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  • Cell Research
    Submission Advanced Online Publication Current Issue Top 10 VOLUME 21 ISSUE 8 8 2011 1155 1156 DNA methylation a new twist in the tail Gavin Kelsey 1 2 1 Epigenetics Programme The Babraham Institute Cambridge CB22 3AT United Kingdom 2 Centre for Trophoblast Research University of Cambridge Cambridge CB2 3EG United Kingdom Correspondence Gavin Kelsey Tel 01223 496332 E mail gavin kelsey babraham ac uk Cell Research 2011 21 1155

    Original URL path: http://www.cell-research.com/arts.asp?id=309 (2016-02-14)
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  • Cell Research
    in dyskeratosis congenita the long and the short of iPS Suneet Agarwal and George Q Daley Division of Hematology Oncology Children s Hospital Boston Pediatric Oncology Dana Farber Cancer Institute Manton Center for Orphan Disease Research Harvard Stem Cell Institute Division of Hematology Brigham and Women s Hospital Department of Biological Chemistry and Molecular Pharmacology Harvard Medical School Howard Hughes Medical Institute Children s Hospital Boston 1 Blackfan Circle Boston

    Original URL path: http://www.cell-research.com/arts.asp?id=310 (2016-02-14)
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  • Cell Research
    Thomson Reuters 2013 Free Sample Issue Submission Advanced Online Publication Current Issue Top 10 VOLUME 21 ISSUE 8 8 2011 1161 1163 To go or not to go the Daisuke Aki and Yun Cai Liu Division of Cell Biology La Jolla Institute for Allergy and Immunology La Jolla CA 92037 USA Correspondence Yun Cai Liu E mail yuncail liai org Cell Research 2011 21 1161 1163 doi 10 1038 cr

    Original URL path: http://www.cell-research.com/arts.asp?id=311 (2016-02-14)
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  • Cell Research
    Genetics Graduate School of Medicine Kyoto University Kyoto 606 8501 Japan 4 CREST JST Japan Correspondence Jiyoung Lee Tel 81 3 5803 4864 E mail jlee gcoe tmd ac jp Germline stem GS cells were established from gonocytes and spermatogonia of postnatal mouse testes GS cells proliferate in the presence of several kinds of cytokines and a small percentage of GS cells also show spermatogonial stem cell SSC activity i e they differentiate into sperm after being transplanted into infertile mouse testes without endogenous spermatogenesis Interestingly in GS cell culture we also found that pluripotent stem cells multipotent germline stem cells mGS cells could be derived and these mGS cells do not have normal androgenetic genomic imprinting marks that are shown in GS cells e g H19 hypermethylation A new culture system for fetal male germ cells embryonic GS eGS cells has also been recently developed Although these cells exhibited SSC potential the offspring from cultured cells showed heritable imprinting defects in their DNA methylation patterns In an attempt to understand the self renewal machinery in SSCs we transfected H Ras and cylin D2 into GS cells and successfully reconstructed the SSC self renewal ability without using exogenous cytokines Although

    Original URL path: http://www.cell-research.com/arts.asp?id=312 (2016-02-14)
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  • Cell Research
    Shanghai 200031 China 2 The Graduate School Chinese Academy of Sciences 320 Yueyang Road Shanghai 200031 China 3 Biochemistry Laboratory School of Engineering and Science Jacobs University Bremen Campus Ring 1 Bremen 28759 Germany 4 Institute of Biophysics Chinese Academy of Sciences 15 Datun Road Beijing 100101 China 5 Novartis Institute of BioMedical Research 898 Halei Road Shanghai 201203 China Correspondence Guo Liang Xu Tel 86 21 5492 1332 E mail glxu sibs ac cn Cytosine methylation of genomic DNA controls gene expression and maintains genome stability How a specific DNA sequence is targeted for methylation by a methyltransferase is largely unknown Here we show that histone H3 tails lacking lysine 4 K4 methylation function as an allosteric activator for methyltransferase Dnmt3a by binding to its plant homeodomain PHD In vitro histone H3 peptides stimulated the methylation activity of Dnmt3a up to 8 fold in a manner reversely correlated with the level of K4 methylation The biological significance of allosteric regulation was manifested by molecular modeling and identification of key residues in both the PHD and the catalytic domain of Dnmt3a whose mutations impaired the stimulation of methylation activity by H3 peptides but not the binding of H3 peptides Significantly

    Original URL path: http://www.cell-research.com/arts.asp?id=313 (2016-02-14)
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  • Cell Research
    Chinese Academy of Medical Sciences Peking Union Medical College 5 Dong Dan San Tiao Beijing 100005 China Correspondence Depei Liu Tel 86 10 65296415 E mail liudp pumc edu cn A wide variety of nuclear regulators and enzymes are subjected to acetylation of the lysine residue which regulates different aspects of protein functions The MYST family histone acetyltransferase human ortholog of MOF hMOF plays critical roles in transcription activation by acetylating nucleosomal H4K16 In this study we found that hMOF acetylates itself in vitro and in vivo and the acetylation is restricted to the conserved MYST domain C2HC zinc finger and HAT of which the K274 residue is the major autoacetylation site Furthermore the class III histone deacetylase SIRT1 was found to interact with the MYST domain of hMOF through the deacetylase catalytic region and deacetylate autoacetylated hMOF In vitro binding assays showed that non acetylated hMOF robustly binds to nucleosomes while acetylation decreases the binding ability In HeLa cells the recruitment of hMOF to the chromatin increases in response to SIRT1 overexpression and decreases after knockdown of SIRT1 The acetylation mimic mutation K274Q apparently decreases the chromatin recruitment of hMOF as well as the global H4K16Ac level in HeLa

    Original URL path: http://www.cell-research.com/arts.asp?id=314 (2016-02-14)
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  • Cell Research
    Union Medical College 5 Dong Dan San Tiao Beijing 100005 China Correspondence Jun Wu Zhang Tel 86 10 65296423 E mail junwu zhang pumc edu cn Lineage differentiation is a continuous process during which fated progenitor cells execute specific programs to produce mature counterparts This lineage restricted pathway can be controlled by particular regulators which are usually exclusively expressed in certain cell types or at specific differentiation stages Here we report that miR 376a participates in the regulation of the early stages of human erythropoiesis by targeting cyclin dependent kinase 2 CDK2 and Argonaute 2 Ago2 Among various human leukemia cell lines miR 376a was only detected in K562 cells which originated from a progenitor common to the erythroid and megakaryotic lineages Enforced expression of miR 376a or silencing of CDK2 and Ago2 by RNAi inhibits erythroid differentiation of K562 cells Hematopoietic progenitor cells transduced with miR 376a showed a significant reduction of their erythroid clonogenic capacity MiR 376a is relatively abundant in erythroid progenitor cells where it reduces expression of CDK2 and maintains a low level of differentiation due to cell cycle arrest and decreased cell growth Following erythroid induction miR 376a is significantly down regulated and CDK2 is

    Original URL path: http://www.cell-research.com/arts.asp?id=315 (2016-02-14)
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  • Cell Research
    for Biological Sciences Chinese Academy of Sciences Shanghai 200031 China 4 Graduate School of the Chinese Academy of Sciences Beijing 100039 China 5 Shanghai MOST Key Laboratory of Health and Disease Genomics Chinese National Human Genome Center at Shanghai Shanghai 201203 China 6 National Institute for Communicable Disease Control and Prevention Chinese Center for Disease Control and Prevention Changping Beijing 102206 China Correspondence Shengyue Wang Yixue Li Rong Zeng Rong Zeng Xiao Kui Guo E mail wangsy chgc sh cn yxli sibs ac cn zr sibs ac cn lixuan sippe ac cn microbiology sjtu edu cn The virulence attenuated Leptospira interrogans serovar Lai strain IPAV was derived by prolonged laboratory passage from a highly virulent ancestral strain isolated in China We studied the genetic variations of IPAV that render it avirulent via comparative analysis against the pathogenic L interrogans serovar Lai strain 56601 The complete genome sequence of the IPAV strain was determined and used to compare with and then rectify and reannotate the genome sequence of strain 56601 Aside from their highly similar genomic structure and gene order a total of 33 insertions 53 deletions and 301 single nucleotide variations SNVs were detected throughout the genome of IPAV directly affecting 101 genes either in their 5 upstream region or within their coding region Among them the majority of the 44 functional genes are involved in signal transduction stress response transmembrane transport and nitrogen metabolism Comparative proteomic analysis based on quantitative liquid chromatography LC MS MS data revealed that among 1 627 selected pairs of orthologs 174 genes in the IPAV strain were upregulated with enrichment mainly in classes of energy production and lipid metabolism In contrast 228 genes in strain 56601 were upregulated with the majority enriched in the categories of protein translation and DNA replication repair The combination

    Original URL path: http://www.cell-research.com/arts.asp?id=316 (2016-02-14)
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