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  • Cell Research
    Free Sample Issue Submission Advanced Online Publication Current Issue Top 10 VOLUME 20 ISSUE 8 8 2010 869 871 A clear link between endogenous retroviral LTR activity and Hodgkin s lymphoma Katryn J Stacey and Vitaliya Sagulenko The University of Queensland School of Chemistry and Molecular Biosciences Brisbane Qld 4072 Australia Correspondence Katryn J Stacey E mail katryn stacey uq edu au Cell Research 2010 20 869 871 doi 10

    Original URL path: http://www.cell-research.com/arts.asp?id=485 (2016-02-14)
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  • Cell Research
    4 Huirong Yang 2 3 Hanqing Lin 6 Yiqin Wang 6 Charlie Degui Chen 6 and Yanhui Xu 1 2 3 1 Cancer Institute Shanghai Cancer Center Fudan University Department of Oncology Shanghai Medical College Fudan University shanghai 200032 China 2 School of Life Sciences Fudan University 220 Han Dan Road shanghai 200433 China 3 Institutes of Biomedical Sciences Fudan University 130 Dong An Road shanghai 200032 China 4 Beijing Synchrotron Radiation Facility Institute of High Energy Physics Chinese Academy of Sciences Beijing 100049 China 5 School of Pharmacy Fudan University South Chemistry Building Room 315 826 Zhangheng Road shanghai 201203 China 6 State Key Laboratory of Molecular Biology Shanghai Key laboratory of Molecular Andrology Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences 320 Yueyang Road shanghai 200031 China Correspondence Charlie Degui Chen Yanhui Xu E mail cdchen sibs ac cn xuyh fudan edu cn Histone lysine methylation can be removed by JmjC domain containing proteins in a sequence and methylation state specific manner However how substrate specificity is determined and how the enzymes are regulated were largely unknown We recently found that ceKDM7A a PHD and JmjC domain containing protein is a histone demethylase specific for H3K9me2 and H3K27me2 and the PHD finger binding to H3K4me3 guides the demethylation activity in vivo To provide structural insight into the molecular mechanisms for the enzymatic activity and the function of the PHD finger we solved six crystal structures of the enzyme in apo form and in complex with single or two peptides containing various combinations of H3K4me3 H3K9me2 and H3K27me2 modifications The structures indicate that H3K9me2 and H3K27me2 interact with ceKDM7A in a similar fashion and that the peptide binding specificity is determined by a network of specific interactions The geometrical measurement of the

    Original URL path: http://www.cell-research.com/arts.asp?id=487 (2016-02-14)
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  • Cell Research
    4 Yi Liu 3 4 Yanhui Xu 3 4 and Charlie Degui Chen 1 1 State Key Laboratory of Molecular Biology Shanghai Key Laboratory of Molecular Andrology Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences 320 Yueyang Road Shanghai 200031 China 2 Affiliated Hospital of Nantong University Nantong 226001 China 3 School of Life Sciences Fudan University 220 Han Dan Road Shanghai 200433 China 4 Institutes of Biomedical Sciences Fudan University 130 Dong An Road Shanghai 200032 China Correspondence Charlie Degui Chen Yanhui Xu E mail cdchen sibs ac cn xuyh fudan edu cn H3K9me2 and H3K27me2 are important epigenetic marks associated with transcription repression while H3K4me3 is associated with transcription activation It has been shown that active and repressive histone methylations distribute in a mutually exclusive manner but the underlying mechanism was poorly understood Here we identified ceKDM7A a PHD plant homeodomain and JmjC domain containing protein as a histone demethylase specific for H3K9me2 and H3K27me2 We further demonstrated that the PHD domain of ceKDM7A bound H3K4me3 and H3K4me3 co localized with ceKDM7A at the genome wide level Disruption of the PHD domain binding to H3K4me3 reduced the demethylase activity in vivo

    Original URL path: http://www.cell-research.com/arts.asp?id=488 (2016-02-14)
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  • Cell Research
    Sciences East China Normal University 500 Dongchuan Road Shanghai 200241 China 2 Department of Medicine Baylor College of Medicine Houston TX 77030 USA Correspondence Jiemin Wong Shuo Dong E mail jmweng bio ecnu edu cn sdong bcm edu Recent studies have identified mutations in PHF8 an X linked gene encoding a JmjC domain containing protein as a causal factor for X linked mental retardation XLMR and cleft lip cleft palate However the underlying mechanism is unknown Here we show that PHF8 is a histone demethylase and coactivator for retinoic acid receptor RAR Although activities for both H3K4me3 2 1 and H3K9me2 1 demethylation were detected in cellular based assays recombinant PHF8 exhibited only H3K9me2 1 demethylase activity in vitro suggesting that PHF8 is an H3K9me2 1 demethylase whose specificity may be modulated in vivo Importantly a mutant PHF8 phenylalanine at position 279 to serine identified in the XLMR patients is defective in enzymatic activity indicating that the loss of histone demethylase activity is causally linked with the onset of disease In addition we show that PHF8 binds specifically to H3K4me3 2 peptides via an N terminal PHD finger domain Consistent with a role for PHF8 in neuronal differentiation knockdown of

    Original URL path: http://www.cell-research.com/arts.asp?id=489 (2016-02-14)
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  • Cell Research
    Kim 1 1 Department of Biochemistry and Molecular Biology BK21 project Medical Research Center for Bioreaction to Reactive Oxygen Species and Biomedical Science Institute School of Medicine Kyung Hee University Seoul 130 701 Korea 2 Division of Radiation Effect Korea Institute of Radiological and Medical Sciences Seoul 139 706 Korea 3 Department of Urology College of Medicine Cheju National University Cheju 690 756 Korea 4 Department of Urology School of Medicine Kyung Hee University Seoul 130 701 Korea 5 Cardiovascular Center East West Neomedical Center Kyung Hee University Seoul 134 090 Korea Correspondence Sung Soo Kim Tel 82 2 961 0524 E mail sgskim khu ac kr Oncogenic H Ras G12V and its variants have been shown to inhibit muscle differentiation However the role of proto oncogenic Ras c Ras in muscle differentiation remains unclear The active GTP bound form of Ras has been known to associate with diverse effectors including Raf phosphatidylinositol 3 kinase PI3K Ral GDS and other molecules to transmit downstream signals We hypothesize that c Ras may stimulate muscle differentiation by selectively activating PI3K an important mediator for muscle differentiation In our experiments inhibition of c Ras by farnesyltransferase inhibitors and a dominant negative form of H Ras Ras S17N suppressed muscle differentiation Consistently individual knockdown of H Ras K Ras and N Ras by siRNAs all blocked muscle differentiation Interestingly we found that c Ras preferentially interacts with PI3K rather than its major binding partner c Raf during myogenic differentiation with total c Ras activity remaining unchanged PI3K and its downstream myogenic pathway the Nox2 NF κB inducible nitric oxide synthase iNOS pathway were found to be suppressed by inhibition of c Ras activity during differentiation Furthermore expression of a constitutively active form of PI3K completely rescued the differentiation block and reactivated the Nox2 NF

    Original URL path: http://www.cell-research.com/arts.asp?id=490 (2016-02-14)
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  • Cell Research
    Long Biao Guo 2 Qian Qian 2 and Hong Wei Xue 1 1 National Key Laboratory of Plant Molecular Genetics Institute of Plant Physiology and Ecology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai 200032 China 2 State Key Laboratory of Rice Biology China National Rice Research Institute Hangzhou 310006 Zhejiang China Correspondence Hong Wei Xue Qian Qian E mail hwxue sibs ac cn qianqian188 hotmail com As an important agronomic trait inclination of leaves is crucial for crop architecture and grain yields To understand the molecular mechanism controlling rice leaf angles one rice leaf inclination2 lc2 three alleles mutant was identified and functionally characterized Compared to wild type plants lc2 mutants have enlarged leaf angles due to increased cell division in the adaxial epidermis of lamina joint The LC2 gene was isolated through positional cloning and encodes a vernalization insensitive 3 like protein Complementary expression of LC2 reversed the enlarged leaf angles of lc2 plants confirming its role in controlling leaf inclination LC2 is mainly expressed in the lamina joint during leaf development and particularly is induced by the phytohormones abscisic acid gibberellic acid auxin and brassinosteroids LC2 is localized in the nucleus and defects of LC2

    Original URL path: http://www.cell-research.com/arts.asp?id=491 (2016-02-14)
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  • Cell Research
    Peking University Beijing 100871 China Correspondence Chuanmao Zhang Tel 86 10 62757173 E mail zhangcm pku edu cn Pericentrin a conserved centrosomal component provides the structural scaffold to anchor numerous centrosomal proteins and thus plays an essential role in the organization and function of the centrosome and the mitotic spindle Although pericentrin was shown to localize in the cytoplasm and reported to be sensitive to leptomycin B LMB a specific inhibitor of Crm1 the regions within pericentrin that serve as signals for transporting in and out of the nucleus have not yet been identified In this study we identified five novel nuclear export signals NESs in pericentrin with diverse export activities All of the five NESs could bind to Crm1 in a LMB sensitive way when mediating the nuclear export of pericentrin We also demonstrated that the region of amino acids 8 42 in pericentrin contains a tripartite nuclear localization signal NLS consisting of three clusters of basic amino acids The NLS of pericentrin binds to importin β directly or via the adaptor importin α to form the import complex which could be disrupted by RanQ69L a dominant negative Ran GTPase possessing high affinity for importin β Furthermore we found

    Original URL path: http://www.cell-research.com/arts.asp?id=492 (2016-02-14)
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  • Cell Research
    Liu 1 Tian Xu Han 2 Li Lin Du 2 and Jin Qiu Zhou 1 1 The State Key Laboratory of Molecular Biology Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Graduate School of the Chinese Academy of Sciences 320 Yue Yang Road Shanghai 200031 China 2 National Institute of Biological Sciences Beijing 7 Science Park Road Zhong Guan Cun Life Science Park

    Original URL path: http://www.cell-research.com/arts.asp?id=493 (2016-02-14)
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