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  • Cell Research
    200031 China Correspondence Jianping Ding Tel 86 21 5492 1619 E mail jpding sibs ac cn Human cytosolic NADP IDH IDH1 has recently been found to be involved in tumorigenesis Notably the tumor derived IDH1 mutations identified so far mainly occur at Arg132 and mutation R132H is the most prevalent one This mutation impairs the oxidative IDH activity of the enzyme but renders a new reduction function of converting α ketoglutarate αKG to 2 hydroxyglutarate Here we report the structures of the R132H mutant IDH1 with and without isocitrate ICT bound The structural data together with mutagenesis and biochemical data reveal a previously undefined initial ICT binding state and demonstrate that IDH activity requires a conformational change to a closed pre transition state Arg132 plays multiple functional roles in the catalytic reaction in particular the R132H mutation hinders the conformational changes from the initial ICT binding state to the pre transition state leading to the impairment of the IDH activity Our results describe for the first time that there is an intermediate conformation that corresponds to an initial ICT binding state and that the R132H mutation can trap the enzyme in this conformation therefore shedding light on the molecular mechanism

    Original URL path: http://www.cell-research.com/arts.asp?id=449 (2016-02-14)
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  • Cell Research
    2 Mutsuko Ouchi 3 Toru Ouchi 3 and Chu Xia Deng 1 1 Genetics of Development and Disease Branch 10 9N105 National Institute of Diabetes Digestive and Kidney Diseases National Institutes of Health Bethesda MD 20892 USA 2 Department of Gynecologic and Breast Pathology Armed Forces Institute of Pathology and American Registry of Pathology Washington DC 20306 USA 3 Department of Medicine NUHS Pritzker School of Medicine The University of Chicago Evanston IL 60201 USA 4 Present address Department of Gastroenterology Hepatology and Nutrition Unit 1466 MD Anderson Cancer Center University of Texas 1400 Pressler Street Houston TX 77030 3722 USA 5 present address INSERM UMR S 747 Unite de Pharmacologie Toxicologie et Signalisation Cellulaire Centre Universitaire des Saints P鑢es 45 rue des Saints Peres 75006 Paris France Correspondence Chu Xia Deng Tel 1 301 402 7225 E mail chuxiad bdg10 niddk nih gov Global DNA hypomethylation at CpG islands coupled with local hypermethylation is a hallmark for breast cancer yet the mechanism underlying this change remains elusive In this study we showed that DNMT1 which encodes a methylation maintenance enzyme is a transcriptional target of BRCA1 BRCA1 binds to the promoter of the DNMT1 gene through a potential OCT1 site and the binding is required for maintaining a transcriptional active configuration of the promoter in both mouse and human cells We further demonstrated that impaired function of BRCA1 leads to global DNA hypomethylation loss of genomic imprinting and an open chromatin configuration in several types of tissues examined in a BRCA1 mutant mouse model at premaligant stages BRCA1 deficiency is also associated with significantly increased expression levels of several protooncogenes including c Fos Ha Ras and c Myc with a higher expression in tumors while premalignant mammary epithelial cells displayed an intermediate state between tumors and controls In human

    Original URL path: http://www.cell-research.com/arts.asp?id=450 (2016-02-14)
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  • Cell Research
    Qiang Wang 2 3 Wei Zuo 2 Xiaohong Fang 1 and Ye Guang Chen 2 1 Beijing National Laboratory for Molecular Sciences Institute of Chemistry Key Laboratory of Molecular Nanostructures and Nanotechnology Chinese Academy of Sciences Beijing 100190 China 2 State Key Laboratory of Biomembrane and Membrane Biotechnology School of Life Sciences Tsinghua University Beijing 100084 China 3 Current address Institute of Zoology Chinese Academy of Sciences Beijing 100101 China Correspondence Xiaohong Fang Ye Guang Chen E mail xfang iccas ac cn ygchen mail tsinghua edu cn Transforming growth factor β TGF β binds with two transmembrane serine threonine kinase receptors type II TβRII and type I receptors TβRI and one accessory receptor type III receptor TβRIII to transduce signals across cell membranes Previous biochemical studies suggested that TβRI and TβRIII are preexisted homo dimers Using single molecule microscopy to image green fluorescent protein labeled membrane proteins for the first time we have demonstrated that TβRI and TβRIII could exist as monomers at a low expression level Upon TGF β1 stimulation TβRI follows the general ligand induced receptor dimerization model for activation but this process is TβRII dependent The monomeric status of the non kinase receptor TβRIII is unchanged in

    Original URL path: http://www.cell-research.com/arts.asp?id=451 (2016-02-14)
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  • Cell Research
    1 1 Institute of Pathology University of Regensburg Franz Josef Strauss Allee 11 D 93053 Regensburg Germany Correspondence Anja Katrin Bosserhoff Tel 49 941 944 6705 E mail anja bosserhoff klinik uni regensburg de Malignant melanoma characterized by invasive local growth and early formation of metastases is the most aggressive type of skin cancer Melanoma inhibitory activity MIA secreted by malignant melanoma cells interacts with the cell adhesion receptors integrins α 4 β 1 and α 5 β 1 facilitating cell detachment and promoting formation of metastases In the present study we demonstrate that MIA secretion is confined to the rear end of migrating cells while in non migrating cells MIA accumulates in the actin cortex MIA protein takes a conventional secretory pathway including coat protein complex I COPI and coat protein complex II COPII dependent protein transport to the cell periphery where its final release depends on intracellular Ca 2 ions Interestingly the Ca 2 activated K channel subfamily N member 4 KCa3 1 known to be active at the rear end of migrating cells was found to support MIA secretion Secretion was diminished by the specific KCa3 1 channel inhibitor TRAM 34 and by expression of dominant negative

    Original URL path: http://www.cell-research.com/arts.asp?id=452 (2016-02-14)
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  • Cell Research
    and Joel LeMaoult 2 3 1 Department of Biochemistry University Clinic of Navarra Pamplona Spain 2 CEA I2BM Service de Recherches en Hemato Immunologie Paris F 75475 France 3 UMR E Universit Paris 7 IUH Hopital Saint Louis Paris France Correspondence Joel LeMaoult Tel 33 1 57 27 68 02 E mail Joel LeMaoult cea fr Trogocytosis is a rapid transfer between cells of membranes and associated proteins Trogocytic exchanges have been investigated between different cell types mainly in two cell systems involving one donor and one acceptor cell type Here we studied trogocytosis in a more complex system involving not only several immune cell subsets but also multiple tumor cells We show that CD4 T cells CD8 T cells and monocytes can acquire membrane patches and the intact proteins they contain from different tumor cells by multiple simultaneous trogocytoses The trogocytic capabilities of CD4 and CD8 T cells were found to be similar but inferior to that of autologous monocytes Activated peripheral blood mononuclear cells PBMCs may also exchange membranes between themselves in an all autologous system For this reason monocytes are capable of acquiring membranes from multiple tumor cell sources and transfer them again to autologous T cells

    Original URL path: http://www.cell-research.com/arts.asp?id=453 (2016-02-14)
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  • Cell Research
    Kun Wen 1 Sui Bing Miao 1 Zhenhua Jia 2 Hai Juan Hu 1 Rong Hua Sun 1 Yiling Wu 2 and Mei Han 1 1 Department of Biochemistry and Molecular Biology Institute of Basic Medicine Key Laboratory of Neural and Vascular Biology China Ministry of Education No 361 Zhongshan East Road Shijiazhuang 050017 China 2 Integration of Traditional and Western Medical Research Academy of Hebei Province No 238 Tianshan Street Hebei Medical University Shijiazhuang 050017 China Correspondence Mei Han Yiling Wu Tel 86 0311 86265563 86 0311 85901553 E mail hanmei hebmu edu cn jiatcm 163 com The increased proliferation and migration of vascular smooth muscle cells VSMCs are key events in the development of atherosclerotic lesions Baicalin an herb derived flavonoid compound has been previously shown to induce apoptosis and growth inhibition in cancer cells through multiple pathways However the potential role of baicalin in regulation of VSMC proliferation and prevention of cardiovascular diseases remains unexplored In this study we show that pretreatment with baicalin has a dose dependent inhibitory effect on PDGF BB stimulated VSMC proliferation accompanied with the reduction of proliferating cell nuclear antigen PCNA expression We also show that baicalin induced growth inhibition is associated with a decrease in cyclin E CDK2 activation and increase in p27 level in PDGF stimulated VSMCs which appears to be at least partly mediated by blockade of PDGF receptor β PDGFRβ extracellular signal regulated kinase 1 2 ERK1 2 signaling In addition baicalin was also found to inhibit adhesion molecule expression and cell migration induced by PDGF BB in VSMCs Furthermore using an animal carotid arterial balloon injury model we found that baicalin significantly inhibited neointimal hyperplasia Taken together our results reveal a novel function of baicalin in inducing growth arrest of PDGF stimulated VSMCs and suppressing neointimal hyperplasia

    Original URL path: http://www.cell-research.com/arts.asp?id=454 (2016-02-14)
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  • Cell Research
    Biology Ernest Mario School of Pharmacy Rutgers University Piscataway NJ 08854 USA 2 Department of Oral Biology New Jersey Dental School University of Medicine Dentistry of New Jersey Newark NJ 07101 USA 3 Current address Aab Cardiovascular Research Institute University of Rochester Medical Center Rochester NY 14642 USA Correspondence Yi Zhang Tel 1 919 843 8225 E mail yi zhang med unc edu Ephrin ligands interact with Eph receptors to regulate a wide variety of biological and pathological processes Recent studies have identified several downstream pathways that mediate the functions of these receptors Activation of the receptors by ephrin binding results in the phosphorylation of the receptor tyrosine residues These phosphorylated residues serve as docking sites for many of the downstream signaling pathways However the relative contributions of different phosphotyrosine residues remain undefined In the present study we mutated each individual tyrosine residues in the cytoplasmic domain of EphA3 receptor and studied the effects using cell migration process retraction and growth cone collapse assays Stimulation of the EphA3 receptor with ephrin A5 inhibits 293A cell migration reduces NG108 15 cell neurite outgrowth and induces growth cone collapse in hippocampal neurons Mutation of either Y602 or Y779 alone partially decreases EphA3

    Original URL path: http://www.cell-research.com/arts.asp?id=455 (2016-02-14)
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  • Cell Research
    1 Zili Zhang 2 Yixue Li 1 Xin Guang Zhu 3 and Qi Liu 1 1 School of Life Sciences and Biotechnology Shanghai Jiao Tong University Shanghai 200240 China 2 Faculty of Computer and Information Science Southwest University Chongqing 400715 China 3 CAS MPG Partner Institute for Computational Biology Shanghai Institutes for Biological Sciences Chinese Academy Sciences Shanghai 200031 China Correspondence Yixue Li Xin Guang Zhu Qi Liu Tel 86

    Original URL path: http://www.cell-research.com/arts.asp?id=456 (2016-02-14)
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