Arabidopsis plants Chromatin immunoprecipitation assay showed that AtFKBP53 is associated with the 18S rDNA gene chromatin implicating that AtFKBP53 represses rRNA genes at the chromatin level This study identifies a new histone chaperone in plants that functions in chromatin remodeling

    Original URL path: http://www.cell-research.com/arts.asp?id=564 (2016-02-14)
    Open archived version from archive

  • Cell Research
    Center Pittsburgh PA 15213 USA 3 Xinyuan Institute of Medicine and Biotechnology Zhejiang Sci Tech University Hangzhou 310018 China Correspondence Xin Yuan Liu Lu Yuan Li Tel 86 21 54921126 86 21 54921126 E mail xyliu sibs ac cn lil upmc edu Vascular endothelial cell growth inhibitor VEGI is a member of the tumor necrosis factor superfamily and plays an important role in vascular homeostasis In this study to investigate the anticancer therapeutic potential of this gene a secreted isoform of VEGI VEGI 251 was inserted into a selectively replicating adenovirus with E1B 55 kDa gene deletion ZD55 to construct ZD55 VEGI 251 We report here that secreted VEGI 251 produced from ZD55 VEGI 251 infected cancer cells potently inhibits endothelial cell proliferation tube formation in vitro and angiogenesis of chick chorioallantoic membrane in vivo Additionally ZD55 VEGI 251 infection leads to a much more severe cytopathic effect than control viruses on several human cancer cell lines including cervical cancer cell line HeLa hepatoma cell line SMMC 7721 and colorectal cancer cell line SW620 Further study reveals that the increased cytotoxicity is a result of VEGI 251 autocrine dependent mitochondria mediated apoptosis accompanied by caspase 9 activation enhanced caspase 3

    Original URL path: http://www.cell-research.com/arts.asp?id=565 (2016-02-14)
    Open archived version from archive

  • Cell Research
    20 ISSUE 3 3 2010 379 382 Targeted conditional gene knockout in human embryonic stem cells Lei Bu Xiaolin Gao Xin Jiang Kenneth R Chien and Zhong Wang Cardiovascular Research Center Massachusetts General Hospital Harvard Medical School and Harvard Stem Cell Institute Richard Simches Research Center 185 Cambridge Street Boston MA 02114 USA Correspondence Kenneth R Chien Zhong Wang Tel 617 643 3440 617 643 3444 E mail kchien partners

    Original URL path: http://www.cell-research.com/arts.asp?id=566 (2016-02-14)
    Open archived version from archive

  • Cell Research
    Tang Hai 1 Jie Hao 1 2 Chang long Guo 1 2 Xiang Wang 3 Liu Wang 1 Fanyi Zen 3 4 and Qi Zhou 1 1 State Key Laboratory of Reproductive Biology Institute of Zoology Chinese Academy of Sciences Beijing 100101 China 2 Graduate School of Chinese Academy of Sciences Beijing 100049 China 3 Shanghai Institute of Medical Genetics Shanghai Children s Hospital Shanghai Jiao Tong University School of

    Original URL path: http://www.cell-research.com/arts.asp?id=567 (2016-02-14)
    Open archived version from archive

  • Cell Research
    an H3K4 demethylase regulating flowering time in Arabidopsis Falong Lu 1 2 Xia Cui 1 Shuaibin Zhang 1 2 Chunyan Liu 1 and Xiaofeng Cao 1 1 National Key Laboratory of Plant Genomics and Center for Plant Gene Research Institute of Genetics and Developmental Biology Chinese Academy of Sciences No 1 Bei Chen West Road Chaoyang District Beijing 100101 China 2 Graduate School Chinese Academy of Sciences Beijing 100049 China

    Original URL path: http://www.cell-research.com/arts.asp?id=568 (2016-02-14)
    Open archived version from archive

  • Cell Research

    (No additional info available in detailed archive for this subpage)
    Original URL path: /artsmore1.asp?id=39 (2016-02-14)




  •