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  • Day 4 (Alex’s Version)
    14 July 2010 6 June 2010 4 May 2010 6 April 2010 5 March 2010 3 February 2010 3 January 2010 6 December 2009 5 November 2009 5 October 2009 10 September 2009 14 August 2009 8 Admin Log in Entries RSS Comments RSS WordPress org Day 4 Alex s Version August 12th 2010 by Alex in Students It s day four and tensions are rising was what Jenny said today refering to Alan s and my rising confusion and stress levels as we tried to finish the western blot and stain our gene knockdown cells all at the same time Trying to remember what measurements applied to which liquids and what bits of the experiment we had and hadn t already done was certainly starting to fry my brain and possibly Alan s too Today we encountered a new room the cold room where we d left the western blot membranes overnight this is literally a giant walk in fridge It was pretty cool in both senses until we realised to our horror that there was no panic switch I can imagine how you d feel if you accidentally got locked inside it was so cold We then got down to the serious business of preserving our HeLa cells in formaldehyde and then washing it off countless times back in the lab It was quite theraputic once you got into the rhythm of suctioning off all the liquid in the wells and then pipetting in some more After we finally got that done it was back to the western blot and of course passing through the tardis down into the dark room This time Alan and I got to have a go at making the images I did a ten second one and then Alan did a thirty second

    Original URL path: http://blogs.lablit.com/2010/08/day-4-alexs-version/ (2016-04-26)
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  • Day 1: Extreme maths (video clip)
    2013 1 December 2012 3 July 2012 1 May 2012 1 March 2012 1 January 2012 1 November 2011 1 October 2011 1 September 2011 1 August 2011 1 July 2011 1 June 2011 2 May 2011 4 April 2011 2 March 2011 2 February 2011 3 January 2011 1 December 2010 2 November 2010 3 October 2010 2 September 2010 3 August 2010 14 July 2010 6 June 2010 4 May 2010 6 April 2010 5 March 2010 3 February 2010 3 January 2010 6 December 2009 5 November 2009 5 October 2009 10 September 2009 14 August 2009 8 Admin Log in Entries RSS Comments RSS WordPress org Day 1 Extreme maths video clip August 12th 2010 by rpg in Students Video Just like in the films There s one more vid of day 1 to come then I need to process and upload the rest of the video There s a lot 5 Comments Nige 1 Comment permalink August 12th 2010 at 08 46 This is the thing Dilutions Be it cells or drugs this hurdle is one of the biggest simple early problems that any lab scientist faces I remember learning it in Pharmacology 3 1 4 at Aberdeen and later I remember teaching it in Pharmacology classes at Liverpool In the end I used to use a method that involved working out the single massive dilution factor needed and then breaking it down into serials Certainly that was the easiest way to teach it Jenny 2 Comment permalink August 12th 2010 at 08 54 I was pretty amazed when Alan worked out that if you put your desired concentration and your current concentration into the same scientific notation e g 1 x 10 6 and 345 x 10 6 then you could disregard the 10

    Original URL path: http://blogs.lablit.com/2010/08/day-1-extreme-maths-video-clip/ (2016-04-26)
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  • Days 2 and 3 (Alex’s Version)
    general If it was the latter it would be fine But we could not be sure this was the case until we tested it with a completely different antibody that should show up the same on all the samples if the control truly was protein deficient then the x ray line would be fainter We shall find out tomorrow the mystery plot continues And now we come to the highlight of the day the super confocal microscope It was absolutely awesome We went downstairs with Jenny and her collection of slides to a small room with a cool swanky looking microscope which had a separate set of dials and was linked up to a computer where we could see the images of the cells The first one was a normal control cell where they all looked round and happy Jenny then put on a slide where a gene had been knocked out and this had made the cells all spiky And they were so spiky So we got to search for a cell that looked particularly impressive and when we put it up on the screen I swear it looked like a person Well a cell with a bit sticking out that looked a bit like a head with a very spiky torso two misshapen arms one leg and a tail We named it Spike which we thought was very appropriate But the next one we found was even more impressive being spikier and more dramatic than Spike Perhaps no one else really saw it this way but I was pretty sure that it looked like a very spiky bird on a very thorny branch with a huge strange pointy slug sitting randomly in the top right corner of the field But it got a name too we called it Tweet I used my phone to take loads of photos of Jenny and Alan doing cool things in the lab mostly working in the hood and making the gel for the proteins but unfortunately they seem to be stuck in there and so I can t show them to everyone yet Though hopefully I ll be able to post the images from the microscope soon They looked so amazing and beautiful and as they looked quite alien for once I think calling them out of this world really is appropriate So I m looking forward to another day in the lab and with any luck we ll get to see our results And then the day after that we ll see our cells that have had a gene knocked down and I ll be able to see how getting rid of Jenny s pet gene affects the shape of my cells 9 Comments rpg 1 Comment permalink August 12th 2010 at 08 35 It s all good fun until somebody clones an army of radioactive gorillas and holds the world to ransom Nige 2 Comment permalink August 12th 2010 at 08 55 Jenny Alex Alan This whole idea of blogging

    Original URL path: http://blogs.lablit.com/2010/08/days-2-and-3-alexs-version/ (2016-04-26)
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  • Day 1: This is how it’s done (video clip)
    reading LabLit com will be ten years old on 7 March 2015 Lab Twitterati Links LabLit home Categories Comics Drugs Essays Fiction Films Guest post Lab LabLit Meta Podcasts Reviews Students Uncategorized Video Wide world Archives Archives Select Month March 2016 1 January 2016 2 December 2015 1 October 2015 1 June 2015 1 February 2015 2 January 2015 1 December 2014 1 October 2014 1 September 2014 1 June 2014 1 April 2014 1 March 2014 2 February 2014 1 January 2014 2 December 2013 1 November 2013 1 October 2013 1 September 2013 2 August 2013 1 July 2013 1 April 2013 1 January 2013 1 December 2012 3 July 2012 1 May 2012 1 March 2012 1 January 2012 1 November 2011 1 October 2011 1 September 2011 1 August 2011 1 July 2011 1 June 2011 2 May 2011 4 April 2011 2 March 2011 2 February 2011 3 January 2011 1 December 2010 2 November 2010 3 October 2010 2 September 2010 3 August 2010 14 July 2010 6 June 2010 4 May 2010 6 April 2010 5 March 2010 3 February 2010 3 January 2010 6 December 2009 5 November 2009 5 October 2009

    Original URL path: http://blogs.lablit.com/2010/08/day-1-this-is-how-its-done-video-clip/ (2016-04-26)
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  • The first steps
    September 2014 1 June 2014 1 April 2014 1 March 2014 2 February 2014 1 January 2014 2 December 2013 1 November 2013 1 October 2013 1 September 2013 2 August 2013 1 July 2013 1 April 2013 1 January 2013 1 December 2012 3 July 2012 1 May 2012 1 March 2012 1 January 2012 1 November 2011 1 October 2011 1 September 2011 1 August 2011 1 July 2011 1 June 2011 2 May 2011 4 April 2011 2 March 2011 2 February 2011 3 January 2011 1 December 2010 2 November 2010 3 October 2010 2 September 2010 3 August 2010 14 July 2010 6 June 2010 4 May 2010 6 April 2010 5 March 2010 3 February 2010 3 January 2010 6 December 2009 5 November 2009 5 October 2009 10 September 2009 14 August 2009 8 Admin Log in Entries RSS Comments RSS WordPress org The first steps August 10th 2010 by Alan in Students So I ve spent two days working at MRC and it s been very action packed The first morning was spent learning the basics as shown in the wonderful video footage But we soon got to knocking down our genes and working under the hood which was cool as it s one of those things you always see the TV scientists doing But it wasn t until this morning that we got to have a good look at our cells and see how they were doing As Alex has previously said we did have a slight problem with the concentrations resulting in a bit of overcrowding but it wasn t too bad and this morning the cells were looking good The afternoon was spent helping Jenny with her Western Blot procedure I actually got to make one of the sandwich

    Original URL path: http://blogs.lablit.com/2010/08/the-first-steps/ (2016-04-26)
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  • Day 1: Ready to do science (Video clip)
    Ziegler Summer reading LabLit com will be ten years old on 7 March 2015 Lab Twitterati Links LabLit home Categories Comics Drugs Essays Fiction Films Guest post Lab LabLit Meta Podcasts Reviews Students Uncategorized Video Wide world Archives Archives Select Month March 2016 1 January 2016 2 December 2015 1 October 2015 1 June 2015 1 February 2015 2 January 2015 1 December 2014 1 October 2014 1 September 2014 1 June 2014 1 April 2014 1 March 2014 2 February 2014 1 January 2014 2 December 2013 1 November 2013 1 October 2013 1 September 2013 2 August 2013 1 July 2013 1 April 2013 1 January 2013 1 December 2012 3 July 2012 1 May 2012 1 March 2012 1 January 2012 1 November 2011 1 October 2011 1 September 2011 1 August 2011 1 July 2011 1 June 2011 2 May 2011 4 April 2011 2 March 2011 2 February 2011 3 January 2011 1 December 2010 2 November 2010 3 October 2010 2 September 2010 3 August 2010 14 July 2010 6 June 2010 4 May 2010 6 April 2010 5 March 2010 3 February 2010 3 January 2010 6 December 2009 5 November 2009 5

    Original URL path: http://blogs.lablit.com/2010/08/day-1-ready-to-do-science-video-clip/ (2016-04-26)
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  • Day One (Alex’s version)
    it was a gun We then tried and I personally failed to open flasks with one hand I watched with envy as Alan calmly unscrewed the lid and expertly put it back on whereas I could just about manage to take the lid off but failed to put it back on again Jenny had to keep holding my right hand down but I got it eventually But after just a little messing around we got straight to work with Jenny helping a lot of course Today we knocked out a gene in a cell and then on Friday we get to see how the absence of the protein that this gene coded for has affected the cell In brief we took some cancer cells and by some I mean probably several thousand and we mixed them with a solution to separate them from each other and from the dish they were on We then had to count them using a slide with a grid which in itself was no problem at all but alas after having done no maths for several weeks we had fun trying to work out how much liquid we had to add take away to get to the concentration we were supposed to have Needless to say we eventually got the right concentration but not before we discovered that we actually got it wrong the first time when Alan added his cells and there were way too many Finally after centrifuging incubating and lots of pipetting RNA and some medium from plastic tubes onto our slide we were able to put our slides away and start the 3 day wait I had so much fun today I got to do a real proper lab experiment Things that are probably the most every day in the world to a lot of scientists but it was completely new and an amazing experience for me knowing that this wasn t just a practical in school but some real lab work And it was particularly funny when Jenny kept randomly appearing with her video camera filming some of our stressful yet successful endeavours There was one special moment where Jenny accidentally dropped a bottle of saline solution though to be fair she had severe jet lag It was only when I got home when I realized how absolutely exhausted I was But I can t wait to go back tomorrow 15 Comments Jenny 1 Comment permalink August 9th 2010 at 20 21 You weren t supposed to tell anyone I dropped that bottle Alex 2 Comment permalink August 9th 2010 at 20 40 Hahaha i knooow but you said you were going to cut that bit out of the footage I shot Therefore I had to say it rpg 3 Comment permalink August 9th 2010 at 20 51 You should know that I m editing the footage Every man has his price just sayin Jenny 4 Comment permalink August 9th 2010 at 20 54 I ve got a sinking feeling

    Original URL path: http://blogs.lablit.com/2010/08/day-one-alexs-version/ (2016-04-26)
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  • Work experience time!
    2012 1 November 2011 1 October 2011 1 September 2011 1 August 2011 1 July 2011 1 June 2011 2 May 2011 4 April 2011 2 March 2011 2 February 2011 3 January 2011 1 December 2010 2 November 2010 3 October 2010 2 September 2010 3 August 2010 14 July 2010 6 June 2010 4 May 2010 6 April 2010 5 March 2010 3 February 2010 3 January 2010 6 December 2009 5 November 2009 5 October 2009 10 September 2009 14 August 2009 8 Admin Log in Entries RSS Comments RSS WordPress org Work experience time August 9th 2010 by Alex in Students I m a sixth form student studying three sciences and maths for my A levels tough but fun I promise On the 9th of August I am going to start five days of work experience at Dr Jennifer Rohn s lab in UCL I m really excited since I ve never worked in a proper laboratory before I m interested to find out what sort of experiments take place and see how the kind of lab experiments you see on TV compare with the reality I m really looking forward to meeting all the scientists who work there I m hoping they ll be fun and interesting to talk to and that perhaps one or two will let me interview them for my extended project so that I could collect some different opinions on my project topic which is future treatments for cancer I m really glad that I have this opportunity to work in a lab not only because it is good experience but also because I d be working with a woman scientist which is what I hope to be And it s inspiring to see what I can aspire to and what

    Original URL path: http://blogs.lablit.com/2010/08/work-experience-time/ (2016-04-26)
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