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  • Therion International

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    Original URL path: /ther-in-exot.htm (2016-02-12)


  • Therion International Exotic Animals Fact Sheet
    captive nesting pairs of scarlet and Waldrapp ibises The autoradiograph at right depicts the DNA profiles of a nesting pair of scarlet ibises and their chick Note that each genetic marker observed in the DNA profile of the chick can also be observed in the DNA profile of one or both adults thus verifying parentage No scarlet ibis chicks were found to be the product of an extra pair copulation However some Waldrapp chicks were excluded from being the offspring of the mated pair in whose nest they were found Estimation of Genetic Variation To ensure the long term reproductive success and survival of captive bred populations of animals it is essential to routinely monitor levels of genetic variation Pictured below are two sets of DNA profiles probe OPT 05 generated from individuals belonging to two separate colonies of New World primates Population A exhibits high levels of genetic variation as indicated by a low level of band sharing among individuals In contrast population B exhibits a low level of genetic variation as indicated by high levels of band sharing among individuals Note the nearly identical DNA profile banding patterns among individuals of population B DNA profile results such as these can be used to generate estimates of heterozygosity genetic distance and relatedness Estimation of Genetic Relatedness A private breeder of reptiles needed to determine the paternities of a clutch of python snakes The mother of this clutch had been bred to two males and because multiple paternities are common in reptiles it was necessary to identify and partition the offspring into full sibling groups The results were generated using probes OPT 03 and OPT 05 and enzyme HinF I Visual inspection of the DNA profiles showed two subsets of similar banding patterns among the young snakes figure at left

    Original URL path: http://www.theriondna.com/fact-exot.htm (2016-02-12)
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  • Therion International

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    Original URL path: /ther-in-wild.htm (2016-02-12)


  • Therion International Fish & Wildlife Conservation, Forensics and Management Fact Sheet
    parts stolen animals stock and the fraudulent misidentification of fish meat species Two cases involving the illegal hunting of deer and black bear are depicted to the left and right respectively In both cases DNA samples collected from meat or tissue found in the suspects possession were tested to determine genetic similarity with forensics samples collected at the site of the kill Note that in each case the DNA profile banding patterns matched across every sample collected by case investigators Variation in the intensity or clarity of the banding patterns is due to the quality of the DNA obtained from the forensics samples Results from a given set of forensics samples can be compared to database sample sets to allow for statistical analysis In the vast majority of cases for which Therion has provided DNA evidence the courts have stipulated to the final written report In the few remaining cases the senior scientific staff of Therion was able to provide successful expert testimony Genetic Variation Within Among Populations Bald Eagle In cooperation with several bald eagle ecology and recovery programs a bald eagle genetic database was compiled to estimate levels of genetic variation within and among populations Well over 300 samples were submitted by field scientists studying resident populations throughout North America DNA profiles were prepared using restriction enzyme Hae III and Therion s multi locus probes OPT 03 OPT 04 and OPT 05 The figure at right depicts the DNA profiles of two siblings P1S1 P1S2 another bald eagle from the same population P1 and a single individual from a different population P2 The lane labelled MWSS contains molecular weight sizing standards Note the varying degrees of similarities among the banding patterns Therion used proprietary computer softwares to calculate band sharing coefficients and estimate heterozygosity and genetic distance from

    Original URL path: http://www.theriondna.com/fact-wild.htm (2016-02-12)
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  • Therion International

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    Original URL path: /ther-in-laba.htm (2016-02-12)


  • Therion International Laboratory Animal Fact Sheet
    and personnel costs Estimation of Transgene Copy Number Predicting Expression Level This pioneering technology allows investigators to predict expression levels of transgenes in young founder animals and prior to actual expression and or phenotyping With this knowledge projected non expressing founders can be culled prior to breeding thereby substantially reducing costs and work load Figure 2 shows the analysis of four founders from a given transgenic line Quantified amounts of DNA from each founder were processed in a Southern blot assay which was hybridized with DNA probes for both the transgene and an endogenous single copy gene Optical density measurements were taken on all resulting bands and estimates of copy numbers were computed by comparison of transgene bands blue dots to both in lane blue arrows and external red arrows endogenous controls Note the varying intensities of the transgene bands To date Therion has tested well over 100 transgenic models In 80 of these cases the mice that ultimately reached a required threshold level of expression were those individuals whose copy numbers estimates were among the three highest values In the remaining cases ultimately none of the founders expressed the transgene Transgenic Monitoring for Carrier Status Therion offers a comprehensive set of DNA based tests to facilitate the periodic monitoring of both transgenic and knockout strains of laboratory animals These tests include DNA amplification Southern blot and slot blot assays Our ability to transfer and implement existing protocols will relieve your laboratory of the tedious task of genetic monitoring Our scientists also have the expertise to enhance existing assays and or develop new assays to meet your specifications Our prices and turn around times for these services are extremely competitive Estimation of Genetic Variation To ensure the long term reproductive success of captive bred populations of animals used for biomedical

    Original URL path: http://www.theriondna.com/fact-laba.htm (2016-02-12)
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  • Therion International

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    Original URL path: /ther-in-dome.htm (2016-02-12)


  • Therion International Pets and Domestic Animal Fact Sheet
    Stallion B is therefore clearly shown to be the true sire of the foals Canine Sire Verification A greyhound bitch was believed to have been inseminated by two males To verify sire identity DNA profiles were produced from the dam her pups and the male to which she had been purposely bred The DNA probe used was OPT 05 and a portion of the autoradiograph is shown to the left Note that a genetic marker yellow band found in the DNA profile of pup 1 appeared neither in the dam s profile nor in that of the alleged sire This confirmed that the female had been inseminated by a second male and excluded the preferred male as sire of the pup Other genetic markers and additional DNA probe assays corroborated this conclusion Estimation of Genetic Relatedness Macaws A breeder of macaws had recently purchased three young and allegedly unrelated birds one male and two females to add to her breeding flock Since the male and female B were constantly observed together the breeder wondered whether she should allow these birds to become a mated pair DNA profile testing was conducted to determine the relatedness of the three macaws The DNA profile results in the figure to the right show a high proportion of shared bands or genetic markers blue arrows between the male and female B It was concluded that the male and female B were probably related possibly siblings and therefore should not be paired Pythons A private breeder of reptiles needed to determine the paternities of a clutch of python snakes The mother of this clutch had been bred to two males and because multiple paternities are common in reptiles it was necessary to identify and partition the offspring into full sibling groups The results were generated using

    Original URL path: http://www.theriondna.com/fact-dome.htm (2016-02-12)
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