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  • Therion International Web Site

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    Original URL path: /index-zooa.htm (2016-02-12)



  • Therion International Web Site

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    Original URL path: /index-bibl.htm (2016-02-12)


  • Therion International Web Site

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    Original URL path: /index-form.htm (2016-02-12)


  • Therion International Web Site

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    Original URL path: /index-spec.htm (2016-02-12)


  • Therion International

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    Original URL path: /ther-in-aqua.htm (2016-02-12)


  • Therion International Aquaculture Fact Sheet
    transferred to a nylon membrane The membrane was hybridized with Therion s proprietary DNA probe OPT 06 washed and exposed to x ray film The lanes labelled MWSS contain molecular weight sizing standards Three genetic markers yellow bands were observed in 50 75 of C individuals tested but were not observed in any BQ or F salmon One genetic marker green bands was found to occur in all BQ coho but not in any of the other coho populations In addition 75 of F coho salmon exhibited a specific genetic marker blue bands which did not appear in any individuals from the C or BQ population In conclusion Therion s DNA testing services make it possible to readily differentiate among these stock populations Stock Identification Striped Bass A study was conducted to identify genetic markers that could differentiate among individuals representing two populations of striped bass The figure to the left shows the DNA profiles of 11 striped bass generated with probe enzyme combination OPT 05 Hae III All individuals from Population 1 display bands in the region above 6 6 kb None of the individuals from Population 2 have bands in this area thus the two populations can be genetically distinguished by the presence or absence of bands in the region above 6 6 kb Genetic Variation Shrimp A client was concerned about the potential loss of genetic variation within a captive strain of Penaeus stylirostris The figure to the right compares the DNA profiles of six shrimp from a captive population to six shrimp from a wild population These profiles were generated with the probe enzyme combination OPT 05 BstN I Within the captive strain genetic variation is less than 15 note that each genetic marker is shared by nearly all individuals The wild strain shows significantly more

    Original URL path: http://www.theriondna.com/fact-aqua.htm (2016-02-12)
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  • Therion International

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    Original URL path: /ther-in-aqss.htm (2016-02-12)


  • Therion International Aquaculture Species Substitution Fact Sheet
    of DNA sequencing or DNA amplification we have been able to address questions concerning substitutions for red snapper grouper tilapia and walleye In addition the scientists of Therion can develop novel assays to work with any other species of interest Red Snapper or Grouper or In a survey of red snapper served in 39 restaurants across six cities in the Southeastern US it was determined that 19 out of the 39 samples tested were indeed not red snapper but a cheaper species substitution The substituted species included lane snapper scarlet snapper and even tilapia and horse mackerel Similarly in a survey of numerous lots of grouper fillets about to be purchased by an importer distributor it was determined that only a small proportion of fillets were actually from grouper species Among the species commonly substituted were emperor fish lesser snapper species and even sculpins Pictured below are mitochondrial DNA sequences from authentic red snapper and grouper individuals respectively Stock Identification Walleye or Zander Walleye is a popular tasty freshwater fish endemic to lakes in the Northern US and Canada In a study of fish served in 30 restaurants across four cities in the Great Lakes region of the US it was determined that 12 of the 30 cooked fillets tested were actually from zander The zander is European species from the same genus as the walleye which is farmed exported to the US and can be purchased for as much as 2 less per pound than the locally caught walleye Depicted below are results from a DNA amplification assay showing the difference in allelic sizes between walleye and zander In total the assay developed by Therion for these species surveys six independant species specific microsatellite loci Specimen Requirements please call before shipping any samples Specimen Type Volume Container Shipping Instructions

    Original URL path: http://www.theriondna.com/fact-aqss.htm (2016-02-12)
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