archive-com.com » COM » L » LABLIT.COM

Total: 202

Choose link from "Titles, links and description words view":

Or switch to "Titles and links view".
  • I don’t get out much
    March 2015 Lab Twitterati Links LabLit home Categories Comics Drugs Essays Fiction Films Guest post Lab LabLit Meta Podcasts Reviews Students Uncategorized Video Wide world Archives Archives Select Month March 2016 1 January 2016 2 December 2015 1 October 2015 1 June 2015 1 February 2015 2 January 2015 1 December 2014 1 October 2014 1 September 2014 1 June 2014 1 April 2014 1 March 2014 2 February 2014 1 January 2014 2 December 2013 1 November 2013 1 October 2013 1 September 2013 2 August 2013 1 July 2013 1 April 2013 1 January 2013 1 December 2012 3 July 2012 1 May 2012 1 March 2012 1 January 2012 1 November 2011 1 October 2011 1 September 2011 1 August 2011 1 July 2011 1 June 2011 2 May 2011 4 April 2011 2 March 2011 2 February 2011 3 January 2011 1 December 2010 2 November 2010 3 October 2010 2 September 2010 3 August 2010 14 July 2010 6 June 2010 4 May 2010 6 April 2010 5 March 2010 3 February 2010 3 January 2010 6 December 2009 5 November 2009 5 October 2009 10 September 2009 14 August 2009 8 Admin Log in Entries RSS Comments RSS WordPress org I don t get out much September 16th 2011 by Jenny in Uncategorized You know you re a hopeless geek when you get served an onion ring like this and all you can think about is bacterial plasmid replication forks 4 Comments Jim 1 Comment permalink September 16th 2011 at 14 12 Sighs I m with you on that one Jenny I would even go so far as to keep that onion ring for use in tutorials in the hope that it may engage the students more than me drawing a theta on a

    Original URL path: http://blogs.lablit.com/2011/09/i-dont-get-out-much/ (2016-04-26)
    Open archived version from archive

  • Downing tools
    be ten years old on 7 March 2015 Lab Twitterati Links LabLit home Categories Comics Drugs Essays Fiction Films Guest post Lab LabLit Meta Podcasts Reviews Students Uncategorized Video Wide world Archives Archives Select Month March 2016 1 January 2016 2 December 2015 1 October 2015 1 June 2015 1 February 2015 2 January 2015 1 December 2014 1 October 2014 1 September 2014 1 June 2014 1 April 2014 1 March 2014 2 February 2014 1 January 2014 2 December 2013 1 November 2013 1 October 2013 1 September 2013 2 August 2013 1 July 2013 1 April 2013 1 January 2013 1 December 2012 3 July 2012 1 May 2012 1 March 2012 1 January 2012 1 November 2011 1 October 2011 1 September 2011 1 August 2011 1 July 2011 1 June 2011 2 May 2011 4 April 2011 2 March 2011 2 February 2011 3 January 2011 1 December 2010 2 November 2010 3 October 2010 2 September 2010 3 August 2010 14 July 2010 6 June 2010 4 May 2010 6 April 2010 5 March 2010 3 February 2010 3 January 2010 6 December 2009 5 November 2009 5 October 2009 10 September 2009 14 August 2009 8 Admin Log in Entries RSS Comments RSS WordPress org Downing tools August 20th 2011 by Jenny in Uncategorized It s holiday time Yesterday I finished up my work in the lab put my cells to bed cleaned off my desk and headed down to South Kensington for jazz and cocktails with the Labliterati On Monday I m off for an entire week It s always a strange feeling taking time away from the lab You get into these rhythms piling on the experiments until you hardly have time to eat sit down or take comfort breaks Their

    Original URL path: http://blogs.lablit.com/2011/08/downing-tools/ (2016-04-26)
    Open archived version from archive

  • Spaced out
    1 July 2013 1 April 2013 1 January 2013 1 December 2012 3 July 2012 1 May 2012 1 March 2012 1 January 2012 1 November 2011 1 October 2011 1 September 2011 1 August 2011 1 July 2011 1 June 2011 2 May 2011 4 April 2011 2 March 2011 2 February 2011 3 January 2011 1 December 2010 2 November 2010 3 October 2010 2 September 2010 3 August 2010 14 July 2010 6 June 2010 4 May 2010 6 April 2010 5 March 2010 3 February 2010 3 January 2010 6 December 2009 5 November 2009 5 October 2009 10 September 2009 14 August 2009 8 Admin Log in Entries RSS Comments RSS WordPress org Spaced out July 26th 2011 by Jenny in Uncategorized You know it s time to wrap up your post doctoral stint when your hard drive is full I ve been in this lab a bit more than four years having bought a MacBook Pro soon after my arrival This morning I tried to perform a software update and was informed that the start up disk did not contain enough room I think this is a fitting metaphor for something 6 Comments Nik Papageorgiou 1 Comment permalink July 26th 2011 at 16 45 Time to delete and make space for something new Jenny 2 Comment permalink July 26th 2011 at 16 53 In my brain Amen El Ricardipod 3 Comment permalink August 5th 2011 at 00 57 I remember trying to write my thesis on a shared PowerMac oh goodness the glory days I attempted to use Photoshop to crop a scan of a blot and was presented with an out of disk space for swapfile message or something similar The enormous hard disk which was probably all of 1 Gigabyte or so

    Original URL path: http://blogs.lablit.com/2011/07/spaced-out/ (2016-04-26)
    Open archived version from archive

  • Feeling a bit flat
    to perform it straightaway without faffing around with the cloning step Earlier this week I requested a plasmid from a very big very famous American lab I was charmed that the professor answered personally on the same day he received the email and that he immediately instructed his post doc to send it out I was further charmed by the friendliness of the post doc who emailed to say he d sent it Fedex that very same day It had not escaped my notice though that if the plasmid arrived today and there was a goodly amount I could perform my experiment this afternoon and have slides ready on Monday which in turn could be imaged and analyzed before I went on holiday that coming weekend giving me a significant edge on my paper revision deadline I wouldn t dare have asked for more than a dried spot but I was sort of hoping the post doc would sense my desperate fellow post doc angst vibes across the many miles between us And so it was that the Fedex package arrived And I knew immediately how the DNA had been sent the package was flat as a pancake I fondled it optimistically but there was definitely no tube in there not even one of those weeny PCR sized tubes Ah well I thought philosophically as I roused the bugs from their dormant sleep in the 70 freezer It s definitely possible to be too greedy The lab in question probably sends out hundreds of plasmids a week and it s just not practical to feed the five thousand In fact the post doc was doing me a favor I have way too much on on Monday and another experiment probably would have tipped the balance Besides I like bacterial work it reminds me of my lost youth as a bona fide microbiologist So let s hear it for the kindness of strangers something that definitely makes the scientific world go round 12 Comments steve caplan 1 Comment permalink June 12th 2011 at 13 56 Somewhat surprisingly many of my colleagues on your side of the pond not only request dried DNA rather than ready to go DNA plasmids but they actually also request that the material be sent by royal mail rathen than courier Apparently they either fear customs delays or want to save money Any insight from your angle Jenny 2 Comment permalink June 12th 2011 at 14 58 The tube isn t going to change the weight appreciably if it s small and filled with 20 30 uL of liquid is it We don t have customs problems with DNA only Drosophila which routinely arrive dead from the States after their extended holiday at Heathrow Airport Jenny 3 Comment permalink June 12th 2011 at 14 59 Actually I ve just thought the lab head would almost certainly prefer the filter paper it s not like he has to actually prep the damned thing I think all postdocs and

    Original URL path: http://blogs.lablit.com/2011/06/feeling-a-bit-flat/ (2016-04-26)
    Open archived version from archive

  • Tell it like it is
    2013 1 December 2012 3 July 2012 1 May 2012 1 March 2012 1 January 2012 1 November 2011 1 October 2011 1 September 2011 1 August 2011 1 July 2011 1 June 2011 2 May 2011 4 April 2011 2 March 2011 2 February 2011 3 January 2011 1 December 2010 2 November 2010 3 October 2010 2 September 2010 3 August 2010 14 July 2010 6 June 2010 4 May 2010 6 April 2010 5 March 2010 3 February 2010 3 January 2010 6 December 2009 5 November 2009 5 October 2009 10 September 2009 14 August 2009 8 Admin Log in Entries RSS Comments RSS WordPress org Tell it like it is May 26th 2011 by Jenny in Uncategorized So a few of us were sitting around waiting for the weekly departmental seminar to kick off this afternoon and we got to talking about nerves After all giving a public talk can be a stressful experience as your research is put at the mercy of an often hostile audience A post doc in our lab was giving the first talk and I told him he was lucky because our supervisor is out of town Shouldn t that be more stressful he asked Well not to me I explained that when you re talking about your research and the boss isn t in the room you are by default the one who knows more about it than anyone else in the world But if the boss is there I am always aware of everything I say and somewhere in the back of my mind wondering whether it is passing muster whether our world views on the data in question actually coincide My boss has a tell offered one of the other post docs who d arrived early When

    Original URL path: http://blogs.lablit.com/2011/05/tell-it-like-it-is/ (2016-04-26)
    Open archived version from archive

  • Waste not, want not
    14 July 2010 6 June 2010 4 May 2010 6 April 2010 5 March 2010 3 February 2010 3 January 2010 6 December 2009 5 November 2009 5 October 2009 10 September 2009 14 August 2009 8 Admin Log in Entries RSS Comments RSS WordPress org Waste not want not April 19th 2011 by Jenny in Uncategorized My intrepid benchmate had a bit of a rough time today Nothing she lamented seemed to be working She came in to an incubator full of agar plates with nothing growing on them which apparently set the tone for the rest of the day unfolding as it did with various minor disasters and pesky obstacles Nothing devastating just enough to annoy But she was most concerned about her blank plates seven entirely different supercoiled plasmids all zapped into expensive competent bugs from a reputable company starting with S What she speculated might have gone wrong We talked through her protocol the bugs were working fine because I d used them myself only a few days ago Her transformation manipulations were text book and the DNA itself was above reproach We were both stumped But then she slowly reached for her lab notebook and muttered Hang on a minute A few moments of page shuffling and plate examination ensued And then Oh damn Somehow she d plated the two kanamycin resistant bugs on ampicillin plates and the five ampicillin resistant bugs on kanamycin Ouch But fortunately there was a happy ending She realized that the tubes full of bugs were still somewhere in the bin underneath our bench Scrabbling through the trash gloves and pipettes flying every which way she finally managed to find all seven tubes and was able to restreak all of her transformed cultures onto fresh plates I told her if she really wanted to go for it she should re use the plates that nothing had grown on But she wasn t too impressed with that idea The purists may frown but I ll bet they grow Stay tuned 8 Comments rpg 1 Comment permalink April 19th 2011 at 22 37 Ha ha I ve done that And recovered it the same way I don t recall being quite brave enough to re use the plates though Nik Papageorgiou 2 Comment permalink April 20th 2011 at 11 45 I m a purist I m frowning Ha can t even write that with a straight face Bugs should grow fine Not like wimpy mammalian cells oooh no I ve contacted air outside the lamina hood oooh noooo I m contaminated ooooh noooo Bugs know no fear Bugs are survivors Jenny 3 Comment permalink April 20th 2011 at 12 07 Lots of juicy colonies this morning Hurrah rpg 4 Comment permalink April 20th 2011 at 12 13 Huzzah nico 5 Comment permalink April 20th 2011 at 12 59 Been there done that but unfortunately never came across the recovery method in any paper There should be a rule in all labs wait

    Original URL path: http://blogs.lablit.com/2011/04/waste-not-want-not/ (2016-04-26)
    Open archived version from archive

  • Uncategorized - Lab Literal
    could you want Christmas escapism searching for CP Snow LabLit chats with Anna Ziegler Summer reading LabLit com will be ten years old on 7 March 2015 Lab Twitterati Links LabLit home Categories Comics Drugs Essays Fiction Films Guest post Lab LabLit Meta Podcasts Reviews Students Uncategorized Video Wide world Archives Archives Select Month March 2016 1 January 2016 2 December 2015 1 October 2015 1 June 2015 1 February 2015 2 January 2015 1 December 2014 1 October 2014 1 September 2014 1 June 2014 1 April 2014 1 March 2014 2 February 2014 1 January 2014 2 December 2013 1 November 2013 1 October 2013 1 September 2013 2 August 2013 1 July 2013 1 April 2013 1 January 2013 1 December 2012 3 July 2012 1 May 2012 1 March 2012 1 January 2012 1 November 2011 1 October 2011 1 September 2011 1 August 2011 1 July 2011 1 June 2011 2 May 2011 4 April 2011 2 March 2011 2 February 2011 3 January 2011 1 December 2010 2 November 2010 3 October 2010 2 September 2010 3 August 2010 14 July 2010 6 June 2010 4 May 2010 6 April 2010 5 March 2010

    Original URL path: http://blogs.lablit.com/category/uncategorized/page/4/ (2016-04-26)
    Open archived version from archive

  • Lab Literal
    is out of town Shouldn t that be more stressful he asked Well not to me I explained that when you re talking about your research and the boss isn t in the room you are by default the one who knows more about it than anyone else in the world But if the boss is there I am always aware of everything I say and somewhere in the back of my mind wondering whether it is passing muster whether our world views on the data in question actually coincide My boss has a tell offered one of the other post docs who d arrived early When you re up at the podium and you say something not quite right he does this And she proceeded to demonstrate hand creeping over eyes hunched shoulders a fatal shrinking in of her entire body And if you don t extract yourself correctly she added he starts fiddling with his socks My colleague then recalled that our supervisor also has a tell he leans back in his chair tilts his head and raises his eyebrows to a rather non committal elevation On second thought he said Maybe it is a good thing he s out of town 2 Comments add yours How to write a paper in 12 easy steps fin May 20th 2011 by rpg in Comics By Nik Papageorgiou Comments Off on How to write a paper in 12 easy steps fin How to write a paper in 12 easy steps Part 2 May 18th 2011 by rpg in Comics By Nik Papageorgiou 2 Comments add yours How to write a paper in 12 easy steps Part 1 May 16th 2011 by rpg in Comics By Nik Papageorgiou 2 Comments add yours What s it all about April 25th 2011 by rpg in Comics By Nik Papageorgiou 2 Comments add yours Waste not want not April 19th 2011 by Jenny in Uncategorized My intrepid benchmate had a bit of a rough time today Nothing she lamented seemed to be working She came in to an incubator full of agar plates with nothing growing on them which apparently set the tone for the rest of the day unfolding as it did with various minor disasters and pesky obstacles Nothing devastating just enough to annoy But she was most concerned about her blank plates seven entirely different supercoiled plasmids all zapped into expensive competent bugs from a reputable company starting with S What she speculated might have gone wrong We talked through her protocol the bugs were working fine because I d used them myself only a few days ago Her transformation manipulations were text book and the DNA itself was above reproach We were both stumped But then she slowly reached for her lab notebook and muttered Hang on a minute A few moments of page shuffling and plate examination ensued And then Oh damn Somehow she d plated the two kanamycin resistant bugs on ampicillin plates and the five ampicillin resistant bugs on kanamycin Ouch But fortunately there was a happy ending She realized that the tubes full of bugs were still somewhere in the bin underneath our bench Scrabbling through the trash gloves and pipettes flying every which way she finally managed to find all seven tubes and was able to restreak all of her transformed cultures onto fresh plates I told her if she really wanted to go for it she should re use the plates that nothing had grown on But she wasn t too impressed with that idea The purists may frown but I ll bet they grow Stay tuned 8 Comments add yours No news is good news March 31st 2011 by Jenny in Uncategorized So I ve just submitted my paper the culmination of 4 years of hard scientific work to a very good journal It s Thursday afternoon nearly Friday I feel like I ve been run over by a tank And I find myself thinking I don t care if it gets sent out for review and ultimately gets rejected I m just happy that don t have to think about it for a while So go ahead referees take as long as you like Really 6 Comments add yours Industrial dis ease March 12th 2011 by rpg in Guest post Wide world Guest post from Nik Papageorgiou Every culture has taboos There s a dark side to the Force And for every mythology there s always some kind of power dominion or netherworld whose name must never be uttered or only with solemn care And I d like to think that by now scientists have realized that they are not exempt from such cultural dynamics after all we have all the identifying marks of separate culture traditions rites of passage ceremonies unspoken rules spoken rules succession sensitivity to stereotyping hierarchical structures and of course taboos So today we fiddle with the hex It s true that given scientific economics are in the bust phase of their cycle industry does and will continue to attract scientists from academia There are other many and varied incentives but in many cases industry is simply the only way to go I currently work in industry It s not the first time I also did an MSc industrial placement which I remember as the best funded research I ve ever carried out Only 2 000 Buy two And I ve yet to meet the scientist whose childhood dream was to work in a pharmaceutical or biotech company But I ve met many who dreamt they were falling and had to wake up before they hit the ground For a long time industry has a stigma in academic circles It is regarded as either a last refuge for those who couldn t hack it or an end of innocence a less than happy finale to the academic fairy tale Back in my PhD days I remember discussing a student who d accepted an industrial position It was a

    Original URL path: http://blogs.lablit.com/page/5/ (2016-04-26)
    Open archived version from archive



  •